The amount of lactose in the samples was determined using HPLC method as described by Hou et al. with some modifications [20 ]. One mL of sample was centrifuged at 13000 rpm for 10 min. The clear fraction was filtered through a 0.2 μm nylon membrane filter and injected onto HPLC system (Alliance 2690/5: Waters Corporation, Milford, CA), equipped with a 4.6 mm × 150 mm Agilent Zorbax Carbohydrate Analysis column (Agilent Technologies Inc., USA). The mobile phase used is 75% (v/v) acetonitrile (Fisher, HPLC grade). The flow rate was set at 1.4 mL min−1 and the analysis was carried out at 30°C using a refractive index detector (RI-1371, Waters Corporation, Milford, CA).
Alliance 2690 5
Manufactured by Waters Corporation
Sourced in United States
The Alliance 2690/5 is a high-performance liquid chromatography (HPLC) system designed for analytical and preparative applications. It features a modular design with a range of available components, including a solvent delivery module, an autosampler, a column compartment, and a variety of detection modules. The core function of the Alliance 2690/5 is to separate, identify, and quantify components within a sample mixture.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using alliance 2690 5
1
Lactose Quantification by HPLC
2
Amino Acid Analysis of Grains
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
EMBRAPA Food Technology performed the extraction and analysis of amino acids from grains. The analysis of amino acids was done on the lyophilized flour produced for protein extraction. It was done using a liquid chromatograph, model Alliance 2690/5, with column oven and fluorescence detector 2475 (Waters, USA), with a chromatographic Symmetry C18 3.5 µm column (4.6x75mm). The measurements were done according to the methods proposed by AOAC 994.12/2000, as previously described (Liu et al., 1995 ).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!