15n5 8 oxodg

Manufactured by Cambridge Isotopes

Sourced in United States

15N5-8-oxodG is a stable isotope-labeled compound used as an internal standard in analytical procedures. It is a modified guanine nucleoside with five 15N atoms and an 8-oxo group. This compound is utilized in the quantitative analysis of 8-oxo-2'-deoxyguanosine, a biomarker for oxidative DNA damage.

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Lab products found in correlation

8 oxodg, by Merck Group (2 mentions) Ultrapure water, by Avantor (1 mentions) Lc ms grade acetonitrile, by Merck Group (1 mentions) β glucuronidase escherichia coli, by Roche (1 mentions) Sodium chloride (nacl), by Merck Group (1 mentions) Toluene, by Merck Group (1 mentions) Milli q integral 5 system, by Merck Group (1 mentions) Formic acid, by Merck Group (1 mentions) Milli q integral system, by Merck Group (1 mentions) Amicon ultracentrifugal filters ultracel 30 k, by Merck Group (1 mentions) Hplc grade water, by Thermo Fisher Scientific (1 mentions) Methanol, by Thermo Fisher Scientific (1 mentions) Proteinase k, by Qiagen (1 mentions) 15n5 dg, by Cambridge Isotopes (1 mentions)

Spelling variants of this product

[15N5]-8-Oxo-7,8-dihydro-2′-deoxyguanosine ([15N5]-8-oxodG) (2 citations)

3 protocols using 15n5 8 oxodg

Oxidative DNA Damage Quantification Protocol

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Toluene and acetonitrile (LC-MS grade) were purchased from Merck (Darmstadt, Germany), and formic acid was from Sigma-Aldrich (St Louis, MO, USA). β-Glucuronidase (Escherichia coli) was obtained from Roche Diagnostics (Mannheim, Germany). Water was produced by the Milli-Q Integral 5 system, Millipore (Billerica, MA, USA). The analytical standard 8-oxodG was purchased from Sigma-Aldrich, and ¹⁵N₅-8-oxodG was from Cambridge Isotope Laboratories Inc. (Tewksbury, MA, USA). All other chemical standards and deuterium-labeled internal standards were purchased from Toronto Research Chemicals (Toronto, ON, Canada). NaCl (>99.5%) was purchased from Sigma-Aldrich and ultrapure water was from VWR Chemicals (Darmstadt, Germany).

Krais A.M., Essig J.Y., Gren L., Vogs C., Assarsson E., Dierschke K., Nielsen J., Strandberg B., Pagels J., Broberg K., Lindh C.H., Gudmundsson A, & Wierzbicka A. (2021). Biomarkers after Controlled Inhalation Exposure to Exhaust from Hydrogenated Vegetable Oil (HVO). International Journal of Environmental Research and Public Health, 18(12), 6492.

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Oxidative Stress Biomarkers Quantification

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8-oxodG was purchased from Sigma-Aldrich (St. Louis, MO, USA); ¹⁵N₅-8-oxodG was from Cambridge Isotope Laboratories, Inc. (Andover, MA, USA), and 8-iso-PGF_2α and 8-iso-PGF_2α-d₄ were from Cayman Chemicals Co. (Ann Arbor, MI, USA). Amicon® Ultra centrifugal filters (Ultracel®-30 K) were purchased from Merck Millipore Ltd. (Cork, Ireland), and Sep-Pak® C18 solid-phase extraction (SPE) cartridges (3 cc, 500 mg) were purchased from Waters (Milford, MA, USA). Purified water was obtained from Milli-Q® Integral system (Merck Millipore Ltd., Cork, Ireland). All other chromatography-grade chemicals were obtained from Sigma-Aldrich.

Turnu L., Di Minno A., Porro B., Squellerio I., Bonomi A., Manega C.M., Werba J.P., Parolari A., Tremoli E, & Cavalca V. (2017). Assessing Free-Radical-Mediated DNA Damage during Cardiac Surgery: 8-Oxo-7,8-dihydro-2′-deoxyguanosine as a Putative Biomarker. Oxidative Medicine and Cellular Longevity, 2017, 9715898.

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Analytical Standards for Oxidative DNA Adducts

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Nucleic acid purification grade lysis buffer, protein precipitation solution, and proteinase K were purchased from Gentra Systems (Minneapolis, MN). HPLC grade water and methanol were from Thermo Fisher Scientific Company (Raleigh, NC). ¹⁵N₅-8-oxo-dG, ¹⁵N₅-dG and ¹³C₁₀-dG were purchased from Cambridge Isotope Laboratories (Andover, MA, USA). Other chemical reagents were from Sigma-Aldrich Chemical Company (St. Louis, MO). ¹⁵N₅-1,N⁶-εdA standard was synthesized as described by Ham et al.^{62 (link)} 1,N²-εdG and ¹³C₁₀-1,N²-εdG were synthesized as reported by Kusmierek et al.^{63 (link)} MDA-modified ¹⁵N₅ and ¹⁴N₅ DNA were made by the method in Jeong's study.^{64 (link)} AcrdG, CrdG, and HNEdG standards and their ¹⁵N₅-labeled internal standards were synthesized according to previously published methods.^{65 (link)–67 (link)}

Mutlu E., Gao L., Collins L.B., Walker N.J., Hartwell H.J., Olson J.R., Sun W., Gold A., Ball L.M, & Swenberg J.A. (2016). Polychlorinated Biphenyls Induce Oxidative DNA Adducts in Female Sprague–Dawley Rats. Chemical research in toxicology, 29(8), 1335-1344.

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