Samples containing bacterial cells in PBS were incubated with conjugates at 30 °C (R. solanacearum) or 37 °C (rest) at concentration of 1 mg/ml. Controls containing bacterial cells in PBS were incubated without conjugates at the same temperature. Subsequently, all samples were centrifuged at 7,300× g for 1 min, and washed with PBS twice followed by centrifugation. A drop containing the bacteria was absorbed onto a copper grid and negatively stained with phosphotungstic acid for 30 s. The grids were examined using a JEM-100CX-II electron microscope (JEOL Ltd., Japan)17 (link).
Jem 100cx 2 electron microscope
Manufactured by JEOL
Sourced in Japan, United States
The JEM-100CX II is a transmission electron microscope (TEM) manufactured by JEOL. It is designed to perform high-resolution imaging and analysis of materials at the nanoscale level. The JEM-100CX II is capable of magnifications up to 600,000x and can achieve a resolution of 0.34 nanometers.
Automatically generated - may contain errors
Lab products found in correlation
Ultracut microtome, by Leica (2 mentions)
Escalab 250 spectrometer, by Thermo Fisher Scientific (2 mentions)
Ultratome, by Leica (2 mentions)
Tg 209 f3 thermogravimetric analyzer, by Netzsch (1 mentions)
Rpmi medium, by Merck Group (1 mentions)
Zetasizer nano zs instrument, by Malvern Panalytical (1 mentions)
2010 electron microscope, by JEOL (1 mentions)
Nicolet is10, by Thermo Fisher Scientific (1 mentions)
Uv 2450, by Shimadzu (1 mentions)
Cf300 cu, by Electron Microscopy Sciences (1 mentions)
Seb705mu, by Cloud-Clone (1 mentions)
Himac cp 100wx, by Hitachi (1 mentions)
Lx 112 medium, by Ladd Research Industries (1 mentions)
Aqueous uranyl acetate, by Merck Group (1 mentions)
Lead citrate, by Serva Electrophoresis (1 mentions)
Sodium cacodylate buffer, by Serva Electrophoresis (1 mentions)
Toluidine blue, by Merck Group (1 mentions)
Spurr kit, by Merck Group (1 mentions)
Osmium tetroxide, by Serva Electrophoresis (1 mentions)
Glutaraldehyde, by Merck Group (1 mentions)
27 protocols using jem 100cx 2 electron microscope
1
Bacterial Visualization via Electron Microscopy
2
Characterization of Nanoparticle Properties
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Transmission electron microscopy (TEM) images of the nanoparticles were taken on a JEM-100CXII electron microscope (JEOL, Japan) at an acceleration voltage of 100 kV. The particle size was determined by averaging at least 300 particles per sample. Powder X-ray diffraction (XRD) patterns of the particle samples were recorded on a D/Max-2500 diffractometer (Regaku, Japan) under Cu Kα1 radiation (λ = 1.54056 Å). TGA measurements were performed on a TG209F3 thermogravimetric analyzer (NETZSCH, Germany). The magnetic properties of the resultant samples were characterized by using a vibrating sample magnetometer (VSM JDM-13, Changchun Yingpu Magnetoelectric Technology Development Co., Ltd., China). Dynamic light scattering (DLS) measurements were carried out at 298.0 K with a Nano ZS (Malvern, United Kingdom) equipped with a solid-state He-Ne laser (λ = 633 nm) for measuring the hydrodynamic size of the resultant nanoparticles. The concentration of Fe was determined by using an inductively coupled plasma atomic emission spectrometer (ICP-2000) produced by Jiangsu Skyray Instrument Co., Ltd. (China)
3
Interaction of T. rubrum with Macrophages
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The interaction of T. rubrum LGC with human macrophages was analyzed by electron microscopy (JEOL JEM 100CXII electron microscope). Initially, we performed the induction of the cells for their transformation into macrophages with PMA, and then we performed the co-culture with live T. rubrum conidia according to [20 (link)] which used 6-well plates and was then incubated for 24 h. The electron microscopy test was carried out according to [28 (link)].
4
T. rubrum Keratinocyte Co-Culture Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For co-culture assay, a ratio of 2.5 × 105 cells/mL of keratinocytes to 1 × 107 conidia/mL of T. rubrum solution was used, and the co-culture was performed as described in [13 (link)]. The assays were carried out in three independent experiments performed in triplicate. Cultured keratinocytes and T. rubrum conidia were used as controls and were cultured similarly to the co-infection in RPMI Medium (Sigma Aldrich). Scanning electron microscopy was performed with a JEOL JEM 100CXII electron microscope at the Multiuser Electron Microscopy Laboratory of the Department of Cell and Molecular Biology (Ribeirão Preto Medical School, São Paulo, Brazil) to determine whether the penetration of fungal hyphae into keratinocytes occurred within 24 h of co-culture. The cell viability of HaCat keratinocytes prior to T. rubrum inoculation and after 24 h of co-culture was determined by measuring the release of the enzyme lactate dehydrogenase (LDH) (TOX7 kit from Sigma-Aldrich) in the RPMI Medium (Sigma Aldrich) according to the manufacturer’s instructions and described in [14 (link)]. The absorbance was read in a microplate reader (Elx 800 UV Bio-Tek Instruments, Inc., Winooski, VT, USA) at 490 nm.
5
Spectroscopic Characterization of IL-CDs
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The high-resolution transmission electron microscopy (HRTEM) images of IL-CDs in both absence and presence of TBA were recorded using a JEOL-2010 electron microscope (Japan) operating at 200 kV. For negative-staining transmission electron microscopy (NS-TEM) observations, samples were placed on a carbon-coated copper grid and then stained with 1% uranyl acetate, and further observed on a JEM-100 CXII electron microscope (JEOL, Japan) at an accelerating voltage of 120 kV. UV-vis absorption spectra of the obtained IL-CDs were recorded on a UV-vis spectrophotometer (UV-2450, Shimadzu, Japan). The Fourier transform infrared (FT-IR) spectra of IL-CDs were recorded in the range of 500–4000 cm−1 using a NICOLET iS10 (Thermo Fisher, America) spectrometer. The X-ray diffraction (XRD) measurements of IL-CDs were performed on a Rotating Anode X-ray Powder Diffractometer (D/max-2550VB/PC, Rigaku) in the absence and presence of AOT. The X-ray photoelectron spectra (XPS) of the IL-CDs were performed on an ESCALAB 250 spectrometer (Thermo Fisher, America). Zeta potential of IL-CDs in solutions with and without the presence of AOT was measured to show the surface charge by a Malvern Zetasizer NanoZS instrument (Southborough, MA) equipped with a laser Doppler velocimeter using a folded capillary cell with a gold electrode.
6
Liposomal Nanoparticle Characterization by TEM
7
Antimicrobial Effects of α-Defensin-1
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
109 CFU of A. xylosoxidans were treated with 500 pg of recombinant α-defensin-1 peptide standard from ELISA kit (SEB705Mu—Cloud-Clone Corp. Houston, TX, USA), in 0.5 ml of PBS 1x containing 1% (v/v) BHI for 2 h at 37 °C. Next steps were performed as previously described50 (link). Bacteria were examined with JEM-100CX II electron microscope (JEOL, Peabody, MA, USA).
8
Visualizing Autophagy in MCF-7 Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
TEM is considered the most reliable approach to monitor autophagy (32 (link)). MCF-7 cells were cultured in 6-well plates for 24 h and infected with recombinant adenoviruses (100 MOI) for 6 h. Cells were then collected and cells were fixed in 2.5% glutaraldehyde at 4°C for 8 h. Ultrathin sections (50 nm) were cut using an ultramicrotome, stained with 2% (w/v) uranyl acetate for 15 min and with lead citrate for 10 min at room temperature, and examined under a JEM-100cxII electron microscope (JEM-1010; JEOL, Ltd., Tokyo, Japan).
9
Characterization of PEG-coated ZnO-NPs
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
A small amount of the PEG-coated in situ prepared ZnO-NPs powder was suspended in distilled water and sonicated for 20 hrs to ensure well dispersion. A drop of the previous solution was taken on a carbon-coated copper grid (400 mesh) for TEM imaging (JEOL JEM-100CX II Electron Microscope, Japan).
10
Electron Microscopy Visualization of Virus
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Electron microscopy (EM) was performed to observe the virus based on previous studies (Wang et al., 2021 (link)). For this, the allantoic membrane tissue fluid was first centrifuged at 7,000 g for 30 min at 4°C, and then, ultracentrifuged at 110,000 g for 2 h at 4°C (Hitachi Koki Himac CP 100WX, Japan). The pellets were then resuspended in PBS (pH 7.4) and loaded over a preformed 10–60% sucrose gradient, after which the sucrose gradient was centrifuged at 110,000 g for 1 h at 4°C. The purified virus pellets were resuspended in sterile 1× PBS (pH 7.4) buffer and then negatively stained with 2% phosphotungstic acid. The grids were observed under a JEM-100 CX-II electron microscope after blotting and drying (JEOLLTD, Japan).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!