Lyz2 lyzm cre mice

All experiments were performed according to NIH guidelines for the care and use of animals in research and under protocol approved by IACUC at the University of Maryland, Baltimore. All experiments were performed on young adult male mice (9–12 weeks old, 21–27 g). Sham and CCI surgeries were performed on the following mice: Cx3cr1-GFP mice (The Jackson Laboratory, 005582), Ccr2-RFP mice (The Jackson Laboratory, 017586), C57Bl/6 mice (The Jackson Laboratory, 00064), transgenic C57Bl/6 mice expressing GFP-LC3 [64 (link)], ROSA^tdTomato mice (The Jackson Laboratory, 007914), Lyz2/LyzM-Cre mice (The Jackson Laboratory, 004781) and Becn1/Beclin^flox/flox mice (The Jackson Laboratory, 028794). Lyz2/LyzM-Cre mice and Becn1/Beclin^flox/flox mice were crossed with each other for two generations to produce Becn1^flox/flox; Lyz2^Cre/Cre knockout mice, all mice genotyped for flox and Cre alleles according to the The Jackson Laboratory genotyping protocol. All mice were housed on sterilized bedding in a specific pathogen- free facility (12 h light/dark cycle).