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NP-KLH is a protein conjugate that can be used as a carrier protein for haptens in the production of antibodies. It is composed of Keyhole Limpet Hemocyanin (KLH) coupled to Nitrophenol (NP).

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Lab products found in correlation

Np klh, by Biosearch Technologies (3 mentions) Tamoxifen, by Merck Group (2 mentions) Immject alum, by Thermo Fisher Scientific (2 mentions) Np ficoll, by Biosearch Technologies (1 mentions) Sterile pbs, by Merck Group (1 mentions) Alum adjuvant, by Thermo Fisher Scientific (1 mentions) Matrigel, by Corning (1 mentions)

6 protocols using np klh

1

SRBC and NP-KLH Immunization Protocol

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Groups of animals were immunized with SRBC (Innovative Research) or NP-KLH (BioSearch Technologies). For SRBC immunization either a single intra-peritoneal injection of 1×109 SRBC or sequential SRBC injections (Day 0, 1–2×108; Day 5, 1×109) was used as indicated. For NP-KLH,100ug of NP-KLH 1:1 vol/vol in Alum (Immject Alum, Thermofisher) was precipitated and injected intraperitoneally.
Cumpelik A., Heja D., Hu Y., Varano G., Ordikhani F., Roberto M.P., He Z., Homann D., Lira S.A., Dominguez-Sola D, & Heeger P.S. (2021). Dynamic regulation of B cell Complement Signaling is Integral to Germinal Center Responses. Nature immunology, 22(6), 757-768.
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2

SRBC and NP-KLH Immunization Protocol

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Groups of animals were immunized with SRBC (Innovative Research) or NP-KLH (BioSearch Technologies). For SRBC immunization either a single intra-peritoneal injection of 1×109 SRBC or sequential SRBC injections (Day 0, 1–2×108; Day 5, 1×109) was used as indicated. For NP-KLH,100ug of NP-KLH 1:1 vol/vol in Alum (Immject Alum, Thermofisher) was precipitated and injected intraperitoneally.
Cumpelik A., Heja D., Hu Y., Varano G., Ordikhani F., Roberto M.P., He Z., Homann D., Lira S.A., Dominguez-Sola D, & Heeger P.S. (2021). Dynamic regulation of B cell Complement Signaling is Integral to Germinal Center Responses. Nature immunology, 22(6), 757-768.
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3

Conditional Genetic Manipulation of Immune Cells

Cited 2 times
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Prdm1gfp/+ 11 (link), Prdm1fl 25 (link), Irf4fl 26 (link), Xbp1fl 22 (link) and Rosa26-CreERT2 27 (link) mice were bred and maintained at the animal facilities of the Walter and Eliza Hall Institute. Prdm1fl mice carried a loxP-flanked exon 5, while Prdm1gfp mice expressed an allele truncated after exon 5 (of a total of 7 exons). The PCR analysis for exon 5 loss has been described previously25 (link). All mice were on C57BL/6 background. Animal experiments were conducted according to the protocols approved by the Walter and Eliza Hall Institute ethics committee. Immunization was a single intraperitoneal injection of 100 μg NP-KLH precipitated onto alum (ThermoScientific). For cell transfer experiments, total splenocytes (5 × 106) or isolated B cells (2 × 106) were injected intravenously into Rag1−/− recipients. Cre-mediated deletion of loxP-flanked alleles was triggered by the administration of tamoxifen (Sigma-Aldrich, 0.2 mg/g) by oral gavage on two consecutive days.
Tellier J., Shi W., Minnich M., Liao Y., Crawford S., Smyth G.K., Kallies A., Busslinger M, & Nutt S.L. (2016). Blimp-1 controls plasma cell function through regulation of immunoglobulin secretion and the unfolded protein response. Nature immunology, 17(3), 323-330.
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4

Conditional Genetic Manipulation of Immune Cells

Cited 2 times
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Prdm1gfp/+ 11 (link), Prdm1fl 25 (link), Irf4fl 26 (link), Xbp1fl 22 (link) and Rosa26-CreERT2 27 (link) mice were bred and maintained at the animal facilities of the Walter and Eliza Hall Institute. Prdm1fl mice carried a loxP-flanked exon 5, while Prdm1gfp mice expressed an allele truncated after exon 5 (of a total of 7 exons). The PCR analysis for exon 5 loss has been described previously25 (link). All mice were on C57BL/6 background. Animal experiments were conducted according to the protocols approved by the Walter and Eliza Hall Institute ethics committee. Immunization was a single intraperitoneal injection of 100 μg NP-KLH precipitated onto alum (ThermoScientific). For cell transfer experiments, total splenocytes (5 × 106) or isolated B cells (2 × 106) were injected intravenously into Rag1−/− recipients. Cre-mediated deletion of loxP-flanked alleles was triggered by the administration of tamoxifen (Sigma-Aldrich, 0.2 mg/g) by oral gavage on two consecutive days.
Tellier J., Shi W., Minnich M., Liao Y., Crawford S., Smyth G.K., Kallies A., Busslinger M, & Nutt S.L. (2016). Blimp-1 controls plasma cell function through regulation of immunoglobulin secretion and the unfolded protein response. Nature immunology, 17(3), 323-330.
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5

NP-Ficoll and NP-KLH Immunization in Mice

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NP-Ficoll (conjugation ratio 4:9) and NP-KLH (conjugation ratio 2:3, both from Biosearch Technologies) were dissolved in sterile PBS (Sigma). 25 μg of NP-Ficoll in 200 μl PBS or 100 μg of NP-KLH in 200 μl of PBS/alum adjuvant (mixed at a 1:1 ratio, from Thermo Fisher) was injected into mice intraperitoneally. Blood samples were collected into heparin-coated capillaries by tail vein punctures on indicated days. Plasma was separated by centrifugation and frozen. In certain experiments, the mice were sacrificed 7 days after immunization and tissues were collected for analyses.
Dunkel J., Viitala M., Karikoski M., Rantakari P., Virtakoivu R., Elima K., Hollmén M., Jalkanen S, & Salmi M. (2018). Enhanced Antibody Production in Clever-1/Stabilin-1–Deficient Mice. Frontiers in Immunology, 9, 2257.
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6

Subcutaneous Tumor Modeling and Immunology

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The subcutaneous tumor study was performed in NOD/SCIDγ mice (license PPL70/8586), by subcutaneously injecting 107 doxycycline- inducible shRNA- transduced cells with Matrigel (Corning). Tumor volume was calculated by the formula (1/2[length×width2 (link)]). Animals were administered 100 mg/mL doxycycline in drinking water and 0.2 mg/kg intraperitoneally.
Zbp1-/- animals18 (link) were obtained from Manolis Pasparakis, Institute of Genetics (Cologne, Germany). Ten- to 12-week-old littermates were immunized by intraperitoneal injection of 4 mg/kg NP-KLH (Santacruz Biotech) with Alum Adjuvant (Thermoscientific) at a 3:1 ratio followed by a booster dose of NPKLH on day 4. Serum NP-KLH-specific antibodies were measured.
Ponnusamy K., Tzioni M.M., Begum M., Robinson M.E., Caputo V.S., Katsarou A., Trasanidis N., Xiao X., Kostopoulos I.V., Iskander D., Roberts I., Trivedi P., Auner H.W., Naresh K., Chaidos A, & Karadimitris A. (2021). The innate sensor ZBP1-IRF3 axis regulates cell proliferation in multiple myeloma. Haematologica, 107(3), 721-732.
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