Alexa fluor 488 34106es60

Zika virus envelope (E) protein antibody (GTX133314) was purchased from GeneTex (San Antonio, USA). Dengue virus type 1–4 antibody (MA1-27093) was purchased from Invitrogen (Shanghai, China). Abs against Flag (F1804) and Myc (A00863-100) were purchased from Sigma–Aldrich (Shanghai, China) and GenScript (Nanjing, China), respectively. Abs against HA (66006-2-lg) and GAPDH (60004-1-lg) were purchased from ProteinTech Group (Wuhan, China). Anti-sodium potassium ATPase (Na⁺/K⁺-ATPase) antibody (EP1845Y) was kindly offered by Jing Yao from the College of Life Sciences at Wuhan University. Alexa Fluor 488 (34106ES60) and Alexa Fluor 647 AffiniPure Donkey Anti-Mouse (34113ES60) were purchased from YEASEN (Shanghai, China). LysoSensor™ Green DND-189 (L7535) and Thermo Fisher Scientific Page Ruler Prestained Protein Ladder (26617) were purchased from Invitrogen (Shanghai, China). Bestar^® SYBR Green qPCR master mix reagent (DBI-2073) was purchased from DBI^® Bioscience (Shanghai, China).

Antibodies against P-p38 MAPK (4511), p38 MAPK (9212), and P-MKK3/6 (12280) were purchased from Cell Signaling Technology (Beverly, MA). Antibodies against the HCV core protein (C7-50) (sc-57800) and TAB1 (B-3) (sc-166138) were purchased from Santa Cruz Biotechnology (Dallas, Texas, USA). Antibodies against HA (66006-2-lg and 51064-2-AP), Flag (20543-1-AP), TAB1 (27566-1-AP) and GAPDH (60004-1-lg) were obtained from ProteinTech Group (Wuhan, China). Alexa Fluor 488 (34106ES60), and Cy3 (33108ES60) were purchased from YEASEN (Shanghai, China). SB203580 (S1076) was purchased from Selleck Chemicals (Houston, TX). Dynabeads® Protein G (10004D) was from Invitrogen (Waltham, Massachusetts).