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Cxcr4 biotin

Manufactured by R&D Systems
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CXCR4 Biotin is a recombinant human protein that is biotinylated. CXCR4 is a chemokine receptor that binds the chemokine CXCL12/SDF-1. This product can be used for research purposes.

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Lab products found in correlation

Apc cy7, by R&D Systems (2 mentions) Igg1 fitc, by Thermo Fisher Scientific (2 mentions) Cd138 bv421, by Thermo Fisher Scientific (2 mentions) Click it plus edu kit, by Thermo Fisher Scientific (2 mentions) Cd4 pe cy7, by Thermo Fisher Scientific (2 mentions) B220 apc cy7 or bv785, by R&D Systems (2 mentions) Igd fitc, by R&D Systems (2 mentions) Gl 7 pacific blue, by R&D Systems (2 mentions) Fas pe cy7, by R&D Systems (2 mentions) Cd38 alexafluor647, by R&D Systems (2 mentions) Cd45.2 bv605, by R&D Systems (2 mentions) Hvem pe, by R&D Systems (2 mentions) Btla alexafluor 647 8f4, by R&D Systems (2 mentions) Ephrin b1 biotin, by R&D Systems (2 mentions) Cd86 alexafluor647, by R&D Systems (2 mentions) Cd73 percp cy5, by Thermo Fisher Scientific (2 mentions) Tcrβ pacific blue, by Thermo Fisher Scientific (2 mentions) Cxcr5 bv605, by Thermo Fisher Scientific (2 mentions) Pd 1 pe or fitc, by Thermo Fisher Scientific (2 mentions) Cd40l pe, by Thermo Fisher Scientific (2 mentions)

Spelling variants of this product

CXCR4 (29 citations)

3 protocols using cxcr4 biotin

1

Multiparametric Flow Cytometry Analysis

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Cells were stained on ice, in 96 round bottom plates in PBS supplemented with 2% FBS and 1mM EDTA. The following antibodies were used: B220 APC-CY7 or BV785 (clone RA3-6B2), IgD FITC (clone 11-26c.2a), GL-7 Pacific Blue (clone GL7), FAS PE-CY7 (clone Jo2), CD38 AlexaFluor647 (clone 90), CD45.1 PERCP-CY5.5, APC (clone A20), CD45.2 BV605, APC-CY7 (clone 104), HVEM PE (clone LH1), BTLA AlexaFluor 647 (8F4), Ephrin-B1 Biotin (polyclonal, R&D), CXCR4 Biotin (clone 2B11/CXCR4), CD86 AlexaFluor647 (clone GL-1), IgG1 FITC (clone RMG1-1), CD138 BV421 (clone 281-2), CD73 PERCP-CY5.5 (clone TY/11.8), CD4 PE-CY7 (clone GK1.5), TCRβ Pacific Blue (clone H57-597), CXCR5 BV605 (clone L138D7), PD-1 PE or FITC (clone 29F.1A12), CD40L PE (clone MR1), Vα2 PERCP-CY5.5 (clone B20.1), Active caspase-3 (clone C92-605), Fixable viability dye eFluor780 (eBioscience). NP-47-PE (Biosearch technologies). For intracellular staining, cells were fixed and permeabilized using BD Cytofix/Cytoperm kit. EdU was detected with Click-IT Plus EdU kit (Invitrogen). Data were collected on a BD LSRII and analyzed on FlowJo Software.
Mintz M.A., Felce J.H., Chou M.Y., Mayya V., Xu Y., Shui J.W., An J., Li Z., Marson A., Okada T., Ware C.F., Kronenberg M., Dustin M.L, & Cyster J.G. (2019). The HVEM-BTLA Axis Restrains T Cell Help to Germinal Center B Cells and Functions as a Cell-Extrinsic Suppressor in Lymphomagenesis. Immunity, 51(2), 310-323.e7.
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2

Multiparameter Flow Cytometry for Immune Cell Analysis

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Cells were stained on ice, in 96 round bottom plates in PBS supplements with 2% FBS and 1mM EDTA. The following antibodies were used: B220 APC-CY7 or BV785 (clone RA3–6B2), IgD FITC (clone 11–26c.2a), GL-7 Pacific Blue (clone GL7), FAS PE-CY7 (clone Jo2), CD38 AlexaFluor647 (clone 90), CD45.1 PERCP-CY5.5, APC (clone A20), CD45.2 BV605, APC-CY7 (clone 104), HVEM PE (clone LH1), BTLA AlexaFluor 647 (8F4), Ephrin-B1 Biotin (polyclonal, R&D), CXCR4 Biotin (clone 2B11/CXCR4), CD86 AlexaFluor647 (clone GL-1), IgG1 FITC (clone RMG1–1), CD138 BV421 (clone 281–2), CD73 PERCP-CY5.5 (clone TY/11.8), CD4 PE-CY7 (clone GK1.5), TCRβ Pacific Blue (clone H57–597), CXCR5 BV605 (clone L138D7), PD-1 PE or FITC (clone 29F.1A12), CD40L PE (clone MR1), Vα2 PERCP-CY5.5 (clone B20.1), Active caspase-3 (clone C92–605), Fixable viability dye eFluor780 (eBioscience). NP-47-PE (Biosearch technologies). For intracellular staining, cells were fixed and permeabilized using BD Cytofix/Cytoperm kit. EdU detection was with Click-IT Plus EdU kit (Invitrogen). Data were collected on a BD LSRII and analyzed on FlowJo Software.
Mintz M.A., Felce J.H., Chou M.Y., Mayya V., Xu Y., Shui J.W., An J., Li Z., Marson A., Okada T., Ware C.F., Kronenberg M., Dustin M.L, & Cyster J.G. (2019). The HVEM-BTLA Axis Restrains T Cell Help to Germinal Center B Cells and Functions as a Cell-Extrinsic Suppressor in Lymphomagenesis. Immunity, 51(2), 310-323.e7.
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3

Cell Surface Marker Staining and Analysis

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For cell staining, cells were incubated (3 × 105 cells/well) with appropriate antibodies diluted in 50 µl staining buffer (PBS, 5% FCS, 1% BSA, 5 mM EDTA). After incubation (30 min, 4°C), cells were washed twice with staining buffer and analyzed in a Gallios cytometer (Beckman Coulter, CA, USA). Experiments were analyzed using FlowJo software (FlowJo, OR, USA).
Antibodies used include anti-mCD95-PE (BD Biosciences, CA, USA), -mCD95L-PE (BD Biosciences), and -CXCR4-biotin (R&D Systems, MN, USA). Streptavidin PE was from Beckman Coulter.
Rodríguez-Frade J.M., Guedán A., Lucas P., Martínez-Muñoz L., Villares R., Criado G., Balomenos D., Reyburn H.T, & Mellado M. (2017). Use of Lentiviral Particles As a Cell Membrane-Based mFasL Delivery System for In Vivo Treatment of Inflammatory Arthritis. Frontiers in Immunology, 8, 460.
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