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High precision analytical balance

Manufactured by Mettler Toledo
Sourced in Germany

The High Precision Analytical Balance is a laboratory instrument designed to provide highly accurate measurements of small masses. It is capable of weighing samples with exceptional precision, typically in the microgram range. The balance's core function is to enable accurate and reproducible weighing of various materials and substances in a laboratory setting.

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5 protocols using high precision analytical balance

1

Scaffold Swelling Kinetics in Phosphate Buffer

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The scaffolds were cut into sections with about 10 mm thickness and 10 mm diameter. The dry weight of specimens was measured by a high precision analytical balance (Mettler Toledo, Germany). Then, specimens were immersed in buffered phosphate solution (PBS) and incubated at 37 °C. The wet weight and dimensions of the specimens was measured at different time intervals for 24 h. Before weighing, the surface water of the specimens was removed by a filter paper. The scaffold swelling percentage was calculated using following formula: Swelling%=Ww-WdWd×100 , which Ww and Wd are wet weight and dry weight, respectively. All tests were performed in triplicate and the mean values were reported.
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2

Automated Determination of 88Sr by ICP-MS

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Sr resin cartridges (2 mL) with a vacuum box for 24 samples were purchased from Eichrom Technology while Sr resin columns (CF-MC-Sr-S-2000) with the 1-probe/4-columns prepFast MC computer-controlled system and DGA (SF-MC-SrCa-2000) columns with the 5-probe/5-columns prepFast MC computer-controlled system were purchased from ESI. The ultra-low-level liquid scintillation spectrometer Quantulus1220 and 20-mL plastic LSC vials (all from PerkinElmer) were used for LSC analysis. For Sr-88 ICP-MS analysis (as Sr recovery method), a NexION®300 inductively coupled plasma dynamic reaction cell mass spectrometer (ICP-DRC-MS) (PerkinElmer) was used. Additional supplies included 15-mL and 50-mL conical polypropylene tubes from Beckton Dickinson Labware, a high precision analytical balance capable of accuracy to 0.0001 g (Mettler Toledo), a Brinkman bottle top dispenser with capacity from 5mL to 25mL (Brinkman Instruments) for LSC cocktail dispensing, and a set of four automatic pipettes with total volume range from 5 μL to 5 mL (Eppendorf).
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3

Determination of Sr-90 and Sr-88 in Environmental Samples

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For our analysis, we used Sr resin cartridges (2 mL) acquired over several years (see Table 2) and a vacuum box that can accommodate 24 samples (Eichrom Technologies). Three Quantulus1220 ultra-low-level liquid scintillation counters (PerkinElmer) were used for LSC Sr-90 analysis in alpha/beta mode and a NexION® 300 inductively coupled plasma dynamic reaction cell mass spectrometer (ICP-DRC-MS) (PerkinElmer) was used for Sr-88 ICP-MS analysis as a recovery method. We also used a high-precision analytical balance accurate to 0.0001 g (Mettler Toledo) and a hot plate (Fisher Scientific) for drying eluted samples after purification for gravimetric recovery. Additional supplies included 20 mL LSC plastic vials (PerkinElmer, Inc.); 15 mL and 50 mL conical polypropylene tubes (Beckton Dickinson Labware) for solution preparation, a bottle top dispenser with capacity from 5 mL to 25 mL (Brinkman Instruments) for LSC cocktail dispensing, and a set of four pipettes with total volume range from 5 μL to 5 mL (Eppendorf).
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4

Radioactive Isotope Measurement Protocols

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For this study, we used ultralow level liquid scintillation spectrometers—Quantulus1220, Tri-Carb®3110 (#1 and #2), Tri-Carb®5110, and Quantulus GCT6220 (total 5 instruments, all from Revvity Health Sciences, Inc) for Am-241, Sr-90/Y-90, and H-3, in alpha/beta mode; 20 mL LSC plastic vials (Revvity Health Sciences, Inc.) for LSC analysis; a high precision analytical balance capable of accuracy weighing 0.001 g (Mettler-Toledo, LLC); a bottle top dispenser with a 5 mL to 25 mL capacity (Brinkman Instruments, Inc.) for cocktail dispensing; 250 mL and 125 mL polypropylene bottles (Nalgene, Fisher Scientific) for urine spikes preparation; and four electronic pipettes with total volume ranges from 5 μL to 5 mL (Eppendorf, Inc).
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5

Versatile Radioisotope Analysis Protocol

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For this study we used two ultralow level liquid scintillation spectrometers Quantulus1220 (#2 and #3) and two Tri-Carb3110 (#1 and #2) (all from PerkinElmer Company) for Am-241, Sr-90/Y-90, H-3, and P-32 analysis in alpha/beta mode; 20-mL LSC plastic vials (PerkinElmer Company) for LSC analysis; a high precision analytical balance capable of accuracy weighing 0.0001 gm (Mettler-Toledo, LLC); 15-mL and 50-mL conical polypropylene tubes (Becton Dickinson Company) for solution preparation; a Brinkman bottletop dispenser with capacity from 5 mL to 25 mL (Brinkman Instruments, Inc.) for cocktail dispensing; and four electronic pipettes with total volume range from 5 μL to 5 mL (Eppendorf, Inc).
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