Cupric nitrate trihydrate

Tetraethoxysilane (TEOS), ammonium hydroxide (NH₃·H₂O, 28%), cupric nitrate trihydrate (Cu(NO₃)₂·3H₂O), and lipopolysaccharide (LPS) were purchased from Sigma-Aldrich (Shanghai, China), Cefoperazone (CFP, CAS: 62893-19-0) was purchased from Macklin. All reagents were applied as received with no further purification.
BALB/c mice (male, 20–25 g) were provided by the Laboratory Animals Department of the Army Medical University (AMU, Chongqing, China). All animal experiments were performed according to ethical standards and with the approval of AMU's Institutional Animal Care and Use Committee. The experimental mice were housed individually in plastic cages with free access to autoclaved standard rodent chow and water under standard conditions (Room temperature: 25 °C; relative humidity: 50%; circadian rhythm: 12 h). All mice were fed adaptively for 1 week before the experiment.
Multi-drug resistant Pseudomonas aeruginosa (P. aeruginosa, ATCC 27853) was provided by the Clinical Microbiology Laboratory, Institute of Burn Research, Southwest Hospital, AMU (Chongqing, China). NIH 3T3 cells and RAW264.7 cells were provided from the Chinese Academy of Science Cell Bank for Typical Culture Collection.

Complete EDTA-free protease inhibitor cocktail was purchased from Roche Diagnostics (Laval, QC, Canada), and ProLong gold antifade reagent with 4′,6-diamidino-2-phenylindole (DAPI), SlowFade gold reagent mounting medium, the cell culture media Dulbecco’s modified Eagle’s medium (DMEM) and RPMI 1640, and fetal bovine serum (FBS) were purchased from Invitrogen (Carlsbad, CA).
Lipofectamine 2000 and Lipofectamine RNAiMAX were purchased from Invitrogen (Carlsbad, CA), and PerFectin transfection reagent was obtained from Genlantis (San Diego, CA). Pooled siRNAs against β-TrCP1 (hs.Ri.BTRC.13) and β-catenin (hs.Ri.CTNNB1.13) or individual siRNAs against TCF-4 (hs.Ri.TCF4.13.1 and hs.Ri.TCF4.13.2) were purchased from IDT (Coralville, IA).
Reagents for the purification and quantitation of miRNAs, including the miRNeasy minikit, the miScript PCR starter kit, the miScript II RT kit, and the miScript SYBR green PCR kit, were purchased from Qiagen (Toronto, ON, Canada).
Chemicals for nonesterified fatty acid (NEFA) extraction, including cupric nitrate trihydrate, triethanolamine, and a mixture of dicarbazone-dicarbazide, were purchased from Sigma (Oakville, Ontario, Canada).