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5 protocols using acetaldehyde

1

Quantitative Analysis of Food Volatiles

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All chemicals used had a purity grade higher than 95%. Hexanal, benzaldehyde, 2-methylpropanal, nonanal, 2-methylbutanal, 3-methylbutanal, and 4-fluorobenzaldehyde standards were purchased from Sigma-Aldrich (Steinheim, Germany). Acetaldehyde, phenylAcetaldehyde, 5-(hydroxymethyl) furfural, 2-furaldehyde, and trans-2-nonenal were purchased from Acros Organics (Geel, Belgium). Absolute ethanol, acetonitrile, and methanol (HPLC grade 99.99%) was obtained from Sigma-Aldrich (Steinheim, Germany). Sodium chloride was obtained from Panreac (Barcelona, Spain). Ultra-pure water with a resistivity of >18 MΩ.cm (type 1) was obtained from a Millipore Simplicity® UV apparatus (Milford, MA, USA). The alkane solution (C7–C30) was obtained from Supelco (Sigma Aldrich, St. Louis, MO, USA).
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2

Volatile Compound Analysis by GC-MS

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All chemicals used had a purity grade higher than 95%. Hexanal, benzaldehyde, 2-methylpropanal, nonanal, 2-methylbutanal, 3-methylbutanal, and 4-fluorobenzaldehyde standards were purchased from Sigma-Aldrich (Steinheim, Germany). Acetaldehyde, phenylAcetaldehyde, 5-(hydroxymethyl) furfural, 2-furaldehyde and trans-2-nonenal were purchased from Acros Organics (Geel, Belgium). Absolute ethanol, acetonitrile, and methanol (all HPLC grade 99.99%) came from Sigma-Aldrich (Steinheim, Germany). Sodium chloride was obtained from Panreac (Barcelona, Spain). Ultra-pure water with a resistivity of >18 MΩ·cm (type 1) was obtained from a Millipore Simplicity® UV apparatus (Milford, MA, USA). The alkane solution (C7–C30) was obtained from Supelco (Sigma Aldrich, St. Louis, MO, USA).
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3

Enzyme Immobilization on Silica Beads

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Acetaldehyde, 1,4-benzoquinone, Pluronic P123, 3-aminopropyltriethoxysilane (APTES) and (3-methylaminopropyl) trimethoxysilane (APTMS) were acquired from Acros Organics (Waltham, MA, USA). Potassium dihydrogen phosphate, dipotassium hydrogen phosphate, hydrochloric acid (HCl), and tetraethyl ortosilicate (TEOS) were obtained from Lach-Ner (Neratovice, Czech Republic). o-Benzylhydroxylamine hydrochloride was acquired from TCI (Oxford, UK). Bovine serum albumin (BSA), succinic anhydride, glutaraldehyde, and chloroAcetaldehyde solution (50% (w/w)) were bought from Sigma-Aldrich (Darmstadt, Germany). Ethanol absolute was obtained from Scharlau (Barcelona, Spain). DERA024 from Thermotoga maritima was purchased from Prozomix (Haltwhistle, UK). Acetonitrile, trifluoracetic acid (TFA), and 1,2,4-Trimethylbenzene were acquired from Fisher Scientific (Loughborough, UK).
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4

Inflammasome Activation Pathway Protocol

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LPS, sodium orthovanadate, SEITU and monoclonal anti-mouse β-actin antibodies were from Sigma-Aldrich (St. Louis, MO). Anti-mouse ASC and caspase-1 antibodies were from Santa Cruz Biotechnology Inc. (Dallas, TX), and anti-mouse and human IL-1β antibodies were from R&D Systems (Minneapolis, MN). Biotin conjugated anti- mouse and rabbit secondary antibodies were from GE Healthcare UK Limited (Little Chalfont, Buckinghamshire, UK) and anti-goat IgG was from Jackson ImmunoResearch (West Grove, PA). For inflammasome stimulation, nigericin and poly (dA: dT) were from Invivogen (San Diego, CA), ATP and anthrax lethal factor and protective antigen were from Amersham Biosciences (Piscatawy, NJ) and BEI Resources (Manassas, VA), respectively. Ethanol and carboxy-PTIO were from Pharmco AAPER (Brookfield, CT) and Life Technologies Corporation (Carlsbad, CA). NAD+ was from Cayman Chamical (Ann Arbor, Michigan), and acetaldehyde and 4-methylpyrazole (4-MP) were from Acros Organics (Geel, Belgium). Dr. Michael P. Murphy (Medical Research Council Mitochondrial Biology Unit, University of Cambridge, Cambridge, UK) kindly provided MitoQ.
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5

Synthesis of Crosslinked Hydrogels

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Chemicals used in this work were acquired from different suppliers and used as received. Guar gum (product reference: G4129-250G), diallyl carbonate (CAR), 2,2′-(ethylenedioxy)diethanethiol (EDDET), trimethylolpropane tris(3-mercaptopropionate) (TriT), and methoxypolyethyleneglycol-350 (MeO-PEG-350) were acquired from Sigma-Aldrich (Madrid, Spain). Calcium chloride (CaCl2) was provided by Fisher Scientific (part of Thermo Fisher Scientific; Waltham, MA, USA). Alfa Aesar (part of Thermo Fisher Scientific; MA, USA) was the supplier of (2R,3R)-N,N′-diallyltartramide (TAR) and allyl alcohol. 2,2-Dimethoxy-2-phenylacetophenone (DMPA) and acetaldehyde were purchased from Acros Organics (part of Thermo Fisher Scientific; MA, USA).
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