Mouse anti gfp

The following primary antibodies were used: mouse anti-ß-Actin and mouse anti-FLAG (Sigma-Aldrich, St. Louis, MO, USA); mouse anti-Alix, mouse anti-EAP20, rabbit anti-EAP30, rabbit anti-EAP45, and mouse anti-Tsg101 (Santa Cruz Biotechnology, Dallas, TX, USA); mouse anti-GFP (BD Biosciences, Heidelberg, Germany); mouse anti-HA (Covance, Princeton, NJ, USA); and rabbit anti-Nedd4 (Cell Signaling, Danvers, MA, USA). Peroxidase-labeled, secondary antibodies were obtained from Dianova (Hamburg, Germany), and fluorophore-labeled antibodies were purchased from Molecular Probes (Eugene, OR, USA).

Human MDA-9/Syntenin cDNA were obtained from H1299 by RT-PCR. Its full length or deletion constructs were sub-cloned into pACT2, pcDNA3.1-HA, pCMV-tag2 (Clontech, CA, USA), pEGFP (Invitrogen), and pLKO.1-AS2-neo vectors for the production of lentivirus or pET28a vectors for bacterial expression by standard molecular cloning procedure. Flag-tagged Slug was as described previously [10 (link)]. Full length or deletion constructs of Slug were sub-cloned into pAS2–1, pCI-neo, pcDNA3.1-HA, pDsRED, and AS2-neo vectors. pcDNA3-HDAC1-Flag was kindly provided by Dr. Wen-Ming Yang (Institute of Molecular Biology, National Chung Hsing University, Taichung, Taiwan).
The primary antibodies used for immunoblot analysis were anti-Syntenin (mouse monoclonal, S-31 or rabbit polyclonal, H-48, Santa Cruz Biotechnology, CA, USA and rabbit monoclonal, Abcam, MA, USA), goat anti-Slug (Santa Cruz Biotechnology), goat anti-HDAC1 (Santa Cruz Biotechnology) and mouse anti-β-actin (Santa Cruz Biotechnology), mouse anti-Flag (Sigma-Aldrich, MO, USA), mouse anti-HA (Covance, Berkeley, California, USA), mouse anti-E-cadherin (BD Biosciences, Inc., CA, USA), mouse anti-vimentin (BD Biosciences), mouse anti-N-cadherin (BD Biosciences), and mouse anti-GFP (BD Biosciences).