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Pc hp 845x uv visible system

Manufactured by Agilent Technologies
Sourced in Germany

The PC-HP 845x UV-Visible System is a laboratory equipment product designed for spectrophotometric analysis. It is capable of measuring the absorbance or transmittance of light in the ultraviolet and visible light spectrum.

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2 protocols using pc hp 845x uv visible system

1

Soxhlet Extraction and UV-Vis Polyphenol Analysis

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A Soxhlet apparatus was used for drug extraction. The quantitative analysis of the polyphenolic substances was performed with an Agilent 8453 UV/Vis spectrophotometer (Agilent, Germany) equipped with PC -HP 845x UV—Visible System (Agilent, Germany) and 1 cm quartz cuvettes.
With the exception of the Folin–Ciocalteu phenol reagent (FCR), casein (Merck, Darmstadt, Germany) and quercetin (Roth, Karlsruhe, Germany) all chemicals and reagents for polyphenol analysis were of analytical quality grade and were supplied by Kemika (Zagreb, Croatia).
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2

UV-Vis Spectrophotometric Sample Analysis

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Water-bath, reflux condenser, UV/Vis spectrophotometer Agilent 8453 (Agilent, Karlsruhe, Germany) with PC-HP 845x UV-Visible System (Agilent, Karlsruhe, Germany) and 1 cm quartz cells were used for all absorbance measurements. Filtration of prepared sample solutions was performed by using 0.20 µm Minisart-plus membrane filter (Sartorius AG, Goettingen, Germany).
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