Male nude mice

U-87MG glioblastoma cells (2 × 10⁶) were inoculated s.c. into the hind leg flanks of athymic eight-week-old male nude mice (Envigo, Israel). Eleven days post-inoculation, tumor volume was measured (50–80 mm³) and mice were randomized into two groups (9 animals/group), treated with non-targeting siRNA (si-NT) or si- hVDAC1 (S: 238-5′-ACACUAGGCACCGAGAUUA-3′-256 and AS: 238-5′-UAAUCUCGGUGCCUAGUGU-3′) mixed with in vivo JetPEI reagent (50 nM final concentration, 2 boluses) every three days. At the end of the experiments, the mice were sacrificed, tumors were excised, and half of each tumor was either fixed and processed for IHC or frozen in liquid nitrogen for later immunoblot and RNA isolation. Experimental protocols were approved by the Institutional Animal Care and Use Committee (IL-01-08-2014).

U-87MG glioblastoma cells (3 x10⁶) were inoculated s.c. into the hind leg flanks of athymic eight-week old male nude mice (Envigo). Thirteen days post-inoculation, tumor volume was measured (100-130 mm³) and mice were randomized into three groups (5 animals/group), treated with PBS containing 0.26% DMSO or peptide in PBS, 0.26% DMSO/20 μM every second day. At the end of the experiments, the mice were sacrificed, tumors were excised and half of each tumor was either fixed and processed for IHC or frozen in liquid nitrogen for later WB.
For the intracranial-orthotopic xenograft mouse model, U-87MG cells (8×10⁴) were engrafted into a nude mouse brain using a stereotactic device. Forty eight hours after surgery, mice were randomized into three groups (6 animals/group) and treated every third day with DMSO (1.44%), Retro-Tf-D-LP4 (10mg/Kg) or Retro-Tf-D-LP4 (10mg/Kg) encapsulated by PLGA. Mice were subjected to MRI, sacrificed; brains were excised and processed for IHC. Tumor volume was analyzed using VivoQuant 2.10 software.
The experimental protocols were approved by the Institutional Animal Care and Use Committee.

DU145 cells were injected intradermally into male nude mice (Envigo), and MDA-MB-231 cells were injected intradermally into female nude mice (Envigo). Cells were injected at four sites on the ventral side of the animal, at 10⁵ cells in 10 μL of PBS. Three mice were used per treatment group. Mice either received cells that were exposed to BMS-777607 for 24 hours prior to injection, or the mice received the drug orally every day for the duration of the experiment. Three days after tumor cell injection, mice were euthanized, skin flaps were removed, and the number of blood vessels growing into the tumor nodule was quantified visually on a stereoscope.