Embryonic stem cell qualified fbs

MSCs were isolated as previously described^{27 (link),42 (link)}. Briefly, adipose tissues were minced using scissors and washed with phosphate-buffered saline (PBS) (Thermo Fisher Scientific, Waltham, MA, USA) and were then enzymatically dissociated with 1 ml of 0.1% collagenase (type I) (Wako, Osaka, Japan) in PBS for 1 h at 37 °C. The dissociated tissue was incubated in α-minimum essential medium (αMEM) (Thermo Fisher Scientific) supplemented with foetal bovine serum (FBS) (Thermo Fisher Scientific), and centrifuged. The cell pellet was resuspended in PBS. After centrifugation, adipose cells including MSCs were obtained. Luc+ MSCs were obtained from adipose tissues of luciferase transgenic rats^{6 (link),43 (link)}. Isolated cells were cultured in αMEM supplemented with 20% embryonic stem cell-qualified FBS (Thermo Fisher Scientific).

All cell lines used in this study originate from E14TG2a.4 mESC (mouse Embryonic Stem Cells, Mus musculus strain 129/Ola, male) with integrated gene targeting vector pAW2 at the ROSA26 locus to enable recombinase-mediated cassette exchange (Zhou et al., 2013 (link)). mESC cells were grown in G-MEM medium (ThermoFisher) supplemented with 5% embryonic stem cell-qualified FBS (ThermoFisher), 5% Knockout Serum Replacement (ThermoFisher), 1x non-essential amino acids (ThermoFisher), 1x sodium pyruvate (ThermoFisher), 100 μM β-mercaptoethanol (Sigma-Aldrich) and 1000 U/mL LIF (Millipore). Cells were sub-cultured every two days into dishes coated with 0.1% gelatine and kept at 37°C in a 5% CO₂ water-saturated incubator.
For depletion of ARID1A, cells were seeded at ∼20,000 cells per cm² and 500 μM auxin analog 1-naphthaleneacetic acid (NAA, Sigma-Aldrich) in 1M sodium hydroxide was added for the indicated time before harvesting the cells after two days of growing.