Axio imager z1 fluorescent

Manufactured by Zeiss

Sourced in Germany

The Axio Imager Z1 is a fluorescent microscope system designed for advanced imaging applications. It features a motorized focus drive and a range of fluorescence illumination options to enable high-quality visualization and documentation of samples.

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Lab products found in correlation

D5000, by Nikon (1 mentions) Axiovision 4, by Zeiss (1 mentions) Axiovision rel 4, by Zeiss (1 mentions) Superfrost plus, by Thermo Fisher Scientific (1 mentions) Axiocam mrm camera, by Zeiss (1 mentions) Zen 2, by Zeiss (1 mentions) Vectashield, by Vector Laboratories (1 mentions)

Close spelling variants of this product

Axio Imager (671 citations) Axio Imager Z1 (662 citations) Imager Z1 (138 citations) Axio Imager Z1 fluorescent microscope (37 citations)

4 protocols using axio imager z1 fluorescent

Fluorescence Microscopy Imaging Protocol

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The photographs were made with a Nikon Eclipse E600 fluorescent microscope, using the B-2A filter block (EX:450; DM > 505; BA:520). The micrographs were made with a SPOT RT-SE™ Digital Camera (Diagnostic Instruments) or with a Nikon D5000 camera. Frozen tissue sections were analyzed by Zeiss Axio Imager Z1 fluorescent microscope and Zeiss AxioVision 4.6.3 software. The contrast of the micrographs was adjusted with the Adobe Photoshop software. The same corrections were applied for the experimental samples and their appropriate controls. The YFP fluorescence was visualized with a pseudo-green colour.

Jósvay K., Winter Z., Katona R.L., Pecze L., Marton A., Buhala A., Szakonyi G., Oláh Z, & Vizler C. (2014). Besides neuro-imaging, the Thy1-YFP mouse could serve for visualizing experimental tumours, inflammation and wound-healing. Scientific Reports, 4, 6776.

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Visualizing TMV Trafficking in MDA-MB-231 Cells

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Intracellular trafficking was monitored in MDA-MB-231 cells; 25,000 cells were seeded overnight on glass coverslips and incubated for 8 h with 1 × 10⁶ TMV particles per cell. Following incubation, cells were washed, fixed, and stained. Cell membrane was stained using wheat germ agglutinin conjugated to AlexaFluor 555. TMV was stained using a rabbit anti-TMV antibody primary and a goat antirabbit secondary conjugated to AlexaFluor 647. Endolysosomes were stained using a mouse antihuman Lamp-1 antibody primary and a goat antimouse secondary conjugated to AlexaFluor 488. Slides were imaged using Zeiss Axio Imager Z1 fluorescent inverted high-resolution microscope with motorized stage.

Czapar A.E., Zheng Y.R., Riddell I.A., Shukla S., Awuah S.G., Lippard S.J, & Steinmetz N.F. (2016). Tobacco Mosaic Virus Delivery of Phenanthriplatin for Cancer therapy. ACS nano, 10(4), 4119-4126.

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Fluorescent Microscopy of GFP-Expressing Bacteria

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The cultures containing GFP-producing bacteria were harvested, and the pellets were suspended in 1 × PBS. Bacterial suspensions of 20 μl or slices of cross-sectioned tissue were mounted on microscope slides (Superfrost Plus, Thermo Scientific). Live cells were observed under an Axio Imager Z1 fluorescent microscope equipped with an AxioCam MRm camera (Zeiss, Jena, Germany). The GFP signal was detected using the filter set 10 (Cy2/GFP). All images were captured with a 63X magnification oil objective with an aperture of 1.4. The images were analyzed using the Axio Vision Rel 4.8 software (Zeiss, Jena, Germany). ImageJ, Fiji (Schindelin et al., 2012 (link)), an open-source software, was used to process all fluorescent images.

Teh B.S., Apel J., Shao Y, & Boland W. (2016). Colonization of the Intestinal Tract of the Polyphagous Pest Spodoptera littoralis with the GFP-Tagged Indigenous Gut Bacterium Enterococcus mundtii. Frontiers in Microbiology, 7, 928.

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Immunofluorescence Assay for T. gondii

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Confluent HFF cells were grown on glass coverslips and infected with T. gondii. After 18–40 hours, the coverslips were fixed with 3.7% formaldehyde in PBS and processed for immunofluorescence as described [70 (link)]. Primary antibodies were detected by species-specific secondary antibodies conjugated to Alexa Fluor 594/488/405 (ThermoFisher). Coverslips were mounted in Vectashield (Vector Labs), viewed with an Axio Imager.Z1 fluorescent microscope and processed with ZEN 2.3 software (Zeiss).

Pasquarelli R.R., Back P.S., Sha J., Wohlschlegel J.A, & Bradley P.J. (2023). Identification of IMC43, a novel IMC protein that collaborates with IMC32 to form an essential daughter bud assembly complex in Toxoplasma gondii. PLOS Pathogens, 19(10), e1011707.

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