Lc3 rabbit polyclonal antibody

Manufactured by Merck Group

Sourced in Italy

The LC3 rabbit polyclonal antibody is a laboratory reagent used for the detection and analysis of the LC3 protein in various biological samples. LC3 is a widely used marker for the process of autophagy, a cellular mechanism involved in the degradation and recycling of cellular components. The antibody can be utilized in techniques such as Western blotting, immunohistochemistry, and immunofluorescence to study the expression and localization of the LC3 protein in research applications.

Automatically generated - may contain errors

Lab products found in correlation

2 protocols using lc3 rabbit polyclonal antibody

Comprehensive Molecular Profiling of Cell Death

Check if the same lab product or an alternative is used in the 5 most similar protocols

3-Bromopyruvate (3BP), ATP Bioluminescent Assay Kit, 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT), 2′,7′-dichlorodihydrofluorescein diacetate (H₂DCFDA), MitoTEMPO, antimycin A, menadione, perifosine, digitonin, staurosporine, erythrosin B, LC3 rabbit polyclonal antibody, and actin mouse monoclonal antibody were from Sigma Aldrich. Cyt c mouse monoclonal, COX-IV mouse monoclonal, p53 mouse monoclonal, goat anti-mouse HRP-conjugated IgG, goat anti-rabbit HRP-conjugated IgG, and rabbit anti-goat HRP-conjugated IgG antibodies were from Santa Cruz Biotechnology. Akt rabbit polyclonal, p-Akt (Ser-473) rabbit monoclonal, JNK rabbit monoclonal, p-JNK (Thr183/Tyr185) rabbit monoclonal, p44/42 MAPK (ERK1/2) rabbit monoclonal, p-ERK1/2 (Thr202/Tyr204) rabbit monoclonal, p38 MAPK rabbit polyclonal, p-p38 (Thr180/Tyr182) rabbit monoclonal, p-p53 (Ser-15) rabbit polyclonal, caspase-3 rabbit polyclonal, and caspase-9 rabbit polyclonal antibodies were from Cell Signaling Technology. Beta tubulin mouse monoclonal antibody was from Proteintech. 5,5′,6,6′-tetrachloro-1,1′,3,3′tetraethylbenzimidazolylcarbocyanine iodide (JC-1) and MitoSOX™ were from Molecular Probes.

Petricciuolo M., Davidescu M., Fettucciari K., Gatticchi L., Brancorsini S., Roberti R., Corazzi L, & Macchioni L. (2020). The efficacy of the anticancer 3-bromopyruvate is potentiated by antimycin and menadione by unbalancing mitochondrial ROS production and disposal in U118 glioblastoma cells. Heliyon, 6(12), e05741.

+ Open protocol

+ Expand

Mitochondrial Modulation and Autophagy Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

Bromopyruvate (3BP), lonidamine (LND), mitochondrial inhibitors, respiratory substrates, chemicals, LC3 rabbit polyclonal antibody, α-tubulin and β-tubulin mouse monoclonal antibodies, and goat anti-mouse FITC-conjugated IgG were from Sigma (Milan, Italy). Cyt c mouse monoclonal antibody, AIF goat polyclonal antibody, β-actin goat polyclonal antibody, p53 (FL-393) rabbit polyclonal antibody, p-p53 (Ser-315) rabbit polyclonal antibody, HK-II (C-14) goat polyclonal antibody, beclin 1 (E-8) mouse monoclonal antibody, goat anti-mouse HRP-conjugated IgG, goat anti-rabbit HRP-conjugated IgG, and rabbit anti-goat HRP-conjugated IgG were from Santa Cruz Biotechnology; VDAC1 rabbit polyclonal antibody was from Calbiochem. p-p53 (Ser-15) rabbit polyclonal, SQSTM1/p62 rabbit polyclonal, Akt rabbit polyclonal, and p-Akt (Ser-473) rabbit monoclonal antibodies were from Cell Signaling Technology. LC3 mouse monoclonal antibody was from NanoTools. Mito-tracker red CM-XRos, Alexa Fluor® 488 goat anti-rabbit IgG, and Alexa Fluor® 555 goat anti-mouse IgG were Molecular Probes products.

Davidescu M., Macchioni L., Scaramozzino G., Cristina Marchetti M., Migliorati G., Vitale R., Corcelli A., Roberti R., Castigli E, & Corazzi L. (2015). The energy blockers bromopyruvate and lonidamine lead GL15 glioblastoma cells to death by different p53-dependent routes. Scientific Reports, 5, 14343.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!