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Ec plan neofluar 40 na 0.75 objective

Manufactured by Zeiss

The EC Plan-NEOFLUAR 40 × NA 0.75 objective is a high-numerical aperture objective lens designed for use in advanced optical microscopy applications. It provides a magnification of 40× and a numerical aperture of 0.75, which allows for high-resolution imaging and light collection.

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3 protocols using ec plan neofluar 40 na 0.75 objective

1

Quantifying PDGF-Induced Vimentin Dynamics

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NIH3T3 cells stably expressing mCherry-vimentin were treated with 50 ng/mL PDGF in the presence or absence 200 nM dasatinib. The cells were incubated in a micro-cultivation system with temperature and CO2 control devices (Carl Zeiss). The cells were monitored on an inverted microscope (Axio Observer; Carl Zeiss) using a EC Plan-NEOFLUAR 40 × NA 0.75 objective. Images were captured every 15 sec for 1–3 h using a digital camera (ORCA-Flash4.0 V2; Hamamatsu) and were processed by ZEISS ZEN2 image software.
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2

Time-Lapse Imaging of TGFβ-Treated A549 Cells

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A549 cells were transiently transfected with the GFP-H2B plasmid and cultured in the presence or absence of TGFβ for 12 h. Cells were then incubated in a micro-cultivation system with temperature and CO2control devices (Carl Zeiss). The cells were monitored on an inverted microscope (Axio Observer; Carl Zeiss) using an EC Plan-NEOFLUAR 40 × NA 0.75 objective. Images were captured every 10 min for 12 h using a digital camera (ORCA-Flash4.0 V2; Hamamatsu) and were processed by the ZEISS ZEN2 image software.
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3

Time-lapse Imaging of HeLa Cell Dynamics

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HeLa or LMNA−/− HeLa cells expressing mCherry vector alone or mCherry-lamin A were incubated in a micro-cultivation system with temperature and CO2 control devices (Carl Zeiss). The cells were monitored on an inverted microscope (Axio Observer; Carl Zeiss) using an EC Plan-NEOFLUAR 40 × NA 0.75 objective. Images were captured every 10 min for 24 h using a digital camera (ORCA-Flash4.0 V2; Hamamatsu) and were processed by ZEISS ZEN2 image software.
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