Six males and six females (at 20 wk of age) in each group were selected to collect blood by heart punctures, and then euthanized to collect jejunal mucosal tissue at the end of the experiment. The blood was centrifuged at 4 °C for 10 min at 3000 r/min, and the serum was obtained. The contents of IgA, IgM, and IgG in serum were determined. Jejunal mucosal tissue was taken, and 9 mL normal saline was added to make tissue homogenate. The tissue was centrifuged at 4 °C for 10 min at 3500 r/min, and the supernatant was obtained. The concentrations of secreted immunoglobulin A (SIgA), interleukin-1β (IL-1β), interleukin-8 (IL-8), interleukin-10 (IL-10), interleukin-2 (IL-2), interleukin-6 (IL-6), interleukin-12 (IL-12), tumor necrosis factor-α (TNF-α), and interferon-γ (IFN-γ) were determined. All these indicators were measured using enzyme-linked immunosorbent assay (ELISA) kits (Nanjing Jiancheng Biological Engineering Research Institute, Nanjing, China), and all of them were determined by a full-wavelength micro-plate reader (Tecan Austria GmbH, Groedig, Austria).
Full wavelength microplate reader
Manufactured by Tecan
Sourced in Switzerland
The Full-wavelength microplate reader is a versatile laboratory instrument capable of measuring the absorbance of samples across a wide range of wavelengths. It provides accurate and reliable data for various applications in life science research and clinical diagnostics.
Automatically generated - may contain errors
Lab products found in correlation
Dimethyl sulfoxide (dmso), by Avantor (1 mentions)
Methyl thiazolyl tetrazolium (mtt), by Avantor (1 mentions)
Interleukin 4 (il 4), by Elabscience (1 mentions)
Ifn γ, by Elabscience (1 mentions)
Il 10, by Elabscience (1 mentions)
Gel imaging system, by Bio-Rad (1 mentions)
Vs200 digital slide scanner, by Olympus (1 mentions)
Mtt solution, by Solarbio (1 mentions)
5 protocols using full wavelength microplate reader
1
Mucosal Immune Markers in Serum and Jejunum
2
MTT Assay for Cell Viability
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HuCCT1, QBC939, and TFK1 cells were seeded on 96-well plate at a density of 5,000 cells per well (n=6). After incubation for 48 hrs, 72 hrs, and 96 hrs at 37°C, the cells were incubated with 100 uL MTT (1 mg/mL, Amresco, USA) for 4 hrs. After adding 100 µL of DMSO (Amresco, USA), the value of absorbance was measured at a wavelength of 490 nm using a full-wavelength microplate reader (TECAN, Switzerland).
3
Cytokine Levels Analysis via ELISA
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The levels of IL-2, IL-4, IL-10, and IFN-γ in the serum after allicin intervention were determined using mouse IL-2 (Elabscience, Wuhan, China), IL-4 (Elabscience), IL-10 (Elabscience), and IFN-γ (Elabscience) ELISA kits according to the manufacturer’s instructions. A full-wavelength microplate reader (Tecan, Männedorf, Switzerland) was used to measure the absorbance at a wavelength of 450 nm and calculate the concentration.
4
Protein Analysis Using Advanced Equipment
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Mini-PROTEAN Tetra electrophoresis and blots, a protein semi-dry transfer device, and a gel imaging system were used (Bio-Rad, Hercules, USA). A full wavelength microplate reader (Tecan, Zurich, Switzerland), an automatic high-speed refrigerated centrifuge (Sigma-Aldrich), a SCIENTZ-48 Tissue Grinder (Xinzhi, Hunan, China), an X-Ray Micro-CT SkyScan 1276 (Bruker, Billerica, USA), and a VS200 digital slide scanner (Olympus, Tokyo, Japan) were utilized for tissue analysis.
5
MTT Assay for PAAD Cell Viability
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PAAD cells were inoculated into 96-well plates at 100 μL per well (5×10
3 cells). MTT solution (100 μL, 5mg/mL; Solarbio, Beijing, China) was added at 0 h, 24 h, 48 h and 72 h and incubated for another 4 h. Then the supernatant was discarded and 100 μL DMSO was added to each well to dissolve the formazan crystal. After 10 min, the absorbance of each well was measured at 570 nm with a full-wavelength microplate reader (Tecan, Männedorf, Switzerland), and the cell growth curve was plotted with time as the abscissa and absorbance as the ordinate.
3 cells). MTT solution (100 μL, 5mg/mL; Solarbio, Beijing, China) was added at 0 h, 24 h, 48 h and 72 h and incubated for another 4 h. Then the supernatant was discarded and 100 μL DMSO was added to each well to dissolve the formazan crystal. After 10 min, the absorbance of each well was measured at 570 nm with a full-wavelength microplate reader (Tecan, Männedorf, Switzerland), and the cell growth curve was plotted with time as the abscissa and absorbance as the ordinate.
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