Western blotting was performed as described previously.24 (link)–26 (link) Monoclonal antibody (mAb) against glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibody (G-9) (sc-365062) was used to standardize cytosolic protein loading on the sodium dodecyl sulfate-polyacrylamide gel electrophoresis. ROS1 (D4D6) rabbit mAbs from Cell Signaling Technology (catalog no: 3287) were used to recog-nize FIG-ROS1 and Phospho-ROS1 activity (Tyr2274) (catalog no: 3078). Anti-GOPC/FIG antibody (MyBioSource: MBS421395) was used to detect FIG expression. p-Met antibody (Tyr 1365) (sc-3408), p-Met (F-5) (sc-377548), p-ALK antibody (Tyr 1586) (sc-109905), Bim (H-191) (sc-11425), survivin (D-8) (sc-17779), Mcl-1 antibody (S-19) (sc-819), and p-Akt1/2/3 antibody (Ser 473)-R (sc-7985-R) were purchased from Santa Cruz Biotechnology, Inc. The secondary antibodies were horseradish peroxidase-conjugated goat anti-mouse immunoglobulin (Ig) G (ICN Biomedicals) and horseradish peroxidase-conjugated goat anti-rabbit IgG (ICN Biomedicals).
P akt1 2 3 antibody ser 473 r sc 7985 r
Manufactured by Santa Cruz Biotechnology
Sourced in United Kingdom
The P-Akt1/2/3 antibody (Ser 473)-R (sc-7985-R) is a polyclonal antibody that recognizes the phosphorylated forms of Akt1, Akt2, and Akt3 at serine 473. This antibody is designed for use in various immunological techniques such as Western blotting, immunoprecipitation, and immunohistochemistry.
Automatically generated - may contain errors
Lab products found in correlation
Phospho ros1, by Cell Signaling Technology (1 mentions)
Horseradish peroxidase conjugated goat anti mouse immunoglobulin ig g, by ICN Biomedicals (1 mentions)
Horseradish peroxidase conjugated goat anti rabbit igg, by ICN Biomedicals (1 mentions)
Crizotinib, by Selleck Chemicals (1 mentions)
Total erk1 2, by Cell Signaling Technology (1 mentions)
Trastuzumab, by Roche (1 mentions)
2 protocols using p akt1 2 3 antibody ser 473 r sc 7985 r
1
Western Blotting Methodology in ROS1 Signaling
2
Antibody-based Signaling Pathway Analysis
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Anti-cMet(ab51067) and anti-Her2(ab134182) antibodies were from Abcam (Cambridge, UK), p-Met antibody (Tyr 1365) (sc-3408), p-ALK antibody (Tyr 1586) (sc-109905), p-Akt1/2/3 antibody (Ser 473)-R (sc-7985-R) and horseradish peroxidase-conjugated secondary antibodieswere purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, USA). Phospho-FGFR (tyr653/654,#3471), phosphor-p44/p42 MAPK (Erk1/2, #4370), total-Erk1/2 (#4960) were purchased from Cell Signaling Technology. Trastuzumab was obtained from Roche, Inc. (Roche, USA), while crizotinib and ADZ4547 were from Selleck Chemicals, LLC (Houston, CA, USA).
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