Anti erk sc 514302

Manufactured by Santa Cruz Biotechnology

Sourced in United States

Anti-ERK (sc-514302) is an antibody product offered by Santa Cruz Biotechnology. It is designed to detect extracellular signal-regulated kinase (ERK) proteins. The antibody can be used in various laboratory applications to identify and study ERK proteins.

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Lab products found in correlation

Anti cleaved caspase 3, by Cell Signaling Technology (1 mentions) Anti p mek ser217 221, by Cell Signaling Technology (1 mentions) Ab18251, by Abcam (1 mentions) Ab228461, by Abcam (1 mentions) Ab124794, by Abcam (1 mentions) Anti ph3 ser10, by Merck Group (1 mentions) Anti ki67, by Cell Signaling Technology (1 mentions) Anti mek, by Cell Signaling Technology (1 mentions) Ab8245, by Abcam (1 mentions) Anti tubulin, by Merck Group (1 mentions) Anti akt sc 5298, by Santa Cruz Biotechnology (1 mentions) Anti parp, by BD (1 mentions) Ripa lysis buffer, by Beyotime (1 mentions) Enhanced bca protein assay kit, by Beyotime (1 mentions) Anti gapdh 5174, by Cell Signaling Technology (1 mentions) Hrp labeled goat anti mouse igg h l antibody, by Beyotime (1 mentions)

Spelling variants of this product

Anti-ERK (116 citations) Sc-514302 (19 citations) ERK (sc-514302) (4 citations)

4 protocols using anti erk sc 514302

Antibody Characterization for BRAF Study

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The commercial primary antibodies in this paper were as follows: anti-REPO (8D12, DSHB); anti-pH3 (Ser10) (H0412, Millipore); anti-Cleaved-Caspase 3 (9664, Cell Signaling); anti-α tubulin (ab18251, abcam); anti-MEK (8727, Cell Signaling); anti-p-MEK (Ser217/221) (3958, Cell Signaling); anti-ERK (sc-514302, Santa Cruz); anti-p-ERK (T202/Y264) (4370, Cell Signaling); anti-KCNMA1 (sc-374142, Santa Cruz); anti-Ki-67 (9129, Cell Signaling); and anti-GAPDH (ab8245, abcam).
Three BRAF-related antibodies were used in this study: anti-BRAF (sc-5284, Santa Cruz) were used to detect the total levels of BRAF; anti-BRAF^V600E specifically recognizing mutated amino acid sequence (ab228461, abcam) were used to detect the levels of BRAF^V600E; anti-p-BRAF (S729) specifically recognizing phosphorylated site (ab124794, abcam) were used to detect the levels of BRAF with serine 729 phosphorylation, to measure the BRAF activation levels regardless of the presence of BRAF mutations.

Xie S., Xu C., Wu C., Lou Y., Duan J., Sang R., Lou Z., Hou J., Ge W., Xi Y, & Yang X. (2023). Co-dependent regulation of p-BRAF and potassium channel KCNMA1 levels drives glioma progression. Cellular and Molecular Life Sciences, 80(3), 61.

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Comprehensive EGFR and Signaling Pathway Antibodies Protocol

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Antibodies against EGFR (#4267), IGF-1R (#9750), IR (#3025), VEGF2 (#2479), p-AKT (#4060), p-ERK (#4370), AKT (#9272), ERK (#4695), TFEB (#4240), FOX3a (#2497 and #12829), EPCAM (#2929), actin (#4970) and LC3B (#3868 and #2775) were purchased from Cell Signaling Technology (Danvers, MA). Anti-PARP was purchased from BD Biosciences (San Jose, CA). Anti-tubulin was purchased from Sigma (St. Louis, MO). Anti-AKT (sc-5298) and anti-ERK (sc-514302) were purchased from Santa Cruz (Dallas, TX). Anti-VEGFR1 (32152) was purchased from Abcam (Cambridge, MA). CXCR1 (55450–1-AP) was purchased from Proteintech (Rosemont, IL). CXCR2 (PA1–31217) was purchased from Fisher (Waltham, MA). An anti-DIRAS3 murine monoclonal antibody (ID8) was generated in our laboratory. Doxycycline hyclate (DOX, D9891), Vascular Endothelial Growth factor (VEGF, V-7259), Interleukin-8 human (IL8, I1645), Insulin-Like Growth Factor-I human (IGF-1, I3769) and AKT 1/2 kinase inhibitor (A6730) were purchased from Sigma-Aldrich (St. Louis, MO). For MAPK inhibitors, U0126 (#9903), SB203500 (#5633) were purchased from Cell Signaling Technology and JNK inhibitor II was purchased from Calbiochem (Burlington, MA)

Mao W., Peters H.L., Sutton M.N., Orozco A.F., Pang L., Yang H., Lu Z, & Bast RC J.r. (2019). The Role of VEGF, IL-8 and IGF in Sustaining Autophagic DIRAS3-induced Dormant Ovarian Cancer Xenografts. Cancer, 125(8), 1267-1280.

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Antibody Sources and siRNA Targets

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Antibodies against LC3 (2775S), β-actin (4910L), pAkt^S473 (9271S), AKT (9272), mTOR (2972), p-mTOR^S2448 (2971), E2F1 (3742), p-P70S6K^T389 (9205), P70S6K (2708), p-ULK1^S757 (6888), ULK1 (4773) p-ERK^T202/Y204 (4370), ERK (4695) were purchased from Cell Signaling Technology (Danvers, MA, USA). Antibodies against DIRAS3 (ARHI) were generated in our laboratory, and do not show cross reactivity with other members of the DIRAS family. P62/SQSTM1 antibody was purchased from MBL (Woburn, MA, USA). Anti-E2F4 (10923-1-AP) antibody was purchased from proteintech (Rosemont, IL, USA). Anti-CEBPα D-5 (sc-365318), anti-AKT (sc-5298) and anti-ERK (sc-514302) antibodies was purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA).
siRNAs were purchased from Dharmacon (Lafayette, CO, USA) and used as suggested by the manufacturer. E2F1 (1869) E2F4 (1874) DIRAS3 (9077) Sestrin2 (83667) CEBPα (1050) Non-targeting control (1810).

Sutton M.N., Huang G.Y., Zhou J., Mao W., Langley R., Lu Z, & Bast RC J.r. (2019). Amino Acid Deprivation-Induced Autophagy Requires Upregulation of DIRAS3 through Reduction of E2F1 and E2F4 Transcriptional Repression. Cancers, 11(5), 603.

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Molecular Mechanisms of XJR-Induced Apoptosis

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After treatment with XJR for 24 h, A549 cells were harvested and lysed on ice in RIPA lysis buffer (Beyotime, Shanghai, China). Protein concentration was detected by using an Enhanced BCA Protein Assay Kit (Beyotime, Shanghai, China). We isolated 40 μg total protein samples by SDS-polyacrylamide gels (PAGE) and electroblotted them onto PVDF-membranes. All membranes were then blocked with 5% defatted milk and then incubated with primary antibodies. Subsequently, membranes were washed with TBST 3 times and incubated with HRP-labeled Goat Anti-Mouse IgG (H+L) antibody (A0216, Beyotime, Shanghai, China). All blots were then developed with an enhanced chemiluminescence (ECL) reagent and analyzed by ImageJ software, which was normalized by GAPDH. Anti-Bcl-2 (4223T), anti-Bax (5023T), anti-cleaved caspase-3 (9661T), anti-cleaved caspase-9 (9509T), anti-MMP2 (40994S), anti-MMP9 (13667T), anti-p38 (8690T), anti-JNK (9258T), anti-p-p38 (4511T), anti-p-ERK (4370T), anti-p-JNK (4668T), and anti-GAPDH (5174S) antibodies were from Cell Signaling Technology (Boston, MA, USA). Anti-ERK (sc-514302) was obtained from Santa Cruz Biotechnology (CA, USA).

Wang Y., Xu C., Xu B., Li L., Li W., Wang W, & Wu M. (2019). Xiaoai Jiedu Recipe Inhibits Proliferation and Metastasis of Non-Small Cell Lung Cancer Cells by Blocking the P38 Mitogen-Activated Protein Kinase (MAPK) Pathway. Medical Science Monitor : International Medical Journal of Experimental and Clinical Research, 25, 7538-7546.

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