4 ohe2

4-OHE₂ and E₂ were purchased from Sigma Chemical Co. Rabbit polyclonal HO-1 antibody was purchased from Stressgen. The primary antibody of Nrf2, Keap1, and PCNA were purchased from Santa Cruz Biotechnology. Anti-CA 15–3 was obtained from Fitzgerald. The primary antibody for COX-2 was purchased from Neomarker. ZnPP was supplied from Alexis Corporation. Matrigel basement membrane matrix was purchased from BD Bioscience. HO-1 short interfering RNA (siRNA) was purchased from Invitrogen. HO-1 plasmid was kindly provided by Prof. Jozef Dulak (Jagiellonian University, Krakow, Poland). N-Acetyl-L-cysteine (NAC), trolox, DTT, NEM and an antibody against actin were also purchased from Sigma Chemical Co. The primary antibodies of lamin B and α-tubulin and anti-rabbit horseradish peroxidase-conjugated secondary antibody were products of Zymed Laboratories Inc. The inhibitors of ERK (U0126) and PI3K (LY294002) were purchased from TOCRIS. BPM was obtained from DOJINDO. Human recombinant Keap1 protein was purchased from Abnova. Avidin agarose was a product of Pierce. The ECL chemiluminescent detection kit was obtained from Amersham Pharmacia Biotech. The human specific Nrf2 siRNA (sense 5′-AAGAGUAUGAGCUGGAAAAACTT-3′; antisense 5′-GUUUUUCCAGCUCAUAC UCUUTT-3′) and Stealth^TM RNAi negative control duplexes were provided by Invitrogen. The luciferase assay kit was purchased from Promega.