F nucleatum

Manufactured by Merck Group

Sourced in United States

F.nucleatum is a laboratory equipment product that is designed for the detection and identification of the anaerobic bacterium Fusobacterium nucleatum. It provides a reliable and efficient method for the detection of this microorganism, which is commonly found in the human oral cavity and gastrointestinal tract.

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Lab products found in correlation

S mitis, by American Type Culture Collection (1 mentions) Hemin, by Merck Group (1 mentions) S oralis, by American Type Culture Collection (1 mentions) F nucleatum, by American Type Culture Collection (1 mentions) Bhi broth, by Acumedia (1 mentions) Bhi broth, by Merck Group (1 mentions) Anaerobic chamber, by Don Whitley Scientific (1 mentions) P gingivalis, by American Type Culture Collection (1 mentions) T forsythia, by American Type Culture Collection (1 mentions) N acetylmuramic acid, by Merck Group (1 mentions) P gingivalis, by Merck Group (1 mentions) T denticola, by American Type Culture Collection (1 mentions) As 580, by Anaerobe Systems (1 mentions) Mtge anaerobic enrichment broth, by Anaerobe Systems (1 mentions)

2 protocols using f nucleatum

Culturing Oral Bacterial Strains

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S. mitis (ATCC 49456), S. oralis (ATCC 35037),
and F. nucleatum (ATCC 25586) strains were purchased from the
American Type Culture Collection. S. mitis and S.
oralis were cultured in brain heart infusion (BHI) broth (Acumedia)
in a humidified incubator with 5% CO₂ at 37°C. F.
nucleatum was cultured in BHI broth supplemented with 0.5% yeast
extract, 5 µg/mL hemin (Sigma-Aldrich), and 1 µg/mL vitamin K, and incubated at
37°C in an anaerobic chamber (Don Whitley Scientific).

Makkar H., Atkuru S., Tang Y.L., Sethi T., Lim C.T., Tan K.S, & Sriram G. (2022). Differential immune responses of 3D gingival and periodontal connective tissue equivalents to microbial colonization. Journal of Tissue Engineering, 13, 20417314221111650.

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Anaerobic Cultivation of Oral Bacteria

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Bacterial strains and plasmids used in this study are shown in Table 1. A. actinomycetemcomitans (ATCC 43718), F. nucleatum subsp. nucleatum (ATCC 25586), P. gingivalis (ATCC 33277), T. forsythia (ATCC 43037), and T. denticola (ATCC 33521) were obtained from American Type Culture Collection (Manassas, VA, USA). Bacterial cultures were grown under anaerobic growth conditions at 37 °C using an anaerobic growth chamber AS‐580 (Anaerobe Systems, Morgan Hill, CA, USA) supplied with anaerobic gas (90% N₂, 5% H₂, and 5% CO₂). F. nucleatum and P. gingivalis were cultured in brain heart infusion, T. forsythia was cultured using brain heart infusion supplemented with 2.5 mM N‐acetylmuramic acid (Sigma, St. Louis, MO, USA). A. actinomycetemcomitans and T. denticola were cultured in MTGE‐anaerobic enrichment broth (Anaerobe Systems).

Coffey J., Choudhry M., Shlossman M., Makin I.R, & Singh V.K. (2016). Multiplex real‐time PCR detection and relative quantification of periodontal pathogens. Clinical and Experimental Dental Research, 2(3), 185-192.

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