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Mouse ccl11 eotaxin quantikine elisa kit

Manufactured by R&D Systems
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The Mouse CCL11/Eotaxin Quantikine ELISA Kit is a quantitative sandwich enzyme immunoassay designed to measure mouse CCL11/Eotaxin levels in cell culture supernates, serum, and plasma.

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Lab products found in correlation

Myhcα614 62g ac slklmatlfstyasad, by GenScript (2 mentions) Il 25, by R&D Systems (1 mentions) Il 33, by BioLegend (1 mentions)

Close spelling variants of this product

CCL11/eotaxin Quantikine ELISA kit Quantikine ELISA CCL11 Eotaxin Mouse ELISA kits Quantikine kits Quantikines ELISAs

5 protocols using mouse ccl11 eotaxin quantikine elisa kit

1

Cardiac Fibroblast-ILC2 Co-culture ELISA

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Supernatants from cardiac fibroblasts co-culture with ILC2s or sera from mice were stored at −80°C prior to ELISA. Eotaxin-1 was determined by quantitative sandwich ELISA using Mouse CCL11/Eotaxin Quantikine ELISA Kit (R&D Systems) according to manufacturer’s protocols. For anti-myosin IgM ELISA, plates were coated with 0.5 μg/well myosin heavy chain α peptide MyHCα614-62g (Ac-SLKLMATLFSTYASAD; Genscript) and Alkaline Phosphatase AffiniPure goat anti-mouse IgM, μ chain specific (Jackson ImmunoResearch) at a 1:4000 dilution was used to detect anti-myosin IgM in sera.
Choi H.S., Won T., Hou X., Chen G., Bracamonte-Baran W., Talor M.V., Jurčová I., Szárszoi O., Čurnova L., Stříž I., Hooper J.E., Melenovský V, & Čiháková D. (2020). Innate Lymphoid Cells Play a Pathogenic Role in Pericarditis. Cell reports, 30(9), 2989-3003.e6.
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2

Characterizing Murine Type 2 Immunity

Cited 3 times
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IL5Tg (5 (link)), Arg1YFP (42 (link)), Il5Red5Cre (Red5) (10 (link)), ILC2-deleted (ILC2del) (10 (link)); STAT6–/– (43 (link)), Smart13 (35 (link)), IL4Rα–/– (44 (link)), and IL4–/–/IL13–/– (45 (link)) mice have been described. Mice were derived or backcrossed on a C57BL/6J background for more than 10 generations, and housed under specific pathogen–free conditions with 12-hr light/day cycles and ad libitum access to food and water. Experiments were performed on age- and sex-matched male and female mice between 8 weeks and 16 weeks of age, except for the eotaxin experiments, in which mice were 6 months of age. IL-33 (BioLegend or R&D), TSLP (eBioscience), or IL-25 (R&D) were administered intranasally at 0.5 μg per dose, 3 times a week for 2 weeks, and mice were analyzed 1 day after the final dose. For intravascular labeling experiments, mice were injected intravenously with 3 μg of anti-mouse CD45 Ab (clone 30-F11) 3 minutes prior to sacrifice. Eotaxin was measured from serum using the Mouse CCL11/Eotaxin Quantikine ELISA Kit (R&D).
Kotas M.E., Dion J., Van Dyken S., Ricardo-Gonzalez R.R., Danel C.J., Taillé C., Mouthon L., Locksley R.M, & Terrier B. (2021). A role for IL-33–activated ILC2s in eosinophilic vasculitis. JCI Insight, 6(12), e143366.
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3

Plasma CCL11 ELISA Quantification

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Non-fasting plasma samples were used to evaluate plasma CCL11 levels using a commercially available Mouse CCL11/Eotaxin Quantikine ELISA Kit (R&D Systems, Minneapolis, MN) according to the manufacturer’s instruction. Plasma CCL11 levels were measured in both CT and EX groups at baseline and at the end of the 8-week study.
Lezi E., Burns J.M, & Swerdlow R.H. (2014). Effect of high-intensity exercise on aged mouse brain mitochondria, neurogenesis, and inflammation. Neurobiology of aging, 35(11), 2574-2583.
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4

Quantifying Chemokines in Cell Supernatants

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Cell culture supernatants were assayed for CCL11 using the Mouse CCL11/Eotaxin Quantikine ELISA Kit (R&D systems) and for CCL24 using the Eotaxin-2 (CCL24) Mouse ELISA Kit (Abcam).
Diny N.L., Hou X., Barin J.G., Chen G., Talor M.V., Schaub J., Russell S.D., Klingel K., Rose N.R, & Čiháková D. (2016). Macrophages and cardiac fibroblasts are the main producers of eotaxins and regulate eosinophil trafficking to the heart. European journal of immunology, 46(12), 2749-2760.
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5

Cardiac Fibroblast-ILC2 Co-culture ELISA

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Supernatants from cardiac fibroblasts co-culture with ILC2s or sera from mice were stored at −80°C prior to ELISA. Eotaxin-1 was determined by quantitative sandwich ELISA using Mouse CCL11/Eotaxin Quantikine ELISA Kit (R&D Systems) according to manufacturer’s protocols. For anti-myosin IgM ELISA, plates were coated with 0.5 μg/well myosin heavy chain α peptide MyHCα614-62g (Ac-SLKLMATLFSTYASAD; Genscript) and Alkaline Phosphatase AffiniPure goat anti-mouse IgM, μ chain specific (Jackson ImmunoResearch) at a 1:4000 dilution was used to detect anti-myosin IgM in sera.
Choi H.S., Won T., Hou X., Chen G., Bracamonte-Baran W., Talor M.V., Jurčová I., Szárszoi O., Čurnova L., Stříž I., Hooper J.E., Melenovský V, & Čiháková D. (2020). Innate Lymphoid Cells Play a Pathogenic Role in Pericarditis. Cell reports, 30(9), 2989-3003.e6.
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