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Avatar 360 thermo nicolet spectrometer

Manufactured by Thermo Fisher Scientific

The Avatar 360 Thermo Nicolet Spectrometer is a laboratory instrument designed for spectroscopic analysis. It utilizes infrared radiation to identify and quantify the chemical composition of various samples.

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2 protocols using avatar 360 thermo nicolet spectrometer

1

Functional Group Analysis of PCL Scaffolds

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Functional groups in uncoated PCL, HS-coated PCL and collagen-coated PCL were compared via attenuated total reflection-Fourier transform infrared spectroscopy (ATR-FTIR) using an Avatar 360 Thermo Nicolet Spectrometer (Thermo Scientific, Waltham, MA). Scaffolds were placed in contact with the diamond ATR crystal to obtain the absorbance spectra between 1,500 cm−1 and 4,000 cm−1 wavenumber. Results were converted to percent transmittance, and the functional group region between 1,500 cm−1 and 4,000 cm−1 was analyzed.
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2

FTIR Analysis of TCP Particle Surfaces

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FTIR spectroscopy was performed to analyze the TCP particle surface. Each of the samples (HA-TCP, Chitosan, and Chi/HA-TCP particles) was placed onto the diamond ATR window of an Avatar 360 Thermo Nicolet spectrometer and scanned over the range of 500-4000 cm−1 with a resolution of 4 cm−1 in transmission mode. The baselines of the spectra were corrected and normalized using OMNIC program (ThermoScientific software for FTIR data collection and analysis).
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