Flash scanner

Manufactured by PerkinElmer

The Flash Scanner is a high-speed imaging system designed for rapid and precise sample scanning. It captures images at a fast rate, enabling efficient data collection and analysis.

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Lab products found in correlation

Panoramic viewer, by 3DHISTECH (2 mentions) Ripa buffer, by Santa Cruz Biotechnology (1 mentions) Alexa fluor 488, by Thermo Fisher Scientific (1 mentions) Prolong diamond antifade mountant, by Thermo Fisher Scientific (1 mentions) β pix, by Merck Group (1 mentions) Cdc42, by Cell Signaling Technology (1 mentions)

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Pannoramic Flash 250 scanner (3 citations)

3 protocols using flash scanner

Immunofluorescence and Western Blot Analysis of Cdc42, Rac1, and RhoA Signaling Pathways

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Primary antibodies were obtained targeting: Cdc42 (rabbit polyclonal, Cell Signaling), Rac1 (mouse monoclonal, Cytoskeleton), RhoA (mouse monoclonal, Santa Cruz), β-Pix (rabbit polyclonal, Millipore), Tuj1 (mouse monoclonal, Millipore), active Cdc42 (mouse monoclonal, New East Biosciences), Ret (pig anti-human, Abgent). The secondary antibodies used included: goat anti-mouse (Santa Cruz) and goat anti-rabbit (Santa Cruz). For western blots, cells were lysed with RIPA buffer (SantaCruz), boiled and separated by 3-8% Tris-Acetate SDS-PAGE.
For immunofluorescence microscopy, antibodies used included RET (rabbit monoclonal, Cell Signaling), p-Ret (phospho-Y1062, rabbit polyclonal, Abcam), β-Pix (SH3 domain, rabbit polyclonal, Millipore Sigma), and Cdc42 (rabbit polyclonal, Cell Signaling). Cells were fixed in 4% formaldehyde and permeabilized in 0.5% Triton X-100 (vol/vol), or fixed in 3.7% paraformaldehyde and blocked with 3% horse serum in 0.1% Triton in PBS for 60 minutes. The secondary antibody was Alexa Fluor 488 (goat anti-rabbit, Thermo Fisher Scientific). Slides were mounted with ProLong Diamond Antifade Mountant (Invitrogen, Thermo Fisher Scientific) and were scanned with a Flash Scanner (Perkin Elmer) and a Panoramic Viewer (3DHistech, Thermo Fisher Scientific) was used to capture images.

Chernichenko N., Omelchenko T., Deborde S., Bakst R., He S., Chen C.H., Gusain L., Vakiani E., Katabi N., Hall A, & Wong R.J. (2020). Cdc42 mediates cancer cell chemotaxis in perineural invasion. Molecular cancer research : MCR, 18(6), 913-925.

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Immunofluorescence of Murine and Human Tissues

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Murine frozen sections were fixed using 4% paraformaldehyde. Sections from human pancreatic specimens were obtained from paraffin blocks that were prepared using a standard protocol. Sections were permeabilized and blocked in 3% horse serum, 0.1% Triton X-100/ PBS for 1h. Primary antibodies (anti P-c-Jun 1:200, anti GFAP 1:1000, anti-cytokeratin 1:200) diluted in 0.1% horse serum, 0.1% Triton X-100/PBS were incubated O/N at 0034°C. Sections were washed with PBS and detection was performed using an appropriate fluorescent secondary antibody (Alexa Fluor 488, 568, or 647, Invitrogen). Samples were mounted in DAPI containing antifade mounting medium. Slides were scanned using Flash Scanner (Perkin Elmer). Immunofluorescent sections were analyzed using Panoramic Viewer (3DHISTECH).

Deborde S., Gusain L., Powers A., Marcadis A., Yu Y., Chen C.H., Frants A., Kao E., Tang L.H., Vakiani E., Amisaki M., Balachandran V.P., Calo A., Omelchenko T., Jessen K.R., Reva B, & Wong R.J. (2022). Reprogrammed Schwann Cells Organize into Dynamic Tracks that Promote Pancreatic Cancer Invasion. Cancer Discovery, 12(10), 2454-2473.

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Immunofluorescent Staining of Murine and Human Tissues

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Murine frozen sections were fixed using 4% paraformaldehyde. Sections from human pancreatic specimens were obtained from paraffin blocks that were prepared using a standard protocol. Sections were permeabilized and blocked in 3% horse serum and 0.1% Triton X-100/PBS for 1 hour. Primary antibodies (anti–P-c-Jun 1:200, anti-GFAP 1:1,000, anti-cytokeratin 1:200) diluted in 0.1% horse serum and 0.1% Triton X-100/PBS were incubated overnight at 4°C. Sections were washed with PBS, and detection was performed using an appropriate fluorescent secondary antibody (Alexa Fluor 488, 568, or 647; Invitrogen). Samples were mounted in DAPI containing antifade mounting medium. Slides were scanned using Flash Scanner (PerkinElmer). Immunofluorescent sections were analyzed using Panoramic Viewer (3DHISTECH).

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