Agilent 1290 infinity 2 series hplc

Proteomics was performed on 50 grown oocytes/group recovered by slicing ovaries in PBS. ZP was solubilized by incubation at 75°C for 45 min (Izquierdo-Rico et al., 2009 (link)) and analyses were carried out on one technical replicate with a HPLC/MS system consisting of an Agilent 1290 Infinity II Series HPLC (Agilent Technologies, Santa Clara, CA, USA) equipped with an Automated Multisampler module and a High Speed Binary Pump, and connected to an Agilent 6550 Q-TOF Mass Spectrometer (Agilent Technologies, Santa Clara, CA, USA) using an Agilent Jet Stream Dual electrospray (AJS-Dual ESI) interface. Experimental parameters for HPLC and Q-TOF were set in MassHunter Workstation Data Acquisition software (Agilent Technologies, Rev. B.08.00). Data processing and analysis was performed on Spectrum Mill MS Proteomics Workbench (Rev B.06.00.201, Agilent Technologies, Santa Clara, CA, USA).

UHPLC-HRMS analyses were performed using an Agilent 1290 Infinity II Series HPLC (Agilent Technologies, Santa Clara, CA, USA) with a high-speed binary pump (thereby comprising the UHPLC system) coupled to an Agilent 6550 QTOF Mass Spectrometer using an Agilent jet stream dual electrospray (AJS-Dual ESI) source. MassHunter workstation software from Agilent Technologies (Version B.08.00, Santa Clara, CA, USA) was used for data acquisition and MS-DIAL (Version 4.80, RIKEN, Yokohama, Japan) was used for data interpretation.
For sample processing, an Xcelvap air-drying system from Horizon Technology (Salem, MA, USA) and an orbital shaker IKA-KS-130-Basic (Staufen, Germany) were used. The permanent magnets employed were blocks consisted of Nd-Fe-B with a strength, dimensions and weight of 33 kg, 50 × 15 × 15 mm and 86 g, respectively. Such magnets were purchased from Supermagnete (Gottmadingen, Germany).
Image data were acquired by field emission scanning electron microscopy (FESEM) with ApreoS Thermo FESEM (ThermoFisher Scientific, MA, USA) and energy-dispersive X-ray spectroscopy (EDS) analyses were conducted using EDAX-Ametek (EDAX, AMETEK Materials Analysis Division, Mahwah, NJ, USA).
For data treatment, Sigmaplot 13.1 (Systat, Software Inc., San Jose, CA, USA) was used as statistic software.

Blood samples were collected from the hearth animals just before euthanasia. The plasma was obtained by centrifugal separation and frozen at −20 °C until analysis for determination of taurine by chromatography. This was completed with an HPLC/MS system [4 (link),6 (link)] consisting of an Agilent 1290 Infinity II Series HPLC (Agilent Technologies, Santa Clara, CA, USA) equipped with an Automated Multisampler module and a High Speed BinaryPump and connected to an Agilent 6550 Q-TOF Mass Spectrometer (Agilent Technologies, Santa Clara, CA, USA) using an Agilent Jet Stream Dual electrospray (AJS-Dual ESI) interface. Experimental parameters for HPLC and Q-TOF were set in MassHunter Workstation Data Acquisition software (Agilent Technologies, Santa Clara, CA, USA, Rev. B.08.00).