Anti meh

Manufactured by Santa Cruz Biotechnology

Sourced in United States

Anti-mEH is a laboratory reagent used in research applications. It functions as an antibody that specifically binds to and detects the microsomal epoxide hydrolase (mEH) protein in various biological samples.

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Lab products found in correlation

Anti β actin, by Merck Group (1 mentions) Pvdf membrane, by Merck Group (1 mentions) Cdp star reagent, by Roche (1 mentions) Horseradish peroxidase conjugated anti rabbit igg, by Merck Group (1 mentions) Anti seh, by Santa Cruz Biotechnology (1 mentions) Anti β actin, by Thermo Fisher Scientific (1 mentions) Anti calnexin, by Abcam (1 mentions)

2 protocols using anti meh

Western Blot Analysis of mEH Protein

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A total of 30 μg protein was subjected to 12% SDS-PAGE, and then electrophoretically transferred to a polyvinylidene fluoride (PVDF) membrane (Millipore, Billerica, MA, USA). Protein blots were incubated separately with a panel of specific antibodies such as anti-mEH (1:500 dilution, Santa Cruz Biotechnology) and anti-β-actin (1:4000 dilution, Sigma). An alkaline phosphatase (AP)-conjugated goat anti-mouse IgG was used as a secondary antibody. CDP-Star reagent (Roche Diagnostics) was used for color development.

Chen J.Y., Chen W.N., Jiao B.Y., Lin W.S., Wu Y.L., Liu L.L, & Lin X. (2014). Hepatitis B spliced protein (HBSP) promotes the carcinogenic effects of benzo [alpha] pyrene by interacting with microsomal epoxide hydrolase and enhancing its hydrolysis activity. BMC Cancer, 14, 282.

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Quantifying Epoxide Hydrolase Proteins

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Levels of mEH protein in microsomes and sEH protein in S9 fractions were determined by immunoblot analysis as previously described (Li et al., 2022) , using the following primary antibodies: anti-calnexin (1:1200, Abcam, Waltham, MA, USA, ab22595); anti-β-actin (1:1000, Invitrogen, MA1-140); anti-mEH (1:100, Santa Cruz Biotechnology, SC-135984); anti-sEH (1:100; Santa Cruz Biotechnology; SC-166961). Calnexin and β-actin served as loading controls for microsomes and S9 fractions, respectively. Horseradish peroxidase-conjugated anti-rabbit IgG (1:3000; Sigma-Aldrich, A4914) or anti-mouse IgGκ (1:4000; Santa Cruz Biotechnology; SC-516102) were used as secondary antibodies. For quantitative analysis, relative abundances of target proteins among samples were compared after normalization by the loading control.

Li H., Fan X., Ding X, & Zhang Q.Y. (2024). Tissue-, Region-, and Gene-Specific Induction of Microsomal Epoxide Hydrolase Expression and Activity in the Mouse Intestine by Arsenic in Drinking Water. Drug metabolism and disposition: the biological fate of chemicals, 52(7).

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