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Gs junior sequencing

Manufactured by Roche
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The GS Junior sequencing system is a bench-top next-generation sequencing platform developed by Roche. It is designed to perform small-scale, targeted DNA sequencing. The GS Junior system utilizes pyrosequencing technology to generate sequence data, providing a flexible and efficient solution for various applications in life science research.

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Lab products found in correlation

Quant it picogreen dsdna reagent, by Thermo Fisher Scientific (2 mentions) Gs junior sequencer, by Roche (2 mentions) Gs junior titanium empcr kit lib a kit, by Roche (2 mentions) Agencourt ampure xp, by Beckman Coulter (2 mentions)

Close spelling variants of this product

GS Junior Titanium Sequencing Kit Roche GS Junior sequencing platform GS Junior Sequencing system 454 GS junior sequencing system GS Junior

2 protocols using gs junior sequencing

1

Amplicon Sequencing of Genomic DNA

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Amplicon sequencing was performed using previously published methods (48 (link)). We performed locus-specific amplification of genomic DNA followed by GS Junior sequencing (Roche). We designed fusion primers containing genome-specific sequences along with distinct multiplex identifier sequences (used to differentiate samples being run together on the same plate) and sequencing adapters, to generate amplicons ranging in size from 290 to 310 bp, using Primer3Plus software. Primer sequences are provided in Supplemental Table 4. Small DNA fragments were removed with Agencourt AMPure XP (Beckman Coulter) according to the manufacturer’s protocol. All amplicons were quantified with the Quant-iT PicoGreen dsDNA reagent (Life Technologies), pooled at equimolar ratios, amplified by emulsion PCR using the GS Junior Titanium emPCR kit (Lib-A kit, Roche Applied Science), and pyrosequenced in the sense and antisense strands on a GS Junior sequencer following the manufacturers’ instructions. Data were analyzed using GS Amplicon Variant Analyzer version 3.0 software.
Mirzaa G., Timms A.E., Conti V., Boyle E.A., Girisha K.M., Martin B., Kircher M., Olds C., Juusola J., Collins S., Park K., Carter M., Glass I., Krägeloh-Mann I., Chitayat D., Parikh A.S., Bradshaw R., Torti E., Braddock S., Burke L., Ghedia S., Stephan M., Stewart F., Prasad C., Napier M., Saitta S., Straussberg R., Gabbett M., O’Connor B.C., Keegan C.E., Yin L.J., Lai A.H., Martin N., McKinnon M., Addor M.C., Boccuto L., Schwartz C.E., Lanoel A., Conway R.L., Devriendt K., Tatton-Brown K., Pierpont M.E., Painter M., Worgan L., Reggin J., Hennekam R., Tsuchiya K., Pritchard C.C., Aracena M., Gripp K.W., Cordisco M., Van Esch H., Garavelli L., Curry C., Goriely A., Kayserilli H., Shendure J., Graham J J.r., Guerrini R, & Dobyns W.B. (2016). PIK3CA-associated developmental disorders exhibit distinct classes of mutations with variable expression and tissue distribution. JCI Insight, 1(9), e87623.
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2

Amplicon Sequencing for Genomic Analysis

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Amplicon sequencing was performed using previously published methods (48 (link)). We performed locus-specific amplification of genomic DNA followed by GS Junior sequencing (Roche). We designed fusion primers containing genome-specific sequences along with distinct multiplex identifier sequences (used to differentiate samples being run together on the same plate) and sequencing adapters, to generate amplicons ranging in size from 290 to 310 bp, using Primer3Plus software. Primer sequences are provided in Supplemental Table 4. Small DNA fragments were removed with Agencourt AMPure XP (Beckman Coulter) according to the manufacturer’s protocol. All amplicons were quantified with the Quant-iT PicoGreen dsDNA reagent (Life Technologies), pooled at equimolar ratios, amplified by emulsion PCR using the GS Junior Titanium emPCR kit (Lib-A kit, Roche Applied Science), and pyrosequenced in the sense and antisense strands on a GS Junior sequencer following the manufacturers’ instructions. Data were analyzed using GS Amplicon Variant Analyzer version 3.0 software.
Mirzaa G., Timms A.E., Conti V., Boyle E.A., Girisha K.M., Martin B., Kircher M., Olds C., Juusola J., Collins S., Park K., Carter M., Glass I., Krägeloh-Mann I., Chitayat D., Parikh A.S., Bradshaw R., Torti E., Braddock S., Burke L., Ghedia S., Stephan M., Stewart F., Prasad C., Napier M., Saitta S., Straussberg R., Gabbett M., O’Connor B.C., Keegan C.E., Yin L.J., Lai A.H., Martin N., McKinnon M., Addor M.C., Boccuto L., Schwartz C.E., Lanoel A., Conway R.L., Devriendt K., Tatton-Brown K., Pierpont M.E., Painter M., Worgan L., Reggin J., Hennekam R., Tsuchiya K., Pritchard C.C., Aracena M., Gripp K.W., Cordisco M., Van Esch H., Garavelli L., Curry C., Goriely A., Kayserilli H., Shendure J., Graham J J.r., Guerrini R, & Dobyns W.B. (2016). PIK3CA-associated developmental disorders exhibit distinct classes of mutations with variable expression and tissue distribution. JCI Insight, 1(9), e87623.
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