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Rabbit anti hoxa9 07 178

Manufactured by Merck Group
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Rabbit anti-Hoxa9 (07-178) is a laboratory reagent used for research purposes. It is an antibody that specifically binds to the Hoxa9 protein, which is a transcription factor involved in various cellular processes. The antibody can be used in techniques such as Western blotting, immunoprecipitation, and immunohistochemistry to detect and study the Hoxa9 protein.

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Lab products found in correlation

Mouse anti mll1, by Merck Group (2 mentions) Sc 304, by Santa Cruz Biotechnology (2 mentions) Alexa fluor 488 anti rabbit igg, by Thermo Fisher Scientific (2 mentions) Anti mouse igg alexa fluor 594, by Thermo Fisher Scientific (2 mentions) Anti mouse igg1 alexa fluor 594, by Thermo Fisher Scientific (2 mentions)

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Anti-Hoxa9 Rabbit anti-

2 protocols using rabbit anti hoxa9 07 178

1

Immunofluorescence Analysis of Satellite Cells

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Freshly isolated SCs were allowed to settle on poly-L-lysine coated diagnostic microscope slides for 30 min at RT. All cells and myofibers were fixed with 2% PFA/PBS, permeabilized with 0.5% Triton X-100/PBS and blocked with 10 % Horse Serum/PBS for 1h at RT. Cells and fibers were stained with primary antibodies in blocking solution overnight at 4°C. Samples were washed three times with PBS and incubated with secondary antibodies for 1h at RT. Nuclei were counterstained with DAPI/PBS. Cultured cells were kept in PBS; freshly isolated SCs and myofibers were mounted with Permaflour. The following primary antibodies were used: undiluted mouse anti-Pax7 (DSHB), 1:300 rabbit anti-Hoxa9 (07-178, Millipore), 1:500 mouse anti-MLL1 (05-765, Millipore), 1:500 rabbit anti-WDR5 (A302-429A, Bethyl Laboratories), 1:300 rabbit anti-H3K4me3 (C15410003-50, Diagenode), 1:200 rabbit anti-MyoD (sc-304, Santa Cruz). The following secondary antibodies were used at 1:1,000 concentration: anti-rabbit IgG Alexa-Fluor 488, anti-mouse IgG Alexa-Fluor 594, anti-mouse IgG1 Alexa-Fluor 594 (Life Technologies).
Schwoerer S., Becker F., Feller C., Baig A.H., Koeber U., Henze H., Kraus J.M., Xin B., Lechel A., Lipka D.B., Varghese C.S., Schmidt M., Rohs R., Aebersold R., Medina K.L., Kestler H.A., Neri F., von Maltzahn J., Tuempel S, & Rudolph K.L. (2016). Epigenetic stress responses induce muscle stem cell aging by Hoxa9 developmental signals. Nature, 540(7633), 428-432.
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2

Immunofluorescence Analysis of Satellite Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Freshly isolated SCs were allowed to settle on poly-L-lysine coated diagnostic microscope slides for 30 min at RT. All cells and myofibers were fixed with 2% PFA/PBS, permeabilized with 0.5% Triton X-100/PBS and blocked with 10 % Horse Serum/PBS for 1h at RT. Cells and fibers were stained with primary antibodies in blocking solution overnight at 4°C. Samples were washed three times with PBS and incubated with secondary antibodies for 1h at RT. Nuclei were counterstained with DAPI/PBS. Cultured cells were kept in PBS; freshly isolated SCs and myofibers were mounted with Permaflour. The following primary antibodies were used: undiluted mouse anti-Pax7 (DSHB), 1:300 rabbit anti-Hoxa9 (07-178, Millipore), 1:500 mouse anti-MLL1 (05-765, Millipore), 1:500 rabbit anti-WDR5 (A302-429A, Bethyl Laboratories), 1:300 rabbit anti-H3K4me3 (C15410003-50, Diagenode), 1:200 rabbit anti-MyoD (sc-304, Santa Cruz). The following secondary antibodies were used at 1:1,000 concentration: anti-rabbit IgG Alexa-Fluor 488, anti-mouse IgG Alexa-Fluor 594, anti-mouse IgG1 Alexa-Fluor 594 (Life Technologies).
Schwoerer S., Becker F., Feller C., Baig A.H., Koeber U., Henze H., Kraus J.M., Xin B., Lechel A., Lipka D.B., Varghese C.S., Schmidt M., Rohs R., Aebersold R., Medina K.L., Kestler H.A., Neri F., von Maltzahn J., Tuempel S, & Rudolph K.L. (2016). Epigenetic stress responses induce muscle stem cell aging by Hoxa9 developmental signals. Nature, 540(7633), 428-432.
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