The circular birefringence was measured on a P-2000 Digital Polarimeters. HR-ESI-MS spectrum was measured on an X500 QTOF mass spectrometer (Sciex, USA). IR spectra were measured on an Agilent 6530 Accurate-Mass spectrometer. The 1D and 2D NMR including 1H-(500 MHz), and 13C-(125 MHz) NMR spectra were employed using AVANCE III HD 500 FT-NMR spectrometer (Bruker, Corp., Germany). CD spectrum was achieved using Applied Photophysics Chirascan spectropolarimeter. Column chromatography (CC) was performed using silica gel (60F245, and RP-18 F254s. Merck, Germany), Sephadex LH-20 (Sigma-Aldrich, USA), and Thin Layer Chromatography (TLC) was performed on silica gel 60 F254 [23 (link)]. The identified compounds were monitored under a UV lamp at 254 or 365 nm and heated immediately after spraying with 10 % H2SO4.
X500 qtof mass spectrometer
Manufactured by AB Sciex
Sourced in United States
The X500 QTOF mass spectrometer is a high-resolution quadrupole time-of-flight (QTOF) mass spectrometer designed for precise and accurate mass analysis. It utilizes a quadrupole mass analyzer and a time-of-flight mass analyzer to provide high-resolution, high-mass-accuracy mass measurements.
Automatically generated - may contain errors
Lab products found in correlation
Avance 3 hd 500 ft nmr spectrometer, by Bruker (2 mentions)
Exionlc system, by AB Sciex (2 mentions)
Spectrum two ft ir spectrometer, by PerkinElmer (1 mentions)
Avance 3 spectrometer, by Bruker (1 mentions)
Chemstation software, by Agilent Technologies (1 mentions)
Inertsustain c18, by GL Sciences (1 mentions)
Rp 18 f254s plates, by Merck Group (1 mentions)
S 4800 scanning electron microscope, by Hitachi (1 mentions)
Chirascan spectrometer, by Applied Photophysics (1 mentions)
Agilent 1200 system, by Agilent Technologies (1 mentions)
P 2000 polarimeter, by Jasco (1 mentions)
Avance 500 mhz, by Bruker (1 mentions)
7 protocols using x500 qtof mass spectrometer
1
Spectroscopic Characterization of Natural Compounds
2
UHPLC-Q-TOF-MS/MS Metabolomics Analysis
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The UHPLC-Q-TOF-MS/MS system consisted of two ExionLC AD pumps and an ExionLC AD autosampler coupled with a high resolution X500 Q-TOF mass spectrometer (Sciex, USA). The SCIEX OS 1.0 software from Sciex (Sciex, USA) contains instrument control, data acquisition, data processing, and reporting functionality, all in the one package. Chromatographic separation was achieved on a Agilent SB-C18 RRHD column (100 mm × 3.0 mm, 1.8 μm) (Agilent Technologies, USA). All centrifugation were performed on a Sigma 3–30 K refrigerated centrifuge (Sigma, Germany). Ultrasonic process was operated on a KQ-300 GDV Thermostat Ultrasonic Instrument (Kunshan, China).
3
Microwave-Assisted Organic Synthesis
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All chemicals were used as received without any further purification and obtained from Aldrich or Merck. Microwave irradiation experiments were performed using an Anton Paar Microwave Synthetic Reactor Monowave 400. Merck silica gel 60 F254 plates and Merck silica gel 60 (240–400 mesh) were used for thin-layer chromatography and column flash chromatography, respectively. Melting points were measured in open capillaries on a Buchi melting point B-545 apparatus (Buchi Instrument, Switzerland) and the values reported are uncorrected. A SCIEX X500 QTOF mass spectrometer in ESI (+) or ESI (−) mode was used to calculate the HRMS spectra. IR spectra have been recorded as KBr pellets, with a PerkinElmer Spectrum Two FT-IR spectrometer. NMR experiments were acquired using a Bruker Avance III spectrometer (600 and 125/150 MHz).
4
Epitope Peptide Mapping by LC-UV and LC-MS
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LC-UV separations were performed on Agilent HPLC series 1100 and 1200 systems (Santa Clara, CA, USA), equipped with a mobile-phase online degasser, a quaternary pump, an autosampler, a column thermostated compartment, and a diode array detector, and controlled by an Agilent ChemStation software (Rev. B.04.01).
For the epitope peptide mapping study, the chromatographic separations were performed on an ExionLC system (SCIEX, Framingham, MA, USA) equipped with a mobile-phase online degasser, a quaternary pump, an autosampler, and a column thermostated compartment. MS detection was carried out by an X500 QTOF mass spectrometer (SCIEX) with an ESI source. The LC–MS system was controlled by a SCIEX OS software (1.7 version).
For the epitope peptide mapping study, the chromatographic separations were performed on an ExionLC system (SCIEX, Framingham, MA, USA) equipped with a mobile-phase online degasser, a quaternary pump, an autosampler, and a column thermostated compartment. MS detection was carried out by an X500 QTOF mass spectrometer (SCIEX) with an ESI source. The LC–MS system was controlled by a SCIEX OS software (1.7 version).
5
Extraction and LC-MS Analysis of Metabolites
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Freshly prepared ethyl acetate and n-hexane samples were prepared by dissolving the extract in the solvent. Samples were filtered through a 0.45 μm membrane filter (cellulose acetate (CA) membranes made of entirely cellulose acetate polymer, Sterlitech, Auburn, WA, USA). All samples were prepared and used immediately for analysis. Extracts were analyzed using the Exion LC system (SCIEX, Framingham, MA, USA), fitted with an X500 QTOF mass spectrometer (SCIEX) equipped with an ESI. Separation was performed on an InertSustain C18 (GL Sciences Inc., Tokyo, Japan, 25 cm × 4.6 mm × 5 m). The mobile phase was with (A) 0.1% formic acid in water (1:1000) and (B) acetonitrile with a gradient flow rate of 1.0 mL/min. The injection volume was set to 0.6 μL. Positive-mode LC-MS was performed for which the ion spray voltage was 5000 V, the declustering potential was 80 V, and the collision energy was 10 V.
6
Spectroscopic Characterization of Natural Compounds
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Optical rotations were determined on a JASCO P-2000 polarimeter (Tokyo, Japan). HR-IDA-TOF-MS were recorded on a X500 QTOF mass spectrometer (Sciex, USA). Circular dichroism (CD) spectra were acquired on a Chirascan spectrometer (Applied Photophysics, UK). The 1 H (500 MHz) and 13 C NMR (125 MHz) spectra were recorded on an AVANCE III HD 500 FT-NMR spectrometer (Bruker, Germany) with tetramethylsilane (TMS) as an internal standard. Column chromatography (CC) was performed on silica gel (Kieselgel 60, 70-230 mesh and 230-400 mesh, Merck, Darmstadt, Germany) and YMC*GEL (ODS-A, 12 nm, S-150 m, YMC Co., Ltd., Japan) resins. TLC used pre-coated silica gel 60 F 254 (Merck) and RP-18 F 254S plates (Merck), and compounds were visualised by spraying with aqueous 10% H2SO4 and heating for 3-5 min. Preparative high performance liquid chromatography (HPLC) was performed on an Agilent 1200 system (Agilent Technologies, USA) equipped with a G1361A preparative pump, a G1315D diode array detector, a G2260A preparative autosampler, and a COSMOSIL 5C 18 -MS-II Packed column (250 × 10 mm, 5 m). images taken using a Hitachi S-4800 scanning electron microscope.
7
Synthesis and Characterization of Novel Organic Compounds
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All chemicals were obtained from commercial sources and were used without further purification. The melting points were determined in open capillaries and are uncorrected. The IR spectra were recorded on an FT-IR Shimadzu 8400-S. NMR spectra were measured on a Bruker Avance 500 MHz in DMSO-d 6 using residual solvent DMSO-d 6 signals as the internal references. Mass spectra were recorded on a Sciex X500 QTOF mass spectrometer.
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