α1 3 6 galactosidase

Manufactured by New England Biolabs

α1-3,6 galactosidase is an enzyme that catalyzes the hydrolysis of terminal non-reducing α-D-galactose residues in α-D-galactosides. It is a useful tool for the study and analysis of glycosidic linkages.

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Lab products found in correlation

Bovine fetuin, by Merck Group (1 mentions) α1 2 3 mannosidase, by New England Biolabs (1 mentions) α2 3 6 8 neuraminidase, by New England Biolabs (1 mentions) Formic acid, by Merck Group (1 mentions) Iodoacetamide, by Merck Group (1 mentions) Acetonitrile, by Merck Group (1 mentions) Dithiothreitol (dtt), by Merck Group (1 mentions) Ammonium bicarbonate, by Merck Group (1 mentions) Pngase f, by New England Biolabs (1 mentions) Maxq 4000, by Thermo Fisher Scientific (1 mentions)

2 protocols using α1 3 6 galactosidase

Glycoprotein Characterization by PGC-LC-MS

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Acetonitrile (ACN), ammonium bicarbonate (NH₄HCO₃), dithiothreitol (DTT), iodoacetamide, formic acid (FA) and bovine fetuin (Catalog no. F2379) were obtained from Sigma-Aldrich. PNGase F, α1-2,3 mannosidase, α1-3,6 galactosidase and α2-3,6,8 neuraminidase were purchased from New England Biolabs. LacZ β-galactosidase was purchase from Roche Diagnostics. mAbs were obtained from pharmaceutical companies.
Micro PGC column materials were obtained from Mandel Scientific Corporation, and capillary Hypercarb PGC materials (particle size 3 µm, pore size 250 Å) were obtained from Thermo Fisher Scientific. Fused silica capillary tubing was purchased from Polymicro Technologies Inc. Capillary PGC columns were packed in-house as reported previously^{32 (link)}.

She Y.M., Dai S, & Tam R.Y. (2022). Highly sensitive characterization of non-human glycan structures of monoclonal antibody drugs utilizing tandem mass spectrometry. Scientific Reports, 12, 15109.

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Decellularization of Osteochondral Plugs

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Cylindrical osteochondral plugs, 5 mm diameter × ~8 mm, were harvested from the femoral condyles and patellofemoral grooves. Cleaning and decellularization was accomplished by incubating plugs in the following solutions (20 plugs in 50 ml of solution per batch):
All steps were carried out under orbital shaking at 37 °C (MaxQ 4000, Thermo Scientific, Marietta, OH), except for steps 1, 8, and 9, which were carried out at room temperature. For the α-Gal immunohistochemistry experiment, some tissue underwent an additional processing step after the SDS treatment. Those plugs were incubated for 4 hours at 37 °C in 25U/ml α1–3,6 galactosidase (New England BioLabs, Ipswich, MA), 50 mM sodium acetate (pH 5.5), 5 mM CaCl₂, and 0.01% wt/vol bovine serum albumin (BSA).

Elder S., Chenault H., Gloth P., Webb K., Recinos R., Wright E., Moran D., Butler J., Borazjani A, & Cooley A. (2018). Effects of Antigen Removal on a Porcine Osteochondral Xenograft for Articular Cartilage Repair. Journal of biomedical materials research. Part A, 106(8), 2251-2260.

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