Gcms tq8050 nx

The plant material was harvested manually in the rainy season from crops without the use of agrochemicals. The plants grew in moderately acidic soil with 2% organic matter in the indigenous community of Atanquez, Colombia, and were located 1200 m above sea level (10°42′15″ N, 73°17′18″ W). This community is home to the ASOPROKAN association, which is made up of agroecological producers from the indigenous community of the Kankuamos. The essential oil of C. citratus was extracted using steam distillation [16 (link)]. The EO was analyzed in the natural products laboratory of the Universidad de Córdoba, located in Montería, Colombia. A phytochemical evaluation was performed using gas chromatography with subsequent gas characterization/mass spectrometry (GCMS-TQ8050 NX Shimadzu, Kyoto, Japan) using a selective detector. Presumptive identification was performed using Apolar DB-5MS (60 m) and Polar DB-WAX (60 m) columns and compared with information from the NIST (National Institute of Standards and Technology) in Gaithersburg, MD, USA, and the MassLab program (Porto, Portugal).

The amino acid compositions of SPWL were determined using the Shimadzu-GCMS-TQ8050 NX (Kyoto, Japan). The amino acid content was given as g/100 g dry sample. Furthermore, the essential amino acid index (EAAI) and biological value (BV) were estimated using the formula given below by Chinarak et al. [4 (link)]: $$\mathrm{EAAI}=\sqrt[9]{\frac{\mathrm{g} \mathrm{of} \mathrm{lysine} \mathrm{in} 100 \mathrm{g} \mathrm{of} \mathrm{analysis} \mathrm{protein} \times 100}{\mathrm{g} \mathrm{of} \mathrm{lysine} \mathrm{in} 100 \mathrm{g} \mathrm{of} \mathrm{reference} \mathrm{protein}}\times \left(\mathrm{etc}. \mathrm{for} \mathrm{other} 8 \mathrm{EAA}\right)}$$
$$\mathrm{BV}=1.09\times \mathrm{EAAI}-11.7$$

All analyses were performed on a Shimadzu GCMS-TQ8050 NX, consisting of a GC2030 coupled to a triple-quadrupole mass spectrometer detector (TQ-MS) (Shimadzu, Germany), equipped with an AOC20i autosampler. The chromatographic column was a 30 m × 0.25 mm i.d. × 0.5 μm d_f SLB-5ms capillary column [(silphenylene polymer, practically equivalent in polarity to poly (5% dipheny l/95% methylsiloxane)] kindly obtained from MilliporeSigma (Belfonte, PA, USA). GC oven temperature program: 80 °C to 160 °C at 20 °C/min and to 340 °C at 5 °C/min hold 1 min. Carrier gas: helium in constant linear velocity mode at 35.9 cm/s, corresponding to an initial inlet over-pressure of 45.6 kPa. The MS was operated in single-quadrupole mode, in EI mode at 70 eV. The ion source and transfer line temperatures were 200 °C and 280 °C, respectively. The scan range was set to 50–700 m/z, with an acquisition frequency of 10 Hz. Data were acquired using Shimadzu GCMSolution ver 4.45 (Shimadzu, Germany). NIST17s MS commercial libraries were used for putative identification.

A GC-MS-TQ8050 NX (Shimadzu Corporation, Kyoto, Japan) was used to analyze the EO of the three cultivars at the Central Instrumentation Laboratory of the Central University of Punjab in Bathinda, India. With a split ratio of 5, the split injector temperature was set at 280 °C. The oven’s temperature was programmed from 0 to 40 °C and then increased to 220 °C with a hold period of three minutes and five minutes, respectively. Thereafter, the temperature was further increased to 250 °C and held for 5 min. Helium was used as the carrier gas, flowing at a constant rate of 1 mL min⁻¹, with the ion source temperature set at 230 °C, interface temperature of 250 °C and a mass scan range of 40–800 amu. The column flow was set at 1.00 mL min⁻¹, while the column oven temperature was set at 40 °C. The volume used for sample loading and injection was 1 µL. Using the NIST17R library and NIST17M2 library, the various bioactive compounds of the EO were analyzed based on m/z ratio and their retention time. The menthol content in the control and treated plants was estimated by calculating the peak area of the chromatogram.

The amino acid composition of SPWL was analyzed according to Chinarak et al. [15 (link)] using a Shimadzu GCMS-TQ8050 NX (Kyoto, Japan). The concentration of amino acids in each dry sample was expressed in milligrams per gram. The essential amino acid index (EAAI) and biological value (BV) were calculated using the formulae proposed by Kulma et al. [18 (link)]: $$\mathrm{E}\mathrm{A}\mathrm{A}\mathrm{I}=\sqrt[9]{\frac{\mathrm{g} \mathrm{o}\mathrm{f} \mathrm{l}\mathrm{y}\mathrm{s}\mathrm{i}\mathrm{n}\mathrm{e} \mathrm{i}\mathrm{n} 100 \mathrm{g} \mathrm{o}\mathrm{f} \mathrm{a}\mathrm{n}\mathrm{a}\mathrm{l}\mathrm{y}\mathrm{s}\mathrm{i}\mathrm{s} \mathrm{p}\mathrm{r}\mathrm{o}\mathrm{t}\mathrm{e}\mathrm{i}\mathrm{n}\times 100}{\mathrm{g} \mathrm{o}\mathrm{f} \mathrm{l}\mathrm{y}\mathrm{s}\mathrm{i}\mathrm{n}\mathrm{e} \mathrm{i}\mathrm{n} 100 \mathrm{g} \mathrm{o}\mathrm{f} \mathrm{r}\mathrm{e}\mathrm{f}\mathrm{e}\mathrm{r}\mathrm{e}\mathrm{n}\mathrm{c}\mathrm{e} \mathrm{p}\mathrm{r}\mathrm{o}\mathrm{t}\mathrm{e}\mathrm{i}\mathrm{n}}\times (\mathrm{e}\mathrm{t}\mathrm{c}. \mathrm{f}\mathrm{o}\mathrm{r} \mathrm{o}\mathrm{t}\mathrm{h}\mathrm{e}\mathrm{r} 8 \mathrm{E}\mathrm{A}\mathrm{A})}$$
$$\mathrm{B}\mathrm{V}=1.09\times \mathrm{E}\mathrm{A}\mathrm{A}\mathrm{I}-11.7$$