DEHP was purchased from Sigma-Aldrich (St. Louis, MO, USA), Hericium erinaceus was from Mycology Research Laboratories Ltd. (Luton, United Kingdom), 3-4,5-Dimethylthiazol-2-yl, 2,5-diphenyltetrazolium bromide (MTT), Cell culture medium Dulbecco’s modified Eagle medium (DMEM), horse serum, fetal bovine serum (FBS), phosphate buffer saline (PBS), trypsin-EDTA, penicillin and streptomycin mixture and l-glutamine (200 mM) were from GIBCO-BCL (UK). 2,7-Dichlorofluoresce diacetate (DCFH-DA) was supplied by Molecular Probes (Cergy Pontoise, France). All other chemicals used were of analytical grade.
2 7 dichlorofluoresce diacetate dcfh da
Manufactured by Thermo Fisher Scientific
Sourced in France
2,7-Dichlorofluoresce diacetate (DCFH-DA) is a fluorogenic probe used in cell-based assays. It is a non-fluorescent compound that becomes fluorescent upon oxidation by reactive oxygen species or free radicals within the cell. The degree of fluorescence generated is proportional to the level of oxidative stress in the sample.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using 2 7 dichlorofluoresce diacetate dcfh da
1
Cytotoxicity Evaluation of Hericium erinaceus
2
Cytotoxicity Evaluation of Triflumuron and Fennel
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Triflumuron (TFM) and Fennel Essential Oil (FEO) were purchased from Sigma-Aldrich (St. Louis, MO, USA). 3-4,5-Dimethylthiazol-2-yl, 2,5-diphenyltetrazolium bromide (MTT), cell culture medium (MEM), foetal calf serum (FCS), phosphate buffer saline (PBS), trypsin-EDTA, penicillin and streptomycin mixture and L-glutamine (200 mM) were from GIBCO-BCL (UK). 2, 7-Dichlorofluoresce diacetate (DCFH-DA) was supplied by Molecular Probes (Cergy Pontoise, France). Low melting point agarose (LMA) and normal melting point agarose (NMA) were purchased from Sigma (St. Louis, MO, USA). Mouse anti-Hsp 70 (SP-810) was from stressgen (Canada), mouse monoclonal antibax and anti-bcl2 were from Invitrogen (Carlsbad, California, USA) and mouse anti -GAPDH (ab983) was from ABCAM. All other chemicals used were of analytical grade. All other chemicals used were of analytical grade.
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