All reagents were purchased and used as received from Sigma-Aldrich (St. Louis, MO) unless otherwise noted. Murabutide, ovalbumin (OVA, Ovalbumin EndoFit), OVA257–264, and synthetic monophosphoryl lipid A (MPL) were purchased from InvivoGen (San Diego, CA). Vaccine adjuvant Alhydrogel (Invivogen) was a generous gift given by Dr. Jenny Ting (Department of Microbiology and Immunology, University of North Carolina at Chapel Hill). Triethylamine (TEA) (0.01% v/v) was added to purified water (Millipore Milli-Q Integral Water Purification System, Billerica, MA) to achieve a pH level of 9.0 (basic water) in presence of acetal-containing materials.
Synthetic monophosphoryl lipid a
Manufactured by InvivoGen
Synthetic monophosphoryl lipid A is a purified, detoxified derivative of the lipopolysaccharide (LPS) lipid A component. It functions as a potent immunostimulant, capable of activating the innate immune system.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using synthetic monophosphoryl lipid a
1
Murabutide-Adjuvanted OVA Vaccine Preparation
2
Induction of Alloantigen-Specific Tr1 Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
CD14+ cells were isolated from PBMCs using the manufacturer’s protocol for CD14+ microbeads (Miltenyi) and differentiated into mature DC (matDC) or DC-10 as previously described (32 (link)) with the following minor changes: CD14+ cells were cultured in a base media using 10% human AB serum and matDC were differentiated with 5µg/mL of synthetic monophosphoryl lipid A (InvivoGen) on day 5. Cells were collected on day 7 and irradiated at 6,000 rads. Naïve CD4+ T cells were isolated from a donor allogenic to the CD14+ cell donor used and then co-cultured at a 10:1 ratio (T cell:DC) for 10 days. The control allo-antigen specific T cells cultured with matDC (“T-allo cells”) were cultured without cytokine supplements and the T cells culture with DC-10 that ultimately become the in vitro induced allogenic-antigen specific Tr1 cells (“T-allo10 cells”) were cultured with 10ng/mL rhIL-10 added on day 0 and day 5 (32 (link), 33 (link)). Cells were collected after 10 days of co-culture.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!