Beckman 8000

Manufactured by Beckman Coulter

Sourced in United States

The Beckman 8000 is a high-performance automated chemistry analyzer designed for clinical laboratory settings. It provides rapid and accurate processing of a wide range of clinical chemistry tests, including electrolytes, enzymes, and other analytes. The Beckman 8000 utilizes advanced spectrophotometric technology to deliver reliable results, ensuring efficient and consistent laboratory operations.

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Lab products found in correlation

Male wild type c57bl 6 mice, by Charles River Laboratories (1 mentions)

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Beckman 8000 gamma counter (9 citations) Beckman 8000 automated gamma counter (1 citations)

7 protocols using beckman 8000

Biodistribution of 64Cu-labeled Chemokines in Mice

Cited 2 times

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The specific activities of ⁶⁴Cu-vMIP-II-Comb and
⁶⁴Cu-Comb were 3.2 MBq/nmol and 3.7 MBq/nmol, respectively (25 (link)). Male WT C57BL/6 mice weighing 20-25 g
(n = 4/group) were anesthetized with inhaled isoflurane and
approximately 370 KBq of ⁶⁴Cu-vMIP-II-Comb (~ 0.12 nmol) in
100 μL of saline were injected via the tail vein. The mice were
reanesthetized before they were euthanized by cervical dislocation at each time
point (1, 4, 24, and 48 h) after injection. Organs of interest were collected,
weighed, and counted in a well γ-counter (Beckman 8000) to calculate the
percentage injected dose per gram of tissue (%ID/gram) (31 (link)).

Luehmann H.P., Detering L., Fors B.P., Pressly E.D., Woodard P.K., Randolph G.J., Gropler R.J., Hawker C.J, & Liu Y. (2016). PET/CT Imaging of Chemokine Receptors in Inflammatory Atherosclerosis Using Targeted Nanoparticles. Journal of nuclear medicine : official publication, Society of Nuclear Medicine, 57(7), 1124-1129.

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Biodistribution of 64Cu-Labeled Nanoparticles

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⁶⁴Cu-DOTA-DAPTA-comb and ⁶⁴Cu-DOTA-comb were reconstituted in 0.9% sodium chloride (APP Pharmaceuticals) for intravenous injection. Male C57BL/6 mice weighing 20–25 g (n = 4/group) were anesthetized with inhaled isoflurane, and approximately 370 kBq of labeled nanoparticles (7.2–9.0 μg/kg of body weight) or the DOTA-DAPTA peptide (0.35–0.44 μg/kg of body weight) in 100 μL of saline were injected via the tail vein. The mice were reanesthetized before they were euthanized by cervical dislocation at each time point (1, 24, and 48 h) after injection. Organs of interest were collected, weighed, and counted in a well γ counter (Beckman 8000). Standards were prepared and measured along with the samples to calculate the percentage injected dose per gram of tissue (%ID/g) (27 (link)).

Luehmann H.P., Pressly E.D., Detering L., Wang C., Pierce R., Woodard P.K., Gropler R.J., Hawker C.J, & Liu Y. (2014). PET/CT Imaging of Chemokine Receptor CCR5 in Vascular Injury Model Using Targeted Nanoparticle. Journal of nuclear medicine : official publication, Society of Nuclear Medicine, 55(4), 629-634.

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Biodistribution of 64Cu-AuNCs-ECL1i Nanoparticles

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Purified ⁶⁴Cu-AuNCs-ECL1i nanoclusters were reconstituted in 0.9% sodium chloride (APP Pharmaceuticals) for intravenous injection. Male B6 CCR2^−/− mice (Charles River Laboratories, Boston) weighing 21–25 g (n = 4/group) were anesthetized with inhaled isoflurane, and approximately 370 kBq/100 μL saline of radiolabeled nanoclusters were injected via the tail vein. The peptide alone tracer ⁶⁴Cu-DOTA-ECL1i was also prepared for biodistribution study in male wild type C57BL/6 mice (Charles River Laboratories, Boston) weighing 20 – 25 g (n = 4/group).^{35 (link)} The mice were reanesthetized before they were euthanized by cervical dislocation at each time point (1, 4, and 24 h) post injection. Organs of interest were collected, weighed, and counted in a well gamma counter (Beckman 8000). Standards were prepared and measured along with the samples to calculate the percentage injected dose per gram of tissue (%ID/gram).^{35 (link)}

Sultan D., Li W., Detering L., Heo G.S., Luehmann H.P., Kreisel D, & Liu Y. (2021). Assessment of Ultrasmall Nanocluster for Early and Accurate Detection of Atherosclerosis Using Positron Emission Tomography/Computed Tomography. Nanomedicine : nanotechnology, biology, and medicine, 36, 102416.

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64Cu-DOTA-ICP Biodistribution in Mice

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All animal studies were performed in compliance with guidelines set forth by the NIH Office of Laboratory Animal Welfare and approved by the Washington University Animal Studies Committee. In vivo biodistribution studies were performed using 185 kBq of ⁶⁴Cu-DOTA-ICP (5 μg polymer/mice) in 100 μL saline (APP Pharmaceuticals, Schaumburg, IL) injected via the tail vein of EMT6 tumor bearing mice weighing 20–25 g (n = 4/group) under inhaled isoflurane. The mice were euthanized by cervical dislocation at each time point (1, 4, 24, 48, and 72 h post-injection). Organs of interest were collected, weighed, and counted in a well gamma counter (Beckman 8000). Standards were prepared and measured along with the organs to calculate the average and standard deviation of the percentage of the injected dose per gram of tissue (% ID/g).

Su L., Dalby K.S., Luehmann H., Elkassih S.A., Cho S., He X., Detering L., Lin Y.N., Kang N., Moore D.A., Laforest R., Sun G., Liu Y, & Wooley K.L. (2022). Ultrasmall, elementary and highly translational nanoparticle X-ray contrast media from amphiphilic iodinated statistical copolymers. Acta Pharmaceutica Sinica. B, 13(4), 1660-1670.

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Biodistribution of Radiolabeled Nanoparticles in Mice

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⁶⁴Cu-CANF-comb and comb nanoparticles were reconstituted in 0.9% sodium chloride (APP pharmaceuticals) for intravenous (i.v.) injection. Wild-type C57BL/6 mice weighing 25–32 g were anesthetized with inhaled isoflurane and injected with approximately 370 kBq of the radiolabeled nanoparticles (~ 4.0 μg/kg body weight) in 100 μL saline via tail vein. The mice were re-anesthetized before euthanizing by cervical dislocation at each time point (1 h, 4 h, and 24 h, n = 4/time point) post injection (p.i.). Organs of interest were collected, weighed and counted in a well gamma counter (Beckman 8000, San Diego, CA). Standards were prepared and measured along with the samples to calculate the percentage of the injected dose per gram of tissue (% ID/gram) or percentage of the injected dose per organ of tissue (%ID/organ) (35 (link)).

Woodard P.K., Liu Y., Pressly E.D., Luehmann H.P., Detering L., Sultan D., Laforest R., McGrath A.J., Gropler R.J, & Hawker C.J. (2016). Design and Modular Construction of A Polymeric Nanoparticle for Targeted Atherosclerosis Positron Emission Tomography Imaging: A Story of 25% 64Cu-CANF-Comb. Pharmaceutical research, 33(10), 2400-2410.

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Quantitative Biodistribution of 52Mn

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At each timepoint post-administration (by injection or inhalation), animals were euthanized, dissected, and then each tissue was weighed and analyzed for radioactivity using a sodium iodide gamma-ray counter (Beckman 8000, Beckman Instruments, Irvine, California, United States). For the injection study, the injectate from the standard syringe was diluted, and one unique aliquot of this dilution was assayed after the tissues from each animal. For the inhalation study, the whole carcass of the “dose mouse” from each inhalation group was assayed once by the gamma counter. For the samples from each administration route, a single background measurement was performed, and the count rate (counts per minute, CPM) from each sample of tissue (or of diluted standard) was corrected by background subtraction and by decay correction (neglecting decay during gamma counting). The corrected CPM from the diluted standard was used to estimate the (corrected) CPM from the same mass of undiluted standard. The corrected CPM from each tissue sample was normalized both to the mass of the tissue sample (in grams, g) and to the estimated CPM from the mass of undiluted injectate that was actually delivered to the corresponding animal (injected dose, ID). Thus, the relative concentration of ⁵²Mn in each tissue sample was calculated as %ID/g.

Wooten A.L., Aweda T.A., Lewis B.C., Gross R.B, & Lapi S.E. (2017). Biodistribution and PET Imaging of pharmacokinetics of manganese in mice using Manganese-52. PLoS ONE, 12(3), e0174351.

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Biodistribution of Cu-DAPTA-Comb Nanoparticles

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Purified ⁶⁴Cu-DAPTA-Comb nanoparticles were reconstituted in 0.9% sodium chloride (APP Pharmaceuticals) for intravenous injection. Male C57BL/6 mice weighing 20–25 g (n = 4/group) were anesthetized with inhaled isoflurane, and approximately 370 kBq of labeled nanoparticle s (7.2–9.0 mg/kg of body weight) in 100 μL of saline were injected via the tail vein. The mice were reanesthetized before they were euthanized by cervical dislocation at each time point (1, 4, and 24 h) after injection. Organs of interest were collected, weighed, and counted in a well gamma counter (Beckman 8000). Standards were prepared and measured along with the samples to calculate the percentage injected dose per gram of tissue (%ID/gram).^{35 (link), 39 (link)}

Detering L., Abdilla A., Luehmann H.P., Williams J.W., Huang L.H., Sultan D., Elvington A., Heo G.S., Woodard P.K., Gropler R.J., Randolph G.J., Hawker C.J, & Liu Y. (2021). CC Chemokine Receptor 5 Targeted Nanoparticles Imaging the Progression and Regression of Atherosclerosis Using Positron Emission Tomography/Computed Tomography. Molecular pharmaceutics, 18(3), 1386-1396.

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