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Prominence preparative hplc system

Manufactured by Shimadzu
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Sourced in Japan, United States

The Prominence preparative HPLC system is a high-performance liquid chromatography instrument designed for preparative-scale purification of chemical compounds and biomolecules. It is capable of efficiently separating and collecting target analytes from complex mixtures.

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Lab products found in correlation

Spd 20a uv detector, by Shimadzu (2 mentions) Waters 2695 separations module, by Waters Corporation (1 mentions) 177lucl3, by PerkinElmer (1 mentions) Actus triart c18 column, by YMC (1 mentions) Dota nhs ester, by Macrocyclics (1 mentions) C18 guard column, by Phenomenex (1 mentions) Luna c18, by Phenomenex (1 mentions)

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Preparative HPLC system Prominence HPLC system Preparative HPLC Prominence HPLC Prominence system HPLC system

4 protocols using prominence preparative hplc system

1

Preparative HPLC Fractionation and Analysis

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The extract was fractionated on Prominence preparative HPLC system (Shimadzu Corporation, Kyoto, Japan) and chromatographic separation was performed at 40 °C using a Luna C18 preparative column (5 μm particle size, 150 cm L × 21.20 mm I.D.; Phenomenex, Torrance, CA). HPLC separations for time-based and peak-based fractionation were carried out with H2O (A) and methanol +0.1% Formic acid (B) and, at a flow rate of 10 ml/min. The gradient profile was as follows: 5% to 50% methanol in 5 minutes, followed by 95% methanol for 30 minutes. Fraction 1 (F1) was collected between 7.5 to 15 minutes of elution time, whereas fraction 2 (F2), fraction 3 (F3) and fraction 4 (F4) were collected from 15.2–17 minutes, 17.2–20 minutes and 20.5–23.5 minutes of elution respectively (Fig. 1A). After evaporation of the solvent, all fractions were freeze-dried under reduced pressure and dry powder was dissolved in methanol at a concentration of 1 mg/ml and was used for analysis using HPLC and LC-MS/MS.
Khan N.M., Haseeb A., Ansari M.Y, & Haqqi T.M. (2017). A wogonin-rich-fraction of Scutellaria baicalensis root extract exerts chondroprotective effects by suppressing IL-1β-induced activation of AP-1 in human OA chondrocytes. Scientific Reports, 7, 43789.
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2

Synthesis and Characterization of Radiolabeled Theranostic Agent

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ADS790WS is 2-[2-[2-(4-aminobenzenethio)-3-[(1,3-dihydro-3,3-dimethyl-1-(4-sulfobutyl)-2H-indol-2-ylidene)-ethylidene]-1-cycloxen-1-yl]-ethynyl]-3,3-dimethyl-1-(4-sulfobutyl)-3H-indolium, innersalt, monosodium, which was purchased from American Dye Source, Inc. (Montreal, QC, Canada). DOTA-NHS-ester (1,4,7,10-Tetraazacyclododecane- 1,4,7,10-tetraacetic acid mono-N-hydroxysuccinimide ester) was purchased from Macrocyclics (Dallas, TX, USA). The ESI (electrospray ionization) mass spectral data were collected on a AB Sciex 4000QTrap system (Concord, ON, Canada). 111InCl3 (indium chloride in 0.05 M HCl) was purchased from Institute of Nuclear Energy Research (INER), Taoyuan, Taiwan. 177LuCl3 was purchased from perkin elmer (Waltham, MA, USA).
Preparative reversed-phase high performance liquid chromatography (HPLC) was performed on a SHIMADZU Prominence Preparative HPLC System with a SHIMADZU SPD-20AV detector using YMC-Actus Triart C18 column (5 μm, 250 × 20 mm). Analytic reversed-phase HPLC was performed on a Waters 2695 Separations Module with a Waters 2487 Dual Wavelength Absorbance Detector plus a Bioscan radioisotope detector using YMC-Triart C18 column (5 μm, 250 × 4.6 mm). Waters Bridge column (5 μm, 200 ×4.6 mm). The flow rate was 20 mL/min for the preparative column and 1 mL/min for the analytic column running wth 60% ACN and 40% water with 0.1% TFA.
Peng C.L., Shih Y.H., Chiang P.F., Chen C.T, & Chang M.C. (2021). Multifunctional Cyanine-Based Theranostic Probe for Cancer Imaging and Therapy. International Journal of Molecular Sciences, 22(22), 12214.
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3

Preparative HPLC Purification of Compounds

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All purifications were performed on a Shimadzu Prominence preparative HPLC system, consisting of LC-8A binary solvent pumps, an SCL-10A system controller, a SIL-10AP auto sampler, and an FRC-10A fraction collector. A Shimadzu SPD-20A UV detector set to 214 nm was used for detection. Chromatographic separations were obtained using a Phenomenex Luna C18 preparative column (5 μm, 150 mm × 21.5 mm i.d.) with a Phenomenex C18 column guard (5 μm, 15 mm × 21.2 mm i.d.). Prominence prep software was used to set all detection and collection parameters. The mobile phases for HPLC purification were HPLC grade obtained from Sigma-Aldrich and Fisher Scientific. The mobile phase consisted of a mixture of acetonitrile/water (both with 0.1% trifluoroacetic acid). The initial setting for separation was 2% acetonitrile, which was held for 2 min, then the gradient was linearly increased to 20% acetonitrile over 4 min. The gradient was then linearly increased to 55% acetonitrile over 36 min. The HPLC system was set to automatically flush and re-equilibrate the column after each run for a total of four column volumes. The total flow rate was set to 12 ml/min, and the total injection volume was set to 3900 μl. The fractions corresponding to the desired product were then combined and lyophilized.
Tran T., Chiem K., Jani S., Arivett B.A., Lin D.L., Lad R., Jimenez V., Farone M.B., Debevec G., Santos R., Giulianotti M., Pinilla C, & Tolmasky M.E. (2018). Identification of a Small Molecule Inhibitor of the Aminoglycoside 6’-NAcetyltransferase Type Ib [AAC(6’)-Ib] Using Mixture-Based Combinatorial Libraries. International journal of antimicrobial agents, 51(5), 752-761.
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4

Preparative HPLC Purification of Compounds

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All purifications were performed on a Shimadzu Prominence preparative HPLC system, consisting of LC-8A binary solvent pumps, a SCL-10A system controller, a SIL-10AP auto sampler, and a FRC-10A fraction collector. A Shimadzu SPD-20A UV detector was used for detection. The wavelength was set at 214nm during analysis. Chromatographic separations were obtained using a Phenomenex Luna C18 preparative column (5μm, 150 × 21.5mm i.d.). The column was protected by a Phenomenex C18 column guard (5μm, 15 × 21.2mm i.d.). Prominence prep software was used to set all detection and collection parameters. The mobile phases for HPLC purification were HPLC grade obtained from Sigma Aldrich and Fisher Scientific. The mobile phase consisted of a mixture of Acetonitrile/water (both with 0.1% formic acid). The initial setting for separation was 2% Acetonitrile, which was held for 2 minutes, then the gradient was linearly increased to 20% Acetonitrile over 4 minutes. The gradient was then linearly increased to 55% Acetonitrile over 36minutes. The HPLC system was set to automatically flush and re-equilibrate the column after each run for a total of 4 column volumes. The total flow rate was set to 12mL/min and the total injection volume was set to 3900uL. The fraction collector was set to collect from 6 to 40 minutes. The corresponding fractions were then combined and lyophilized.
Fleeman R., LaVoi T.M., Santos R.G., Morales A., Nefzi A., Welmaker G.S., Medina-Franco J.L., Giulianotti M.A., Houghten R.A, & Shaw L.N. (2015). Combinatorial libraries as a tool for the discovery of novel, broad-spectrum antibacterial agents targeting the ESKAPE pathogens. Journal of medicinal chemistry, 58(8), 3340-3355.
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