A2780

Manufactured by Corning

Sourced in United States

The A2780 is a laboratory equipment product manufactured by Corning. It is designed for use in scientific research and analysis. The core function of the A2780 is to provide a controlled environment for various experimental and testing procedures. No further details or interpretation of the intended use are provided.

Automatically generated - may contain errors

Lab products found in correlation

Rpmi 1640 medium, by Corning (7 mentions) Caov3, by Corning (6 mentions) Skov3, by American Type Culture Collection (5 mentions) A2780, by American Type Culture Collection (5 mentions) Caov3, by American Type Culture Collection (5 mentions) Skov3, by Corning (5 mentions) Ovcar 3, by Corning (5 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (5 mentions) Medium 199, by Thermo Fisher Scientific (4 mentions) Rpmi 1640, by Corning (4 mentions) Mcdb 105, by Merck Group (3 mentions) Ovcar3, by American Type Culture Collection (3 mentions) Dulbecco modified eagle medium (dmem), by Corning (3 mentions) A2780, by European Collection of Cell Cultures (3 mentions) Bright line hemacytometer, by Merck Group (3 mentions) Ovcar5, by American Type Culture Collection (3 mentions) Mycoalert mycoplasma detection kit, by Lonza (3 mentions) Ovcar5, by Corning (3 mentions) Igrov1, by American Type Culture Collection (2 mentions) Hank s balanced salt solution, by STEMCELL (2 mentions)

17 protocols using a2780

Isolation and Culture of Ovarian Cancer Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

The human OC cell lines SKOV3, IGROV1 and A2780 were from the American
Type Culture Collection (Rockville, MD, USA). De-identified OC ascites samples
were obtained through an IRB approved protocol of the Indiana University Simon
Cancer Center Tissue Bank. Ascites tumor cells were collected by centrifugation
at 200 × g for 3 min. Erythrocytes were lysed by re-suspending the cell
pellet in a 1:4 mixture of cold Hank's balanced salt solution modified (StemCell
Technologies) supplemented with 2% FBS and red blood cell lysis buffer (0.8%
ammonium chloride, 0.1 mM EDTA, pH 7.4) for 5 min. After centrifugation at 350
× g for 5 min, 25,000 ascites derived tumor cells were cultured as
monolayers or spheroids. SKOV3 and primary OC cells were cultured in media
containing 1:1 MCDB 105 (Sigma) and M199 (Cellgro, Herndon, VA, USA)
supplemented with 10% FBS and antibiotics, while IGROV1 and A2780 cells were
grown in RPMI 1640 at 37°, under a humidified atmosphere containing 5%
CO2.

Condello S., Morgan C.A., Nagdas S., Cao L., Turek J., Hurley T.D, & Matei D. (2014). Beta-Catenin Regulated ALDH1A1 is a Target in Ovarian Cancer Spheroids. Oncogene, 34(18), 2297-2308.

+ Open protocol

+ Expand

Isolation and Culture of Ovarian Cancer Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Condello S., Morgan C.A., Nagdas S., Cao L., Turek J., Hurley T.D, & Matei D. (2014). Beta-Catenin Regulated ALDH1A1 is a Target in Ovarian Cancer Spheroids. Oncogene, 34(18), 2297-2308.

+ Open protocol

+ Expand

Culturing Human Ovarian Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols

Human ovarian cancer cell lines SK‐OV‐3 and A2780 were purchased from Shanghai Biological Technology Co., Ltd. enzyme research (Shanghai, China). Human normal ovarian epithelial cell IOSE80 was purchased from Otwo Biotech (Shenzhen, China). A2780 were maintained in RPMI 1640 medium (Corning, China) containing 10% foetal bovine serum (FBS, Biological Industries, Israel); SK‐OV‐3 were maintained in McCOY’s 5A medium (JiNuo Biology, China) and IOSE80 were maintained in DMEM medium (Corning, China) containing 10% fetal bovine serum (FBS, Biological Industries, Israel). Cells were grown in incubator with a humidified atmosphere of 5% CO2 at 37°C.

Dong P., Fu H., Chen L., Zhang S., Zhang X., Li H., Wu D, & Ji X. (2020). PCNP promotes ovarian cancer progression by accelerating β‐catenin nuclear accumulation and triggering EMT transition. Journal of Cellular and Molecular Medicine, 24(14), 8221-8235.

+ Open protocol

+ Expand

Ovarian Cancer Spheroid Formation

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

A2780, a human ovarian cancer cell line, was purchased from the American Type Culture Collection (USA). A2780 cells were cultured in RPMI-1640 medium supplemented with 10% fetal bovine serum (FBS) at 37°C in 5% CO₂. A2780 ovarian cancer spheroids were isolated from A2780 cells as previously described (Choi et al., 2016 (link)). In brief, A2780 cells were seeded at Ultra-Low Attachment 6-well culture plates (Corning, USA) with a density of 2 × 10³ cells/10 cm², followed by culturing in a serum-free Neuro Basal Medium supplemented with B-27 Supplement, 10 ng/ml basic fibroblast growth factor, 20 ng/ml epidermal growth factor, 2.5 μg/ml amphotericin B, 100 U/ml penicillin, and 100 μg/ml streptomycin. To measure the spheroid-forming ability, the cells were seeded in 24-well Corning ultra-low-attachment plates (Corning) at a concentration of 1,000 cells/ml and cultured in spheroid culture media. After the suspension cells were cultured for 20 days, the number of spheres was counted using a microscope.

Kim D.K., Kim Y.N., Kim Y.E., Lee S.Y., Shin M.J., Do E.K., Choi K.U., Kim S.C., Kim K.H., Suh D.S., Song P, & Kim J.H. (2021). TRIB2 Stimulates Cancer Stem-Like Properties through Activating the AKT-GSK3β-β-Catenin Signaling Axis. Molecules and Cells, 44(7), 481-492.

+ Open protocol

+ Expand

Culturing Ovarian Cancer Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols

Human EOC cells SK-OV-3, OVCAR-3, CAOV-3 and ES-2 were purchased from the American Type Culture Collection (ATCC; Manassas, VA, USA) and A2780 was obtained from the European Collection of Authenticated Cell Cultures (ECACC, Salisbury, UK). Human non-tumorous ovarian surface epithelial cells (HOSEpiC) were obtained from Guangzhou Jennio Biotech Co., Ltd. (Guangzhou, China). SK-OV-3 and CAOV-3 cells were cultured in DMEM (Corning Life Sciences, Manassas, VA, USA). A2780, OVCAR-3 and HOSEpiC cells were respectively cultured in RPMI-1640 media (Corning Life Sciences). ES-2 cells were cultured in McCoy's 5A medium (Sigma-Aldrich; Merck KGaA, Darmstadt, Germany). All media were supplemented with 10% fetal bovine serum (FBS; Gibco; Invitrogen; Thermo Fisher Scientific, Inc., Waltham, MA, USA) and were replaced with fresh medium every three days.

Lin Q., Guan W., Ren W., Zhang L., Zhang J, & Xu G. (2018). MALAT1 affects ovarian cancer cell behavior and patient survival. Oncology Reports, 39(6), 2644-2652.

+ Open protocol

+ Expand

Ovarian Cancer Cell Culture Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

The human epithelial ovarian cancer cell line, A2780, was purchased from Sigma. The human ovarian adenocarcinoma cell line, 3AO, was obtained from Women's Hospital, School of Medicine, Zhejiang University, which was tested and authenticated. 3AO and A2780 cells were cultured in Roswell Park Memorial Institute (RPMI)-1640 medium (BI, Kibbutz Beit-Haemek, Israel), supplemented with 10% fetal bovine serum (FBS) (Invitrogen, New York, NY, USA) at 37°C and 5% CO₂. The adherent cells were cultured in serum-free medium (SFM) composed of 10uL/mL B27 additive (Life Technologies, Carlsbad, CA, USA), 10 ng/mL, 1 mg/mL insulin (Sigma-Aldrich, Burlington, MA, USA), basic fibroblast growth factor, 20 ng/mL epidermal growth factor (Pepro-Tech, Rocky Hill, CT, USA), Dulbecco's modified Eagle's medium (DMEM/F12) (BI), to form spheroids after plating 5×10⁴ cells per well in ultra-low attachment 6-well culture plates (Corning, New York, NY, USA). The culture medium will be renewed every two or three days. After plating 400 or 600 cells per well in ultra-low attachment 96-well culture plates (Corning), A2780 or 3AO cells were cultured in SFM at 37°C in 5% CO₂ for 7 days. The culture medium will be renewed every two or three days. The spheroids were cultured in RPMI-1640 medium with 10% FBS after plating in 6 Nunclon Delta plates (Thermo Scientific, Suzhou, China) to re-differentiate.

Yue Y., Xia L., Xu S., Wang C., Wang X., Lu W, & Xie X. (2020). SURF4 maintains stem-like properties via BIRC3 in ovarian cancer cells. Journal of Gynecologic Oncology, 31(4), e46.

+ Open protocol

+ Expand

Culturing Human Ovarian Cancer Cell Lines

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

SKOV3, CAOV3, OVCAR3 and A2780 human ovarian cancer cell lines were purchased from American Type Culture Collection. OV75 primary ovarian cancer cells were provided by Dr Jocelyn Reader and Dr Dana Roque, who were the original suppliers (Division of Gynecologic Oncology, University of Maryland School of Medicine, Baltimore, MD, USA). OV75 cells are primary ovarian cancer cells available for research labs from the University of Maryland. The SKOV3, CAOV3 and A2780 cells were cultured in DMEM (Corning, Inc.) supplemented with 10% FBS (Sigma-Aldrich; Merck KGaA) and 1% penicillin/streptomycin (PS). OVCAR3 cells were cultured in RPMI 1640 medium (Corning, Inc.) with 10% FBS and 1% PS. OV75 cells were cultured in HOSE Media [1:1 mixture of MCDB 105 (Sigma-Aldrich; Merck KGaA) and Medium 199 (Thermo Fisher Scientific, Inc.)], 10% FBS, 1% L-glutamine, 1% non-essential amino acids, 1% PS, sodium bicarbonate at pH 7.4) as described previously (17 (link)). All cell lines were grown in a humidified 37°C incubator with 5% CO₂/95% air, and the medium was replaced twice a week.

Zhang R., Roque D.M., Reader J, & Lin J. (2022). Combined inhibition of IL-6 and IL-8 pathways suppresses ovarian cancer cell viability and migration and tumor growth. International Journal of Oncology, 60(5), 50.

+ Open protocol

+ Expand

Establishment and Characterization of Cancer Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols

All cell lines used were of human origin. A549 (non-small cell lung cancer) and MCF-7 (breast cancer) cell lines were purchased from Cell Resource Center of Shanghai Institute for Biological Sciences (Chinese Academy of Sciences, Shanghai, China). A549T (Taxol-resistant A549 subline) and MCF-7R (Adriamycin-resistant MCF-7 subline) cell lines were from Shanghai Aiyan Biological Technology Co. Ltd (Shanghai, China). HCT-8 (colon carcinoma), A2780 (ovarian cancer), HCT-8T (Taxol-resistant HCT-8 subline) and A2780T (Taxol-resistant A2780 subline) were from Nanjing KeyGEN BioTECH Co. Ltd (Nanjing, China). PC-3 (prostate cancer) was from Typical Culture Preservation Commission Cell Bank, Chinese Academy of Sciences (Shanghai, China).
All cell lines were routinely cultured at 37 °C in humidified atmosphere with 5% CO₂. Unless stated otherwise, the growth medium used for A549, A549T, MCF-7R, HCT-8, HCT-8T, PC-3 and A2780T cells was RPMI-1640 (Corning) containing 10% fetal bovine serum (FBS, Gibco) and 1% penicillin/streptomycin (Corning). MCF-7 and A2780 cells were propagated in Dulbecco’s Modified Eagle Medium (DMEM, Corning) with 10% fetal bovine serum (FBS, Gibco) and 1% penicillin/streptomycin (Corning). For comparison, some experiments replaced FBS with other serums (calf serum, bovine serum, goat serum, horse serum or human serum) and the same concentration.

Wang L., Liu Y., Qi C., Shen L., Wang J., Liu X., Zhang N., Bing T, & Shangguan D. (2018). Oxidative degradation of polyamines by serum supplement causes cytotoxicity on cultured cells. Scientific Reports, 8, 10384.

+ Open protocol

+ Expand

Cultivation of Ovarian Cancer Cell Lines

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Human ovarian cancer cell line OVCAR5 (female) was obtained from American Type Culture Collection. A2780 (female) was acquired from European Collection of Cell Cultures. OVCA433 (female) ovarian serous adenocarcinoma cell line was a generous gift from Dr. Marcin Iwanicki (Stevens Institute of Technology, Department of Chemistry and Chemical Biology, NJ USA). OVCAR5 and A2780 were grown in RPMI-1640 medium (Corning, catalogue No. 10-040-CM). OVCA433 (Iwanicki et al., 2016)^{39 (link)} were cultured in 1:1 mixture of MCDB 105 (Cell Applications, catalogue No. 117–500) medium and Medium 199 (Gibco, catalogue No. 11150059). All media were supplemented with fetal bovine serum (FBS; Gemini Bio-products, catalogue No. 900–208) and penicillin-streptomycin solution (Corning, catalogue No. 30-002-Cl) at the final concentrations of 10% and 1%, respectively, unless stated otherwise. Cells were kept in a humidified incubator with 5% CO₂ at 37 °C. All cells were tested for mycoplasma infection (MycoAlert Mycoplasma Detection Kit, Lonza, catalogue No. LT07-218) and found to be negative. Cells were counted by means of Bright-Line^™ Hemacytometer (Sigma, catalogue No. Z359629).

Zaborowski M.P., Cheah P.S., Zhang X., Bushko I., Lee K., Sammarco A., Zappulli V., Maas S.L., Allen R.M., Rumde P., György B., Aufiero M., Schweiger M.W., Lai C.P., Weissleder R., Lee H., Vickers K.C., Tannous B.A, & Breakefield X.O. (2019). Membrane-bound Gaussia luciferase as a tool to track shedding of membrane proteins from the surface of extracellular vesicles. Scientific Reports, 9, 17387.

+ Open protocol

+ Expand

Ovarian Cancer Cell Line Cultivation

Check if the same lab product or an alternative is used in the 5 most similar protocols

The human ovarian cancer cell lines OVCAR-3, SKOV3, A2780, 3AO, COC1, OV-90, and human ovarian surface epithelial cells (HOSEpiC) were purchased from Type Culture Collection of Chinese Academy of Sciences (Shanghai, China). OVCAR-3, SKOV3, A2780, and COC1 cells were maintained in RPMI1640 medium (Corning, United States) supplemented with 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.), 100 U/ml penicillin and 100 μg/ml streptomycin (Thermo Fisher Scientific, Inc.) and cells were cultured at 37°C with 5% CO2. 3AO and OV-90 cells were maintained in Dulbecco’s Modified Eagle Medium (DMEM) (Corning, United States) supplemented with 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.), 100 U/ml penicillin and 100 μg/ml streptomycin (Thermo Fisher Scientific, Inc.). HOSEpiC cells were maintained in ovarian epithelial cell medium (ScienCell, United States).
Ketamine was supplied by Sigma-Aldrich (United States) and dissolved in DMSO.

Li T., Yang J., Yang B., Zhao G., Lin H., Liu Q., Wang L., Wan Y, & Jiang H. (2021). Ketamine Inhibits Ovarian Cancer Cell Growth by Regulating the lncRNA-PVT1/EZH2/p57 Axis. Frontiers in Genetics, 11, 597467.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!