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2 protocols using peg 8 kda

1

Synthetic Clay Mineral Characterization

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Three types of clay were supplied from the Clay Mineral Society Source Clays Repository: kaolinite, Warren County, Georgia, USA (KGa-2); Na-rich montmorillonite, Crook County, Wyoming, USA (SWy-2); and Ca-rich montmorillonite, Gonzales County, Texas, USA (STx-1b). NX-illite was obtained from Arginotec, NX Nanopowder, B + M Notenkämper, Munich, Germany. NX-Illite is composed of illite, illite-smectite mixed layer, feldspar, kaolinite, quartz and carbonate52 (link).
PEG 8 kDa, dextran 10 kDa, 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES), HEPES sodium salt, sodium bicarbonate, o-phenylenediamine tablets, hydrogen peroxide and 2,3-diaminophenazine were purchased from Sigma-Aldrich (St. Louis, MO). Labeled polymer mPEG-NH2 MW 5000 was purchased from Shearwater Polymers. Alexa Fluor 647-dextran 10 kDa, Alexa Fluor 488 labeling kits and 20 mm diameter by 0.5 mm deep press-to-seal silicone isolators were obtained from Life Technologies (Carlsbad, CA). Water was deionized to a resistance of 18.2 MΩ with Barnstead NANOpure Diamond water purification system (Van Nuys, CA) and used for all experiments.
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2

Analytical Characterization of PEG-Dextran Hydrogels

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PEG
8 kDa, dextran 10 kDa from Leuconostoc mesenteroides, PEG 400, d-(+)-glucose,
30% hydrogen peroxide solution, OPD, 3,3′,5,5′-tetramethlybenzidene,
sodium phosphate dibasic dihydrate, sodium phosphate monobasic dihydrate,
deuterium oxide, DMSO-d6, and Amicon 0.5
mL filters (MWCO 3000) were purchased from Sigma-Aldrich (St. Louis,
MO). HRP EIA grade was purchased from Life Technologies (Carlsbad,
CA). DMSO was purchased from Alfa Aesar, and ethylenediaminetetraacetic
acid (EDTA) was purchased from IBI Scientific (Peosta, IA). Deionized
water with a resistivity of 18.2 MΩ·cm from a Barnstead
NANOpure Diamond water purification system (Van Nuys, CA) was used
for all experiments. Buffers were filtered using a 0.45 μm pre
size Nalgene filter units. All reagents were used as received without
further purification. Viscosity measurements were made using an Ostwald
viscometer.
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