Dc3l10

Manufactured by R&D Systems

Sourced in United States

The DC3L10 is a laboratory equipment product. It functions as a device for performing routine cell culture tasks.

Automatically generated - may contain errors

Lab products found in correlation

Human monoclonal antibodies, by Merck Group (1 mentions) Milliplex kit, by Merck Group (1 mentions) Ykl 40, by R&D Systems (1 mentions) Varioskan lux, by Thermo Fisher Scientific (1 mentions) 7600 analyzer, by Hitachi (1 mentions) Dplg70, by R&D Systems (1 mentions) Enzyme linked immunosorbent assay kit, by R&D Systems (1 mentions) Cobas c501, by Roche (1 mentions) Chi3l1, by R&D Systems (1 mentions) Dmp900, by R&D Systems (1 mentions) Lgals3bp, by R&D Systems (1 mentions) Mmp 9, by R&D Systems (1 mentions)

12 protocols using dc3l10

Multiplex Analysis of Plasma Adipokines and Inflammatory Markers

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Plasma adipokines (adiponectin, leptin and resistin), as well as inflammatory biomarkers interleukin (IL) 4, 5, 6, 8, 10, 13, tumor necrosis factor-alpha (TNF-α), monocyte chemoattractant protein 1 (MCP-1) were analyzed on a Luminex 200 system (Luminex Corporation, Austin, TX, USA) with human monoclonal antibodies (EMD Millipore Corp, Billerica, MA, USA) using MILLIplexTM kits (HADK1MAG-61K, HSTCMAG-258K, and HADK2MAG-61K) according to the manufacturer’s recommendations.
LPS-binding protein (LBP) and chitinase 3-like 1 (CHI3L1) levels were determined in plasma samples using CSB-EO9629H (CUSABIO TECHNOLOGY LLC, Houston, TX, USA) and DC3L10 (R&D Systems, InC, Minneapolis, MN, USA) ELISA kits, respectively, following the manufacturer’s instructions.
The coefficients of variation (CV) were 4.9, 8.0, 7.1, 6.3, 6.9, 10.5, 19.8, 20.8, 8.9, 15.2, 6.8, 10.4 and 11.5 for adiponectin, resistin, leptin, MCP-1, TNF-α, IL-10, 13, 4, 5, 6, 8, chitinase 3-like 1 and LBP, respectively.

Gomez-Llorente M.A., Martínez-Cañavate A., Chueca N., Rico M.D., Romero R., Anguita-Ruiz A., Aguilera C.M., Gil-Campos M., Mesa M.D., Khakimov B., Morillo J.A., Gil Á., Camacho J, & Gomez-Llorente C. (2020). A Multi-Omics Approach Reveals New Signatures in Obese Allergic Asthmatic Children. Biomedicines, 8(9), 359.

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Quantification of Serum CHI3L1 by ELISA

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Measurement of CHI3L1 was performed with the commercially available ELISA kit (Quantikine, R&D Systems, catalog DC3L10). The minimum detectable dose (MDD) of human CHI3L1 ranged from 1.25 to 8.15 pg/mL. The mean MDD was 3.55 pg/mL. Typical patient serum samples in healthy individuals can be detected in the range of 15.9 to 93.5 ng/mL. The experimental protocol followed Quantikine instructions except for the dilution of serum samples. A 1:500 dilution was used instead of a 1:50 dilution. The standard curve was created by GraphPad (GraphPad Software Inc.) using 4-parameter logistic curve fit. Serum CHI3L1 levels were measured in duplicate.

Sun X., Nakajima E., Norbrun C., Sorkhdini P., Yang A.X., Yang D., Ventetuolo C.E., Braza J., Vang A., Aliotta J., Banerjee D., Pereira M., Baird G., Lu Q., Harrington E.O., Rounds S., Lee C.G., Yao H., Choudhary G., Klinger J.R, & Zhou Y. (2022). Chitinase 3 like 1 contributes to the development of pulmonary vascular remodeling in pulmonary hypertension. JCI Insight, 7(18), e159578.

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Assessing Biomarkers for Cardiac Surgery-Associated AKI

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The CHI3L1 analysis was performed in-house. We measured the concentration of CHI3L1 by a sandwich enzyme-linked immunosorbent assay (ELISA) technique (DC3L10, R&D Systems, Minneapolis, MN, USA). Analyses performed externally were Cr and UNGAL. The Cobas c502 measured the concentration of Cr by a kinetic rate-blanked Jaffé assay (commercial reagents, Roche Diagnostics, Basel, Switzerland), whereas the Modular P measured the concentration of UNGAL by a particle-enhanced turbidimetric immunoassay (ST001-3CA, BioPorto, Hellerup, Denmark). All details were recently described [21 (link)], except for the standard sample dilution scheme used in the CHI3L1 ELISA, which is presented in Additional file 5: Table S2. For blood samples that were collected at routine collection times, a SCr concentration was already available in the hospital records. Based on the temporal relationship of the predictive value of UNGAL for CSA-AKI [19 (link)], we measured this biomarker at t1 and t3 only.
Besides UCHI3L1 and UNGAL, we also evaluated UCHI3L1 and UNGAL corrected for urine dilution by using the ratio to UCr as diagnostic test. Besides SCr, we also evaluated ΔSCr_tx-t0 as diagnostic test, representing the absolute change in SCr between SCr_tx and SCr_t0. The most recent SCr value recorded prior to surgery was considered as SCr_t0.

De Loor J., Herck I., Francois K., Van Wesemael A., Nuytinck L., Meyer E, & Hoste E.A. (2017). Diagnosis of cardiac surgery-associated acute kidney injury: differential roles of creatinine, chitinase 3-like protein 1 and neutrophil gelatinase-associated lipocalin: a prospective cohort study. Annals of Intensive Care, 7, 24.

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Biomarkers for Neurodegenerative and Demyelinating Disorders

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Glial fibrillary acidic protein (GFAP) and S100 calcium binding protein B (S100β), previously used to indicate neurodegeneration (Petzold et al., 2003 (link); Yardan et al., 2011 (link)), and PMS (Avsar et al., 2012 (link); Michetti et al., 2012 (link)) were measured in all samples with sufficient vCSF (27 PMS and 15 controls, 51% of samples) using western blot, with protein levels normalised to total protein level measured with BLOT-FastStain™. Chitinase 3 like 1 (CHI3L1), used to differentiate active and inactive PMS (Sellebjerg et al., 2017 (link)), and Chitinase 3 like 2 (CHI3L2), used to differentiate PMS and RRMS (Hinsinger et al., 2015 ), were measured by ELISA (Cusabio, YKL-40 and R&D systems DC3 L10, respectively) in the same samples as above (i.e. 27 PMS and 15 controls) as per manufacturers guidelines.

Lowes H., Pyle A., Duddy M, & Hudson G. (2019). Cell-free mitochondrial DNA in progressive multiple sclerosis. Mitochondrion, 46, 307-312.

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Quantifying Serum and BALF Biomarkers

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Human serum CHI3L1 levels (R&D Systems; Cat. #DC3 L10) and total (R&D Systems; Cat. #MB100B) and active (Biolegend, San Diego, CA; Cat. #437707) mouse TGF-β1 levels in mouse broncho-alveolar lavage fluid were measured using ELISA kits as directed by the manufacturer.

Geng B., Pan J., Zhao T., Ji J., Zhang C., Che Y., Yang J., Shi H., Li J., Zhou H., Mu X., Xu C., Wang C., Xu Y., Liu Z., Wen H, & You Q. (2018). Chitinase 3-like 1-CD44 interaction promotes metastasis and epithelial-to-mesenchymal transition through β-catenin/Erk/Akt signaling in gastric cancer. Journal of Experimental & Clinical Cancer Research : CR, 37, 208.

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Quantifying YKL-40 and VEGF by ELISA

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The serum concentrations of YKL‐40 and VEGF were quantified by corresponding ELISA kits (#DC3L10, #DVE00; R&D Systems, USA) according to the protocols. The absorbance at the wavelength of 450 nm was determined with the use of microplate reader (Varioskan + LUX; Thermo Fisher, USA) in the dark.

Bin Y., Liu Y., Jiang S, & Peng H. (2021). Elevated YKL‐40 serum levels may contribute to wet age‐related macular degeneration via the ERK1/2 pathway. FEBS Open Bio, 11(11), 2933-2942.

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Biomarker analysis in multiple sclerosis

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CSF and serum samples were stored at −80°C in the Biobank at the La Fe hospital and with the approval of the Ethics and Scientific Committees (PT17/0015/0043). CHI3L1 and NfL CSF levels were assessed as reported previously following consensus guidelines for CSF collection and biobanking.^{24 (link)} The mean intraassay coefficients of variation for NFL and CHI3L1 were 4.5% and 6.5%, respectively, and the interassay coefficients were 3.3% and 5.2%, respectively. We considered patients with biomarker levels more than 1.5 times the interquartile range (IQR) above the Q3 (CHI3L1 >367 ng/mL; NfL >2027 pg/mL) calculated in the global MS cohort as outliers. Serum CHI3L1 levels (sCHI3L1) were measured at a 1:50 dilution in 88 CSF samples using the same kit (DC3L10, R&D systems, Minneapolis, MN). To assess blood-brain barrier (BBB) permeability, the albumin index (Q-alb) was calculated for all the samples as the ratio of CSF (mg/L) to the serum albumin concentration (g/L).

Cubas-Núñez L., Gil-Perotín S., Castillo-Villalba J., López V., Solís Tarazona L., Gasqué-Rubio R., Carratalá-Boscá S., Alcalá-Vicente C., Pérez-Miralles F., Lassmann H, & Casanova B. (2021). Potential Role of CHI3L1+ Astrocytes in Progression in MS. Neurology® Neuroimmunology & Neuroinflammation, 8(3), e972.

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YKL-40 Serum ELISA Quantification

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Serum YKL-40 levels were measured with the related ELISA kits (R&D DC3L10) according to the manufacturer’s instructions.

Huan W., Yandong L., Chao W., Sili Z., Jun B., Mingfang L., Yu C, & Lefeng Q. (2021). YKL-40 Aggravates Early-Stage Atherosclerosis by Inhibiting Macrophage Apoptosis in an Aven-dependent Way. Frontiers in Cell and Developmental Biology, 9, 752773.

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Urinary Biomarkers for Kidney Injury

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Creatinine and urinary NGAL (UNGAL) analyses were performed externally. Creatinine concentrations were measured with a kinetic rate-blanked Jaffé assay (commercial reagents, Roche Diagnostics, Basel, Switzerland) on a Cobas c502, while UNGAL concentrations were measured with a particle-enhanced turbidimetric immunoassay (ST001-3CA, BioPorto, Hellerup, Denmark) on a Modular P. The concentration of CHI3L1 was determined in-house with a sandwich ELISA (DC3L10, R&D Systems, Minneapolis, MN, USA).
Both urinary CHI3L1 (UCHI3L1) and UNGAL concentrations were statistically analyzed as such and after correction for urine dilution by using the ratio to urinary creatinine (UCr). The relative change in SCr measured at enrollment was defined as the ratio of the enrollment SCr to reference SCr. The UO after enrollment, defined as the mean UO in the first valid 6-h period after enrollment, was determined as the mean of the 6 UO values that were calculated each h in the first valid 6-h period after enrollment.

De Loor J., Decruyenaere J., Demeyere K., Nuytinck L., Hoste E.A, & Meyer E. (2016). Urinary chitinase 3-like protein 1 for early diagnosis of acute kidney injury: a prospective cohort study in adult critically ill patients. Critical Care, 20, 38.

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Plasma YKL-40 Levels in Ischemic Stroke

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For patients with ischemic stroke, fasting blood samples were collected within 24 hours of hospital admission. Plasma was separated from the blood sample at the clinical laboratories of the participating hospitals and immediately frozen at −80 °C. We measured the plasma YKL‐40 concentrations of all participants centrally at Soochow University. The plasma YKL‐40 test was performed using a commercial enzyme‐linked immunosorbent assay kit (catalog number DC3L10; R&D Systems, Inc., Minneapolis, MN) according to the manufacturer's instructions. The intra‐ and interassay coefficients of variation were <5% and 7%, respectively. The laboratory technicians were blinded to the clinical characteristics and the clinical outcomes of the patients with ischemic stroke.

Xu T., Zhang K., Zhong C., Zhu Z., Zheng X., Yang P., Che B., Lu Y, & Zhang Y. (2022). Plasma Human Cartilage Glycoprotein‐39 Is Associated With the Prognosis of Acute Ischemic Stroke. Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease, 11(18), e026263.

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