We used an Annexin V-APC/7-AAD Apoptosis Detection Kit I (BD Pharmingen™, New Jersey, USA) for the apoptosis assay. NORAD knockdown and NC cells were harvested and resuspended (5 × 105 cells per sample) before 5 μl of Annexin V-APC and 7-AAD were added to the suspensions and incubated for 15 min at room temperature in the dark. Flow cytometry was used to evaluate the luciferase intensity of Annexin V-APC and 7-AAD. A Cell Cycle Staining Kit (BD Pharmingen™, New Jersey, USA) was used to evaluate the cell cycle distribution. Cells were fixed with 70% ethanol for 2 h at 4 °C. Target cells were treated with the DNA staining solution and permeabilization solution in the dark for 15 min at room temperature. Flow cytometry was used to evaluate the luciferase intensity at 24 h after treatment.
Annexin 5 apc 7 aad apoptosis detection kit 1
Manufactured by BD
Sourced in United States
The Annexin V-APC/7-AAD Apoptosis Detection Kit is a laboratory tool used for the detection and analysis of apoptosis, a process of programmed cell death. The kit utilizes Annexin V, a protein that binds to phosphatidylserine, and 7-AAD, a DNA-binding dye, to identify cells undergoing early and late stages of apoptosis.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using annexin 5 apc 7 aad apoptosis detection kit 1
1
Apoptosis and Cell Cycle Analysis
2
Apoptosis and Cell Cycle Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
We used an Annexin V-APC 7-AAD Apoptosis Detection Kit I (BD Pharmingen TM, New Jersey, USA) for the apoptosis assay. NORAD knockdown and NC cells were harvested and resuspended (5×10 5 cells per sample) before 5 μl of Annexin V-APC and 7-AAD were added to the suspensions and incubated for 15 minutes at room temperature in the dark. Flow cytometry was used to evaluate the luciferase intensity of Annexin V-APC and 7-AAD. A Cell Cycle Staining Kit (BD Pharmingen TM, New Jersey, USA) was used for evaluating the cell cycle distribution. Cells were xed with 70% ethanol for 2 hours at 4°C. Target cells were treated with DNA staining solution and permeabilization solution in darkness for 15 minutes at room temperature. Flow cytometry was used to evaluate the luciferase intensity at 24 hours after treatment.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!