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Peroxidase conjugated anti mouse or anti rabbit igg

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Peroxidase-conjugated anti-mouse or anti-rabbit IgG is a secondary antibody used in immunoassays and Western blotting techniques. It binds to the primary antibody and is conjugated to the enzyme peroxidase, which can be used to detect the target protein.

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Lab products found in correlation

Gapdh, by Abcam (1 mentions) Ecl reagent, by Thermo Fisher Scientific (1 mentions) Bca protein assay kit, by Thermo Fisher Scientific (1 mentions) Protease inhibitor cocktail tablet, by Roche (1 mentions) Protein assay kit, by Bio-Rad (1 mentions) Pvdf membrane, by Merck Group (1 mentions) Ecl western blot detection kit, by Merck Group (1 mentions) Chemidoc mp imager, by Bio-Rad (1 mentions) Ecl prime western blotting detection reagent, by Cytiva (1 mentions) Image lab, by Bio-Rad (1 mentions) Tricine sample buffer, by Bio-Rad (1 mentions) Protran nitrocellulose membrane, by Cytiva (1 mentions) Ecl prime reagent, by Cytiva (1 mentions) Gelsolin, by Abcam (1 mentions) β actin, by Merck Group (1 mentions) Lamin a c, by Santa Cruz Biotechnology (1 mentions) Whatman, by Cytiva (1 mentions) Protran, by Cytiva (1 mentions)

Spelling variants of this product

IgG Rabbit (4 citations) Mouse anti-IgG (2 citations)

4 protocols using peroxidase conjugated anti mouse or anti rabbit igg

1

Western Blot Analysis of ST8SIA4 in Cholangiocarcinoma

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Total proteins were extracted from cholangiocarcinoma tissues and cells, and protein concentrations were measured using the BCA protein assay kit (Thermo Fisher Scientific). Protein extracts were separated on 10% SDS-PAGE gels and subsequently transferred to PVDF membranes. The membranes were incubated with antibodies against GAPDH (1: 10000; Abcam, UK) and ST8SIA4 (1:1000; ABclonal, USA) at 4°C overnight. Peroxidase-conjugated anti-mouse or anti-rabbit IgG (1: 5000; Thermo Fisher Scientific) was used as the secondary antibody, and the bands were visualized using ECL reagent (Thermo Fisher Scientific). ST8SIA4 was normalized to GAPDH levels upon band intensity quantification through the Image Lab software.
Fu W., Yu G., Liang J., Fan P., Dong K., Zhang B., Chen X., Zhu H, & Chu L. (2021). miR-144-5p and miR-451a Inhibit the Growth of Cholangiocarcinoma Cells Through Decreasing the Expression of ST8SIA4. Frontiers in Oncology, 10, 563486.
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2

Western Blot Protocol for Protein Quantification

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For each sample, 1×106 cells were lysed using a solubilizing solution [20 mM Tris-HCl (pH 7.4), 150 mM NaCl, 1% NP-40, 1 mM PMSF, 0.02% NaN3, protease inhibitor cocktail tablet; Roche, Mannheim, Germany]. Protein concentration was determined using a Bio-Rad Protein assay kit (Hercules, CA, USA). An equal quantity (10–30 μg) of proteins was separated by 10–15% SDS-PAGE and transferred onto a PVDF membrane (Millipore Corp., Billerica, MA, USA). The membrane was blocked in 10% skim milk (in TBS, pH 7.2, containing 0.1% Tween-20) overnight at 4°C, then incubated with primary antibodies followed by peroxidase-conjugated anti-mouse or anti-rabbit IgG (Thermo Fisher, Inc., Rockford, IL, USA). The epitope was detected using an ECL western blot detection kit (Millipore Corp.). GADPH was used as an internal control for all western blots.
HUANG Y., YANG X., XU T., KONG Q., ZHANG Y., SHEN Y., WEI Y., WANG G, & CHANG K.J. (2016). Overcoming resistance to TRAIL-induced apoptosis in solid tumor cells by simultaneously targeting death receptors, c-FLIP and IAPs. International Journal of Oncology, 49(1), 153-163.
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3

Mitochondrial Complex Protein Detection

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Immunoblotting was performed with primary antibodies raised against human GSN, complex I NDUFA9 subunit, complex II SDHA subunit, complex III CORE2 subunit (Abcam, Cambridge, UK), complex IV COX5B subunit (Santa Clara, TX, USA), or μ-Actin (Sigma). Peroxidase-conjugated anti-mouse or anti-rabbit IgGs (Molecular Probes) were used as secondary antibodies. Immunoreactive bands were detected with ECL Prime Western Blotting Detection Reagent (Amersham Biosciences, UK) in a ChemiDoc™ MP Imager (Bio-Rad), and their optical densities were measured using the ImageLab™ (Bio-Rad) and the ImageJ analysis software.
García-Bartolomé A., Peñas A., Illescas M., Bermejo V., López-Calcerrada S., Pérez-Pérez R., Marín-Buera L., Domínguez-González C., Arenas J., Martín M.A, & Ugalde C. (2020). Altered Expression Ratio of Actin-Binding Gelsolin Isoforms Is a Novel Hallmark of Mitochondrial OXPHOS Dysfunction. Cells, 9(9), 1922.
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4

Western Blot Analysis of Protein Expression

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The supernatants (corresponding to 10 μg of whole cell extracts) were mixed with an equal volume of tricine sample buffer (Biorad) containing 2% (v/v) 2-mercaptoethanol. The mixtures were boiled for 5 min and proteins were separated on standard 10% SDS-PAGE gels and transferred onto PROTRAN™ nitrocellulose membranes (Whatman®). Membranes were blocked in phosphate-buffered saline (pH 7.4) containing 0.1% Tween-20 and 5% non-fat dried milk for at least 2 hours at room temperature. The blots were then hybridized with antibodies raised against the following proteins: lamin-A/C (Santa Cruz), gelsolin (Abcam), glyceraldehyde 3 phosphate dehydrogenase (Mitosciences), DJ-1 (Abcam) and β-actin (Sigma). Peroxidase-conjugated anti-mouse or anti-rabbit IgGs (Molecular Probes) were used as secondary antibodies. Immunoreactive bands were detected by chemiluminescence with ECL® prime reagents (Amersham Biosciences) in a G:Box Chemi IR6 Image Analyzer (Syngene). Quantitative changes in band intensities were evaluated by densitometric scanning with the Image J software and the densitometric values were normalized to those of the loading controls.
Marín-Buera L., García-Bartolomé A., Morán M., López-Bernardo E., Cadenas S., Hidalgo B., Sánchez R., Seneca S., Arenas J., Martín M.A, & Ugalde C. (2014). Differential proteomic profiling unveils new molecular mechanisms associated with mitochondrial complex III deficiency. Journal of proteomics, 113, 38-56.
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