Evos fl color imaging system

Manufactured by Olympus

The EVOS® FL Color Imaging System is a compact, high-performance fluorescence microscope designed for live-cell imaging and analysis. It features LED illumination, advanced optics, and a high-resolution color camera to capture detailed images of fluorescently labeled samples.

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Lab products found in correlation

Dp71 camera, by Olympus (2 mentions) Neutral buffered formalin, by Bio-Optica (2 mentions) Analysis image processing software, by Olympus (2 mentions)

2 protocols using evos fl color imaging system

Histological Assessment of Lung Pathology

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For histology, lungs were removed and immediately fixed in 10% neutral buffered formalin (Bio-optica) for 24 h. Lungs were then dehydrated, embedded in paraffin, sectioned into 3–4 μm slices and stained with periodic acid-Schiff reagent (PAS), Alcian blue reagent or Masson reagent (Bio-optica). All images were visualized using a BX51 Olympus equipped with a high-resolution DP71 camera (Olympus) with a × 4, × 20 and × 40 objective with the analySIS image processing software (Olympus) or EVOS® FL Color Imaging System with a × 40 objective. The pathology scale considered both the severity and the distribution of morphologic changes within the lungs (Hubbs et al., 1997 (link)). The total pathology score is the sum of the severity and the distribution and potentially range from 0 to 10. The severity scores are: none = 0; minimal = 1; mild = 2; moderate = 3; marked = 4; severe = 5. The distribution scores are: none = 0; focal = 1; locally extensive = 2; multifocal = 3; multifocal and coalescent = 4; diffuse = 5 (Hubbs et al., 1997 (link)).

Zelante T., Choera T., Beauvais A., Fallarino F., Paolicelli G., Pieraccini G., Pieroni M., Galosi C., Beato C., De Luca A., Boscaro F., Romoli R., Liu X., Warris A., Verweij P.E., Ballard E., Borghi M., Pariano M., Costantino G., Calvitti M., Vacca C., Oikonomou V., Gargaro M., Wong A.Y., Boon L., den Hartog M., Spáčil Z., Puccetti P., Latgè J.P., Keller N.P, & Romani L. (2021). Aspergillus fumigatus tryptophan metabolic route differently affects host immunity. Cell Reports, 34(4), 108673.

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Histological Analysis of Spinal Cords

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For histology, spinal cords were removed and immediately fixed in 10% neutral buffered formalin (Bio-optica) for 24 h. Tissues were then dehydrated, embedded in paraffin, sectioned into 3–4 μm slices and stained with hematoxylin and eosin stain. All images were visualized using a BX51 Olympus equipped with a high-resolution DP71 camera (Olympus) with a × 4, × 20 and × 40 objective with the analySIS image processing software (Olympus) or EVOS® FL Color Imaging System with a × 40 objective.

Zelante T., Paolicelli G., Fallarino F., Gargaro M., Vascelli G., De Zuani M., Fric J., Laznickova P., Kohoutkova M.H., Macchiarulo A., Dolciami D., Pieraccini G., Gaetani L., Scalisi G., Trevisan C., Frossi B., Pucillo C., De Luca A., Nunzi E., Spaccapelo R., Pariano M., Borghi M., Boscaro F., Romoli R., Mancini A., Gentili L., Renga G., Costantini C., Puccetti M., Giovagnoli S., Ricci M., Antonini M., Calabresi P., Puccetti P., Di Filippo M, & Romani L. (2024). A microbially produced AhR ligand promotes a Tph1-driven tolerogenic program in multiple sclerosis. Scientific Reports, 14, 6651.

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