After culturing cells for 72 h at 37°C containing 5% CO2 in a humidified incubator, the culture medium was collected separately and centrifuged at 1,000 rpm for 5 min to remove debris. The supernatants were then frozen at −80°C for further assessment by ELISA. ELISA was performed using the human CXCL12/SDF-1 kit (R&D Systems, Minneapolis, MN, USA) according to the manufacturer’s instructions.
Human cxcl12 sdf 1 kit
Manufactured by R&D Systems
Sourced in United States
The Human CXCL12/SDF-1 kit is a reagent kit designed for the quantitative determination of human CXCL12/SDF-1 levels in cell culture supernatants, serum, and plasma. It utilizes the sandwich enzyme immunoassay technique.
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Lab products found in correlation
2 protocols using human cxcl12 sdf 1 kit
1
CXCL12/SDF-1 Quantification by ELISA
2
CXCL12/SDF-1 Secretion in BMSC, MG-63, U2OS
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After culturing BMSCs, MG-63, and U2OS cells for 72 h, the medium was collected separately and centrifuged at 1000 r/min for 5 min. The supernatants were then stored at -80°C for further enzyme-linked immunosorbent assay (ELISA) test. ELISA was performed using the human CXCL12/SDF-1 kit (R&D Systems) according to the manufacturer's instructions.
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