Lung tissue was sliced and dewaxed to water (xylene I 15 min, xylene II 15 min, xylene III 15 min, absolute ethanol I 5 min, absolute ethanol II 5 min, 85% alcohol 5 min, 75% alcohol 5 min, and distilled water). After repairing with citric acid antigen repair buffer, add 3% hydrogen peroxide to incubate. After blocking at room temperature for 30 minutes and adding rabbit polyclonal anti-p-JAK1 (1 : 200 vol/vol, Abcam), anti-p-STAT1 (1 : 100 vol/vol, Abcam), and anti-SOCS3 (1 : 100 vol/vol, Abcam), put it in a wet box at 4°C refrigerator overnight. After washing with PBS, adding secondary antibody (goat anti-rabbit) (1 : 200), DAB color development, and observing under the microscope, the reaction was terminated when it showed brown. Then, hematoxylin counterstained and returned to blue, and the neutral resin sealed.
Anti p stat1
Manufactured by Abcam
Sourced in United States
Anti-p-STAT1 is a primary antibody that recognizes the phosphorylated form of STAT1, a transcription factor involved in the JAK-STAT signaling pathway. This antibody can be used to detect and quantify the levels of activated STAT1 in various cell and tissue samples.
Automatically generated - may contain errors
Lab products found in correlation
Anti p jak1, by Abcam (2 mentions)
Anti stat1, by Abcam (2 mentions)
Anti gapdh, by Abcam (2 mentions)
Anti socs3, by Abcam (1 mentions)
Polyvinylidene fluoride membrane, by Merck Group (1 mentions)
Anti stat1, by Proteintech (1 mentions)
Anti gapdh, by Yeasen (1 mentions)
Anti jak1, by Proteintech (1 mentions)
Anti cxcl10, by Abcam (1 mentions)
Anti tublin, by Yeasen (1 mentions)
Rabbit peroxidase conjugated secondary antibody, by ABclonal (1 mentions)
Anti pd l1, by Proteintech (1 mentions)
Anti phosphorylated jak2, by Abcam (1 mentions)
Anti jak2, by Cell Signaling Technology (1 mentions)
Chemidoc xrs imaging system, by Bio-Rad (1 mentions)
Anti jak1, by Abcam (1 mentions)
Anti stat2, by Abcam (1 mentions)
Immobilob western chemiluminescent hrp substrate, by Merck Group (1 mentions)
4 protocols using anti p stat1
1
Immunohistochemical analysis of lung tissue
2
Western Blot Profiling of HPV Biomarkers
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Cultured cells were lysed with lysis buffer. Equal amounts of protein were run on 10% SDS-PAGE and electrotransferred to polyvinylidene fluoride membranes (Millipore). After blocking with 5% milk in TBST, membranes were incubated with primary antibodies overnight. The following antibodies were used: anti-HPVE6 (1:1000, Arigo), anti-HPVE7(1:1000, Bioss), (anti-CXCL10 (1:1,000, Abcam), anti-CXCR3 (1:1,000, Boster), anti-PDL1 (1:2,000, proteintech), anti-STAT1 (1:1,000, proteintech), anti-pSTAT1 (1:1,000, Abcam), anti-JAK1 (1:2,000, proteintech), anti-GAPDH (1:6,000, Yeasen) and anti-tublin (1:3,000, Yeasen). Membranes were then incubated with the rabbit peroxidase-conjugated secondary antibody (1:10,000, Abclonal). The blots were detected by sensitive chemiluminescence liquid analysis (Yeasen) and Biorad software was used to capture the images.
3
Western Blot Analysis of PD-L1, JAK2, and STAT1
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Total protein from HTC116 cell lines was extracted using a mammalian protein extraction kit (cat. no. CW0891M; CoWin Biosciences) and used according to the manufacturer's instructions. Total protein was quantified using a bicinchoninic acid assay kit (cat. no. CW0014S; CoWin Biosciences). The mass of protein loaded per lane were 10–40 µg on a 12.5% SDS-PAGE gel. The proteins were transferred to PVDF membranes. The membranes were blocked with 5% no-fat milk at room temperature for 1 h. The membranes were incubated with the following primary antibodies overnight at 4°C: Anti-PD-L1 (1:2,000; cat. no. 13684; Cell Signaling Technology, Inc.), anti-JAK2 (1:2,000; cat. no. ab39636; Abcam), anti-phosphorylated (p)-JAK2 (1:2,000; cat. no. ab32101; Abcam), anti-STAT1 (1:2,000; cat. no. ab31369; Abcam), anti-p-STAT1 (1:1,000; cat. no. ab109461; Abcam) and anti-GAPDH (1:1,000; cat. no. ab181602; Abcam). The membranes were incubated with an HRP-conjugated goat anti-rabbit IgG secondary antibody at room temperature for 25 min (1:10,000; cat. no. ab6721; Abcam). Protein bands were visualized using ECL reagents (iBright cat. no. CL750; Thermo Fisher Scientific, Inc.) and a ChemiDoc XRS + imaging system (Bio-Rad Laboratories, Inc.).
4
TNBC Cell Protein Analysis
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Proteins extracted from TNBC cells (30 µg) were separated by SDS-PAGE, and electro-transferred onto a PVDF membrane. Membranes were blocked with 5% skimmed milk powder, and then incubated overnight with primary antibodies: anti-TMEM2 (1:1500, Abcam, Burlingame, CA, USA), anti-JAK1 and anti-p-JAK1 (1:2000, Abcam), anti-STAT1 and anti-p-STAT1 (1:2500, Abcam), anti-STAT2, anti-p-STAT2 and anti-GAPDH (1:3000, Abcam) at 4°C. Following incubation with secondary antibody (1:5000; Abcam), the immunoreactivities were detected by Immobilob ™ Western Chemiluminescent HRP Substrate (Millipore, Billerica, MA, USA).
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