Omnifix f

Manufactured by B. Braun

Sourced in Germany

The Omnifix-F is a laboratory equipment product manufactured by B. Braun. It is a syringe designed for accurate and precise fluid handling in a laboratory setting.

Automatically generated - may contain errors

Lab products found in correlation

Sterican, by B. Braun (2 mentions) Hfe 7500 oil, by 3M (2 mentions) Phantom 7, by Ametek (2 mentions) Pump 11 elite, by Harvard Apparatus (1 mentions) Precisionglide needle, by BD (1 mentions) Plastipak, by BD (1 mentions) Pico plus elite, by Harvard Apparatus (1 mentions) Specord 200 plus, by Analytik Jena (1 mentions) Injekt f, by B. Braun (1 mentions)

9 protocols using omnifix f

Stable Water-in-Oil Droplet Production

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For stable production of water-in-oil
droplets,^{27 (link)−29 (link)} a 5 mM solution of PFPE (7000 g/mol)-PEG (1400 g/mol)-PFPE
(7000 g/mol) in HFE-7500 oil (3M, U.S.A.) was used as the oil phase.
The aqueous phase consisted of PBS with 2 μM, 20 nM, or 2 nM
Alexa Fluor 647 (C2-maleimide, A20347, Molecular Probes) as indicated.
Different droplet production frequencies, between ∼2–20
kHz, were generated by adjusting the flow rates of the aqueous and
oil phases ranging from 400 to 1000 μL/h and 800 to 3000 μL/h,
respectively (Supplementary Table S1).
All fluids were injected into the microfluidic device using 1 mL syringes
(Omnifix-F, B. Braun Melsungen AG, Germany) connected by a cannula
(Sterican, 0.4 × 20 mm, BL/LB, B. Braun) and PTFE-tubing (0.4
× 0.9 mm, Bola, Germany). For a fine flow-control, syringe pumps
(Pump11Elite, Harvard Apparatus, U.S.A.) were used. High-speed camera
(Phantom 7.2, Vision Research, U.S.A.) was used for a visual quality
assessment of droplets production.

Zamir E., Frey C., Weiss M., Antona S., Frohnmayer J.P., Janiesch J.W., Platzman I, & Spatz J.P. (2017). Reconceptualizing Fluorescence Correlation Spectroscopy for Monitoring and Analyzing Periodically Passing Objects. Analytical Chemistry, 89(21), 11672-11678.

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Syringe Residual Volume Evaluation

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We investigated three syringe models (Fig. 1): BD Plastipak 1 mL luer slip (Becton Dickinson S.A., Madrid, Spain), Omnifix-F 1 mL (B. Braun Medical Inc., Melsungen, Germany), and Zero Residual Enhanced Dosing Technology (EDT) 0.2 mL (SJJ Solutions BV, Den Haag, Netherlands). Notably, the last model is designed to deliver very small volumes accurately. The BD Plastipak and the Omnifix-F were tested with a standard 30-gauge BD PrecisionGlide needle (Becton Dickinson S.A., Curitiba, Brazil), whereas the Zero Residual EDT was assembled with a 30-gauge Zero Residual needle (SJJ Solutions BV, Den Haag, Netherlands).Fig. 1The syringes tested in the study.

Left: Omnifix-F from B. Braun Medical Inc. Middle: BD Plastipak from Becton Dickinson S.A. Right: Zero Residual EDT from SJJ Solutions BV.

A trained member of the research team prepared and assessed each syringe-needle setup. Distilled water was used as the injection fluid, and volumes of 10, 20, 25, and 50 µL were tested. The weight of the syringe-needle setup was measured before and after liquid aspiration and after ejection. The extra syringe-needle weight after liquid ejection defined the residual volume. Each simulation utilized a new syringe and needle. The weight was measured with an analytical balance (OHAUS Model PR224, Parsippany, NJ). The study was conducted at a stable 23 °C room temperature and 70% humidity.

Melo G.B., Santos L.A., Agra L.L., Meyer C., Moe M.C, & Jørstad Ø.K. (2023). Accuracy and residual volume of intravitreal injection syringes in administration of very small volumes. Eye, 38(2), 395-396.

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Microfluidic Water-in-Oil Droplet Formation

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To form stable water-in-oil droplets
at the flow-focusing T-junction
(Figure S1A),^{26 (link)−28 (link)} we used an
aqueous phase and an oil phase containing 3 wt % of perfluoropolyether–PEG
block copolymer fluorosurfactants (PEG-based fluorosurfactants from
Ran Biotechnologies, Inc., USA) dissolved in HFE 7500 oil (3M, USA).
The flow rates were set to 400 μL/h for both phases. The liquids
were injected with syringe pumps (11 PicoPlus Elite, Harvard Apparatus,
USA) into the device in 1 mL syringes (Omnifix-F, B. Braun, Germany)
connected by a cannula (Sterican, 0.4 × 20 mm², BL/LB,
B. Braun, Germany) and a PTFE tubing (0.4 × 0.9 mm, Bola, Germany).
Under these settings, droplets with a diameter of 60 μm were
produced at a rate of 1.3 kHz. A high-speed camera (Phantom 7.2, Vision
Research, USA) was used to assess the quality of the produced droplets.
The recorded videos were adjusted for brightness and contrast and
analyzed with ImageJ (NIH, USA).

Frey C., Göpfrich K., Pashapour S., Platzman I, & Spatz J.P. (2020). Electrocoalescence of Water-in-Oil Droplets with a Continuous Aqueous Phase: Implementation of Controlled Content Release. ACS Omega, 5(13), 7529-7536.

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Anaerobic Culture Inoculation and Sampling

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For inoculation, sampling and gassing of anaerobic cultures of M. maripaludis the following sterile equipment was utilized: 1 mL, 5 mL and 10 mL gas-tight syringes (Injekt®-F, Omnifix®-F, Omnifix®; B. Braun, Melsungen, Germany); hypodermic needles (Gr 14, 0.60 × 30 mm, 23 G × 1 1/4′′; B. Braun, Melsungen, Germany) and cellulose acetate filters with pore size 0.20 μm (LLG Labware, Meckenheim, Germany). A gassing manifold (Taubner and Rittmann, 2016 (link)) was used for feeding cultures. A digital manometer (LEO1-Ei, −1…3 bar rel, Keller, Germany) was used for pressure measurements. Serum bottles were shaken or stirred with a magnetic stir bar at 37°C at various rotations per minute (rpm). Assessment of culture growth was carried out by optical density measurements utilizing a spectrophotometer Specord 200 Plus (Analytic Jena, Jena, Germany) at 578 nm (OD₅₇₈).

Palabikyan H., Ruddyard A., Pomper L., Novak D., Reischl B, & Rittmann S.K. (2022). Scale-up of biomass production by Methanococcus maripaludis. Frontiers in Microbiology, 13, 1031131.

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Extracting and Imaging AirGels with Lightsheet Microscopy

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To perform lightsheet microscopy on AirGels, we needed to extract the ECM gel and cells from the PDMS chip. After fixation and staining, we filled in the lumen with a 1% low–melt agarose solution in order to ensure structural integrity of the airway. We let it solidify; using tweezers, we could then carefully detach the PDMS from the glass; indeed, since the surface of the 3D printed mold was not perfectly smooth, plasma bonding was not irreversible, which we could leverage for ECM extraction. We then used a scalpel and a spatula to release the piece of ECM from the PDMS chip and later embedded it in 1% low–melt agarose. While the agarose was still liquid, we aspirated the whole gel into a 1–ml syringe (Omnifix–F, B. Braun), whose tip had previously been cut out. After the agarose solidified, we could then use the plunger to freely push the fixed AirGel in and out of the syringe, in order to image it with selective plane illumination microscope (SPIM).

Rossy T., Distler T., Meirelles L.A., Pezoldt J., Kim J., Talà L., Bouklas N., Deplancke B, & Persat A. (2023). Pseudomonas aeruginosa type IV pili actively induce mucus contraction to form biofilms in tissue-engineered human airways. PLOS Biology, 21(8), e3002209.

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Bacterial Challenge Dose Enumeration

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To enumerate bacterial CFU for a prepared challenge dose, a 1 mL low dead-space (LDS) syringe (B-Braun Omnifix-F, Melsungen, Germany), was connected to a 36G drawing-up needle, and 200 μL volume of thawed JKD8049 filtrate was aspirated after vortexing for 5 seconds. 800 μL of PBS was drawn into a separate 1 mL LDS syringe, and the two syringes were connected via luer-lock connector (B-Braun Fluid Dispensing Connector, Melsungen, Germany). The bacterial solution and PBS were then mixed into one another 15 times to create a well-mixed suspension, pressing in a steady back-and-forth motion to compound the suspension (Fig 2). The solution was then plunged into a single syringe and the luer-lock connector was replaced by a 30G low dead-space hypodermic needle (TSK LDS-30013, Tochigi-Ken, Japan). From this final 1 mL sample, 0.1 mL replicates were injected directly onto 7H10 Middlebrook agar with OADC, creating 10 technical replicates.

Muhi S., Buultjens A.H., Porter J.L., Marshall J.L., Doerflinger M., Pidot S.J., O’Brien D.P., Johnson P.D., Lavender C.J., Globan M., McCarthy J., Osowicki J, & Stinear T.P. (2024). Mycobacterium ulcerans challenge strain selection for a Buruli ulcer controlled human infection model. PLOS Neglected Tropical Diseases, 18(5), e0011979.

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Shear Flow Dynamics of Differentiated HL-60 Cells

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Channel slides with dimensions of 50 × 5 × 0.2 mm² ((l:w:h), Ibidi GmbH, Mastinsried, Germany) were used to investigate differentiated HL-60 cell behavior under shear flow. The flow rate, controlled by syringe pumps (Pump11Elite, Harvard Apparaturs, USA), was kept at either 30 µL/hour or 40 µL/hour. Media was injected with 1 mL syringes (Omnifix®-F, B.Braun, Germany) connected by a cannula (Sterican®, 0.4 × 20 mm², BL/LB, B.Braun, Germany) and PTFE-tubing (0.4 × 0.9 mm², Bola, Germany). The cells were kept under constant flow for 1 h and imaged at 1 min intervals. For each of the two flow conditions, 2 independent experiments were performed. In total, 50 cells from at least 5 different chamber positions were tracked.

Khachaturyan G., Holle A.W., Ende K., Frey C., Schwederski H.A., Eiseler T., Paschke S., Micoulet A., Spatz J.P, & Kemkemer R. (2022). Temperature-sensitive migration dynamics in neutrophil-differentiated HL-60 cells. Scientific Reports, 12, 7053.

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Neural Therapy for Ear Scar in Cattle

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Neural therapy on the ABVN of the scar of the left ear was performed in TRE on d0, d2 and d4. For this purpose, each cow was restrained in the feeding fence and the head was tied to the right side of the animal using a halter. The whole ear, especially the area around the ear tag, was examined for pathological changes such as marked reddening, swelling, exudate and hyperalgesia by palpation. After clipping all long hair of the pinna, the area was cleaned and disinfected using 70% ethyl alcohol-soaked cotton balls (Ethanol 70%, Liquid Production GmbH, Flintsbach am Inn, Austria). Cows of TRE group were treated by intracutaneous infiltration of 0.1 ml procaine hydrochloride 2% (Procamidor® 20 mg/ml, Richterpharma AG, Wels, Austria) at each location clockwise at 12, 3, 6 and 9 around the ear tag (Fig. 2) using 1 ml syringes (Omnifix®-F, B. Braun Melsungen AG, Melsungen, Germany) and 0.6 × 30 mm needles (Henry Schein Inc. Melville, USA). Cows from CON group were handled identically, however, without injection of local anaesthetics.

Winkler M.J., Franz S., Wittek T, & Pothmann H. (2021). A potential treatment approach for subclinical mastitis in dairy cows: auriculotherapy of the auricular branch of the vagus nerve. The Journal of dairy research, 88(4).

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Flow Rate Measurements for Visualization

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Flow rate measurements were conducted at room temperature within a period of three days using de-ionized water supplemented with watercolor (Ecoline Liquid Watercolour, Royal Talens) for improved visualization. Luer connectors (BDMFTLL-9, Nordson MEDICAL) were connected to the inlet and outlet using 2 mm thick, laser cut (VLS2.30, Universal Laser Systems) PMMA (Oroglas cast acrylic glass, Trinseo) adapters equipped with adhesive tape (ARcare 90106, Adhesives Research). Syringes with volume scale (1 mL, Omnifix-F, B. Braun Melsungen) were attached to the luer connectors, filled with equal amounts of liquid. Using a 5 s ramp time to the respective, final motor speed, the time needed for changing the volume in both syringes (0.05 mL per measurement) was taken. The volume flow rate was averaged from in total two systems from independent discs, each system was measured three times.

Schneider S., Bubeck M., Rogal J., Weener H.J., Rojas C., Weiss M., Heymann M., van der Meer A.D, & Loskill P. (2021). Peristaltic on-chip pump for tunable media circulation and whole blood perfusion in PDMS-free organ-on-chip and Organ-Disc systems. Lab on a chip, 21(20).

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