Female C57BL/6J (B6) mice were purchased from Jackson Laboratory (Bar Harbor, ME) at 7 to 8 weeks of age and housed in a biosafety level 2+ (BSL2+) facility at the University of Florida (Gainesville, FL) in accordance with all federal guidelines and as approved by the University of Florida Institutional Animal Care and Use Committee. For i.n. inoculations, B6 mice (Jackson Laboratory) were anesthetized with isoflurane and then inoculated with 104 PFU of virus in 30 μl serum-free DMEM. BALB.IFN-γ−/− mice (Jackson Laboratories) were inoculated with 4 × 105 PFU i.n. For i.p. inoculations, B6 mice were injected with 103 PFU virus in 500 μl serum-free DMEM. Unless otherwise noted, groups of three mice were used per time point, per experiment, for all studies. For blood harvests, blood was collected by heart puncture and placed into BD Microtainers with lithium heparin (catalog number 365965; BD). Buffy coats were separated using lympholyte-M cell separation medium (cI5030; Cedarlane).
Microtainers with lithium heparin
Manufactured by BD
BD Microtainers with lithium heparin are blood collection tubes used to collect and store venous blood samples. The tubes contain lithium heparin as an anticoagulant to prevent the blood from clotting. The BD Microtainers are designed to be small and easy to handle, suitable for various laboratory testing procedures.
Automatically generated - may contain errors
2 protocols using microtainers with lithium heparin
1
Intranasal Inoculation of Mice with Virus
2
In vivo Infection of C57BL/6J and BALB.IFN-γ-/- Mice
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Female C57BL/6J (B6) mice were purchased from Jackson Laboratory (Bar Harbor, ME) at 7 to 8 weeks of age and housed in a biosafety level 2+ (BSL2+) facility at the University of Florida (Gainesville, FL) in accordance with all federal guidelines and as approved by the University of Florida Institutional Animal Care and Use Committee. For i.n. inoculations, B6 mice (Jackson Laboratory) were anesthetized with isoflurane and then inoculated with 104 PFU of virus in 30 µl serum-free DMEM. BALB.IFN-γ−/− mice (Jackson Laboratories) were inoculated with 4 × 105 PFU i.n. For i.p. inoculations, B6 mice were injected with 103 PFU virus in 500 µl serum-free DMEM. Unless otherwise noted, groups of three mice were used per time point, per experiment, for all studies. For blood harvests, blood was collected by heart puncture and placed into BD Microtainers with lithium heparin (catalog number 365965; BD). Buffy coats were separated using lympholyte-M cell separation medium (cI5030; Cedarlane).
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