Skysacn 1276

Micro-CT analysis (SkySacn 1276, Bruker, Kontich, Belgium) was utilized to scan structural changes in the distal femur of mice. The scanned images were reconstructed using the SkyScan NRecon program and analyzed using SkyScan CTAn software (version 1.17.7.2, Bruker, Kontich, Belgium). The measured volume of the distal femur starting at 80 µm from the lower end of the growth plate and extending across 150 cross-sections (1.2 mm high). Bone morphometric parameters, including trabecular bone mineral density (BMD, g/cm³), trabecular bone volume fraction (BV/TV, %), trabecular number (Tb.N, mm), trabecular separation (Tb.Sp, mm), and trabecular thickness (Tb.Th, mm), were calculated.

The microarchitecture of the distal femur in the mice was scanned using micro-CT (SkySacn 1276, Bruker, Kontich, Belgium). SkyScan NRecon (version 1.7.42, Bruker) was used to reconstruct the images after scanning. The region of the femur commencing at a distance of 80 µm from the growth plate and extending across 150 cross-sections (0.8 μm section thickness) in the proximal direction was selected as the volume of interest using SkyScan CTAn (version 1.20.3.0, Bruker). Bone morphometric parameters including trabecular bone volume fraction (BV/TV, %), trabecular bone mineral density (BMD; g/cm³), trabecular number (Tb.N, mm), trabecular thickness (Tb.Th, mm), and trabecular separation (Tb.Sp, mm) were assessed.