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Focus v 3

Manufactured by Helicon

The Focus v. 3.10.3 is a laboratory equipment product. It provides core functionality for use in laboratory settings. No further details are available.

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5 protocols using focus v 3

1

Comprehensive Spider Specimen Examination Protocol

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Specimens were examined and measured with a Leica M205 C stereomicroscope. Left male palps and epigynes were photographed. Vulvae were treated in a warm 10% potassium hydroxide (KOH) solution to dissolve soft tissues before illustration. Images were captured with a Canon EOS 750D wide zoom digital camera (24.2 megapixels) mounted on the stereomicroscope mentioned above, and assembled using Helicon Focus v. 3.10.3 image-stacking software (Khmelik et al. 2005 ). All measurements are given in millimetres (mm). Leg measurements are shown as: total length (femur, patella, tibia, metatarsus, tarsus). Leg segments were measured on their dorsal side. The species distribution map was generated with ArcGIS v. 10.2 (ESRI Inc.). Type specimens are deposited in the Institute of Zoology, Chinese Academy of Sciences (IZCAS) in Beijing, China and the Vietnam National Museum of Nature (VNMN) in Hanoi, Vietnam.
Terminology and taxonomic descriptions follow Zhao and Peng (2014) (link), Jin et al. (2015) (link), and Liu et al. (2020) (link).
The following abbreviations are used in the descriptions:
AER anterior eye row;
ALE anterior lateral eye;
AME anterior median eye;
MOA median ocular area;
PER posterior eye row;
PLE posterior lateral eye;
PME posterior median eye;
RTA retrolateral tibial apophysis.
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2

Stereomicroscopic Examination and Imaging of Spider Specimen

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Specimens were examined and measured with a Leica M205 C stereomicroscope. The left male palp was photographed. The epigyne was photographed before dissection. The vulva was photographed after treating it in a 10% warm solution of potassium hydroxide (KOH) to dissolve soft tissues. Images were captured with a Canon EOS 750D wide zoom digital camera (24.2 megapixels) mounted on the stereomicroscope mentioned above and assembled using Helicon Focus v. 3.10.3 image-stacking software (Khmelik et al. 2005 ). All measurements are given in millimeters (mm). Leg measurements are shown as: total length (femur, patella, tibia, metatarsus, tarsus). Leg segments were measured on their dorsal side. The distribution map was generated with ArcGIS v. 10.2 (ESRI Inc.). The specimens studied are preserved in 75% ethanol and deposited in the
College of Life Science, Shenyang Normal University (SYNU) in Liaoning, China.
Terminology and taxonomic descriptions follow Huber (2011) and Yao et al. (2015 (link), 2021 (link)). The following abbreviations are used in the descriptions:
ALE = anterior lateral eye,
AME = anterior median eye,
PME = posterior median eye,
L/d = length / diameter ratio; used in the illustrations:
aa = anterior arch,
b = bulb,
da = distal apophysis,
e = embolus,
fa = frontal apophysis,
pa = proximo-lateral apophysis,
pp = pore plate,
pr = procursus,
u = uncus.
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3

Detailed Spider Morphology Documentation

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Specimens were examined and measured with a Leica M205 C stereomicroscope. The left male palp was photographed. The epigyne was photographed before dissection. The vulva was treated in a 10% warm solution of potassium hydroxide (KOH) to dissolve soft tissues before illustration. Images were captured with a Canon EOS 750D wide zoom digital camera (24.2 megapixels) mounted on the stereomicroscope mentioned above and assembled using Helicon Focus v. 3.10.3 image stacking software (Khmelik et al. 2005 ). All measurements are given in millimeters (mm). Leg measurements are shown as: total length (femur, patella, tibia, metatarsus, tarsus). Leg segments were measured on their dorsal side. The distribution map was generated with ArcGIS v. 10.2 (ESRI Inc.). The specimens studied are preserved in 75% ethanol and deposited in the
College of Life Science, Shenyang Normal University (SYNU) in Liaoning, China.
Terminology and taxonomic descriptions follow Huber (2011b) and Yao et al. (2015 (link), 2021 (link)). The following abbreviations are used in the descriptions:
ALE = anterior lateral eye,
AME = anterior median eye,
PME = posterior median eye,
L/d = length/diameter ratio; used in the illustrations:
b = bulb,
da = distal apophysis,
e = embolus,
fa = frontal apophysis,
pa = proximo-lateral apophysis,
pp = pore plate,
pr = procursus,
u = uncus.
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4

Spider Specimen Examination Protocol

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Specimens were examined and measured under a Leica M205 C stereomicroscope. Further details were examined using an Olympus BX51 compound microscope. Male palps and epigynes were examined and photographed after dissection. They were treated in lactic acid for several minutes, and subsequently embedded in Hoyer’s Solution before photographing. Photos were made with a Canon EOS 60D wide zoom digital camera (8.5 megapixels) mounted on the Olympus BX51 compound microscope. Images were combined using Helicon Focus v.3.10 software (Khmelik et al. 2006 ). All measurements are in millimetres. Leg measurements are given as follows: total length (femur, patella, tibia, metatarsus, and tarsus). Abbreviations of institutions and morphological terminology are given in Table 3. References to figures in cited papers are listed in lowercase (fig. or figs), and figures in this paper are noted with an initial capital (Fig. or Figs).
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5

Detailed Protocol for Analyzing Anapistula Specimens

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Specimens were studied in ethanol using a Leica M205 C stereomicroscope. Habitus and copulatory organs were photographed with a Canon EOS 60D wide zoom digital camera (8.5 megapixels) mounted on an Olympus BX 51 compound microscope. Male palps and epigynes were examined after dissection and treated with lactic acid before being embedded in Hoyer’s Gum and placed on an ultra-thin slide to take photos of the dorsal and ventral sides. The digital photos were montaged using Helicon Focus v.3.10 (Khmelik et al. 2006 ) image stacking software. All measurements are in millimetres. Leg measurements are given as follows: total length (femur, patella, tibia, metatarsus, tarsus).
Nomenclature of the genital structures was based on Dupérré and Tapia (2017) (link) and Rivera-Quiroz et al. (2021) (link) for Anapistula. Abbreviations in the text and figures are as follows:
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