STED microscopy was performed on a Leica TCS SP8 STED 3X confocal laser-scanning microscope equipped with a HC PL APO CS2 100x/1.40 OIL objective. Confocal sections were imaged with Leica LAS X Core software and processed with Huygens Software Suite (SVI). Images were recorded using 405 nm laser line at 1.4% laser power to image DAPI, and a 572 nm Laser line at 5.6% laser power to image Tubulin in confocal detector mode. LEM2 was imaged with a 653 nm laser line at 2.5% laser power in STED pulsed detector mode (gate start at 0.3 ns and gate end at 6.5 ns) with a Huygens saturation factor of 5.7. Deconvolved Images were contrast enhanced in ImageJ (NIH), raw data available upon request.
Apo cs2 100x 1.40 oil objective
Manufactured by Leica
The APO CS2 100x/1.40 OIL objective is a high-performance microscope objective designed for advanced microscopy applications. It features an apochromatic correction and a numerical aperture of 1.40, which provides excellent image quality and resolution. The objective is optimized for use with oil immersion.
Automatically generated - may contain errors
2 protocols using apo cs2 100x 1.40 oil objective
1
High-resolution STED Imaging of Cellular Structures
2
High-resolution STED Imaging of Cellular Structures
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