Easysep human cd11b positive selection kit

Manufactured by STEMCELL

Sourced in Canada

The EasySepTM Human CD11b Positive Selection Kit is a laboratory tool designed for the isolation and enrichment of CD11b-positive cells from human samples. The kit utilizes magnetic particles and an easy-to-use procedure to selectively separate the target cell population.

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Lab products found in correlation

Interleukin 4 (il 4), by Gentaur (2 mentions) Granulocyte macrophage colony stimulating factor, by Gentaur (2 mentions)

Close spelling variants of this product

EasySep CD11b positive selection kit EasySep kits

2 protocols using easysep human cd11b positive selection kit

Isolation and Differentiation of Dendritic Cells

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Peripheral blood mononuclear cells were isolated from blood of healthy donors by means of Ficoll density gradient centrifugation. Next, the EasySepTM Human CD11b positive selection Kit (STEMCELL Technologies Inc) was used to isolate macrophages. The harvested cells were then incubated in a medium supplemented with granulocyte macrophage colony-stimulating factor (500 IU/ml, Gentaur) and IL-4 (248 IU/ml, Gentaur). After 5–6 days, a population of immature DCs were obtained for cocultures with ESCC cells.

Luo H., Wang X., Song S., Wang Y., Dan Q, & Ge H. (2022). Targeting stearoyl-coa desaturase enhances radiation induced ferroptosis and immunogenic cell death in esophageal squamous cell carcinoma. Oncoimmunology, 11(1), 2101769.

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Isolation and Differentiation of Dendritic Cells

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Freshly harvested blood samples were collected from healthy donors, and peripheral blood mononuclear cells (PBMCs) were isolated by means of Ficoll density gradient centrifugation. Macrophages were purified using the EasySep^TM Human CD11b positive selection Kit (STEMCELL Technologies Inc, Canada). Cells were then incubated in a medium supplemented with IL-4 (248 IU/ml, Gentaur, UK) and granulocyte macrophage colony-stimulating factor (500 IU/ml, Gentaur), and cultured for 5–6 days to obtain a population of immature DCs.

Luo H., Sun Y., Wang L., Liu H., Zhao R., Song M, & Ge H. (2023). Targeting endoplasmic reticulum associated degradation pathway combined with radiotherapy enhances the immunogenicity of esophageal cancer cells. Cancer Biology & Therapy, 24(1), 2166763.

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